This calculator gives the amplification efficiency of a qPCR reaction based on the slope of the standard curve.

Enter the slope of your standard curve into one of the two calculators below (depending on whether Ct is on the x- or y-axis of your graph).

For a graph where log (DNA copy#) is on the x-axis and Ct on the y-axis:

For a graph where Ct is on the x-axis and log (DNA copy#) on the y-axis: