This calculator generates a pipetting table for setting up qPCR reactions with Thermo Scientific DyNAmo qPCR Kits.
- Fill in the volume of template DNA you want to use, the number of qPCR reactions you intend to run, and the individual reaction volume.
- Define if you would like to add some extra volume for pipetting. You can choose between an absolute volume, a number of extra reactions, or a percentage of the final volume.
- Fill in your primer stock concentrations and the desired final primer concentrations.
- If your reaction includes ROX passive reference dye or a probe, define the respective concentrations.
The application will automatically calculate the pipetting volumes required for a premix and the volume of the premix needed for the final reaction.
For Research Use Only. Not for use in diagnostic procedures.