DreamTaq Targeted Cube

Thermo Scientific™ DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase, available in standard and hot-start formats, that offers a balance between performance and value. DreamTaq DNA polymerases are supplied with specially optimized Thermo Scientific™ DreamTaq™ buffers that enable robust DNA amplification with minimal optimization of reaction conditions. The Thermo Scientific™ DreamTaq™ Green Buffer also supports convenient direct gel loading of PCR products. For hot-start PCR, Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is available.

Why hot-start PCR?

Better reaction outcomes:

  • Reduced nonspecific amplification
  • Increased sensitivity
  • Increased yield

Convenience with:

  • Room-temperature reaction setup
  • Reaction stability at room-temperature

Why use green?

The 10X DreamTaq Green Buffer offers the convenience of direct gel loading of PCR products. The two tracking dyes and a density reagent in the green buffer do not interfere with PCR performance, and are compatible with downstream applications including DNA sequencing, ligation, and restriction digestion.

DreamTaq Hot Start DNA Polymerase was designed for consistently robust and reliable amplification. It can help you get the results you’re looking for more easily, with virtually any template, application, or target.

DreamTaq Hot Start DNA Polymerase is the hot-start version of our enhanced DreamTaq DNA Polymerase. This hot-start DNA polymerase offers higher yields and longer amplicons than conventional Taq-based products. In addition, due to the hot-start modification, DreamTaq Hot Start DNA Polymerase has been engineered to provide increased sensitivity and specificity. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature without losing specificity or product yield.

DreamTaq Hot Start DNA Polymerase is available with either colorless or green reaction buffers, and in 2X colorless or green master mix format. Specially optimized DreamTaq buffers include Mg2+ and enable robust amplification with minimal reaction optimization. In addition, the green buffer format provides the convenience of direct loading of PCR products on a gel.

High specificity and yields from various length amplicons

DreamTaq Hot Start DNA Polymerase outperforms the competition by amplifying various fragments with high yields. Due to its strong hot-start properties, a specific fragment is amplified and no non-specific products are present (Figure 1).

Robust amplification of human genomic DNA
Figure 1. Robust amplification of human genomic DNA. DreamTaq Hot Start DNA Polymerase produces more product, cleaner bands, and longer amplicons than hot-start DNA polymerases from other suppliers. Amplification products (160 bp, 727 bp, 2 kb, or 5 kb) from human genomic DNA are shown in the figure above.
M: GeneRuler 1 kb Plus DNA Ladder;
1: DreamTaq Hot Start DNA Polymerase;
2: Promega GoTaq G2 Hot Start Polymerase;
3: NEB OneTaq Hot Start DNA Polymerase;
4: TaKaRa Taq DNA Polymerase Hot Start Version;
5: Kapa Biosystems KAPA2G Robust HotStart PCR Kit;
6: Bioline MyTaq HS DNA Polymerase.

Ideal when using limited starting material

With the ability to detect low-abundance DNA templates of sizes as small as 3 pg of human genomic DNA, DreamTaq Hot Start DNA Polymerase is ideal for use in experiments where the amount of starting material is limited or the target DNA concentration in the sample is low (Figure 2.).

High sensitivity
Figure 2. High sensitivity. DreamTaq Hot Start DNA Polymerase amplifies from lower template amounts than hot-start DNA polymerases from other suppliers. Each set of PCR reactions contained either 3 pg, 30 pg, or 3 ng of human genomic DNA.
M: GeneRuler Express DNA Ladder.
1: DreamTaq Hot Start DNA Polymerase;
2: TaKaRa Taq DNA Polymerase Hot Start Version;
3: Kapa Biosystems KAPA2G Robust HotStart PCR Kit;
4: Bioline MyTaq HS DNA Polymerase;
5: NEB OneTaq Hot Start DNA Polymerase;
6: Promega GoTaq G2 Hot Start Polymerase.

Robust yields across a wide range of amplicons

DreamTaq Hot Start DNA Polymerase is capable of amplifying a wide range of amplicons: up to 9 kb from human genomic DNA and up to 20 kb from lambda DNA (Figure 3).

Consistent and reliable amplification

Figure 3. Consistent and reliable amplification. DreamTaq Hot Start DNA Polymerase amplifies human genomic DNA with high specificity up to 9 kb amplicons. Even longer 20 kb amplicons can be amplified with lambda DNA templates.
M: Thermo Scientific™ GeneRuler™ 1 kb Plus DNA Ladder.

Technical features

  • Amplifies from as little as 3 pg human genomic DNA
  • Routinely amplifies up to 6 kb genomic DNA and 20 kb lambda DNA
  • Generates 3'-dA overhangs
  • Incorporates dUTP and modified nucleotides

Usage and applications:

  • Genotyping
  • Colony screening
  • RT-PCR
  • Generation of PCR products for TA cloning

 

The DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It helps ensure higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerases. It is supplied with a specially optimized DreamTaq Buffer or a proprietary DreamTaq Green Buffer, which enable robust DNA amplification with minimal optimization of reaction conditions.

Features

  • Robust amplification without MgCl2 optimization
  • High yields of PCR products
  • High sensitivity and amplicon length
  • Direct loading of PCR products on gels

Ready-to-load PCR products

The DreamTaq Green format is a combination of DreamTaq DNA Polymerase and the Green Reaction Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on gels. The Green Buffer does not interfere with the performance of DreamTaq DNA Polymerase and is compatible with downstream applications including DNA sequencing, ligation and restriction digestion.

Equal PCR performance with green and colorless reaction buffer   DreamTaq Green Reaction Buffer

Figure 1. Equal PCR performance with green and colorless reaction buffer.
gDNA fragments were amplified with DreamTaq and DreamTaq Green DNA Polymerase with equal efficiency. DNA Ladder: Thermo Scientific™ GeneRuler™ Express DNA Ladder.

 
Figure 2. Reaction mixtures containing
DreamTaq Green Reaction Buffer. (A) Before electrophoresis. (B) Blue and yellow tracking dyes after electrophoresis.
 

Increased PCR sensitivity and amplicon yields

DreamTaq DNA Polymerase provides higher PCR sensitivity in comparison to conventional Taq enzymes. Robust amplification can be achieved even with very low amounts of template DNA.

Superior yields with low amounts of DNA template
Figure 3. Superior yields with low amounts of DNA template. A 545 bp fragment from the human α-L-Fucosidase gene was amplified with DreamTaq DNA Polymerase and Taq DNA Polymerases from other vendors according to manufacturers’ recommendations using decreasing amounts of template DNA.
M: Thermo Scientific™ FastRuler™ Low Range DNA Ladder;
D: DreamTaq DNA Polymerase;
T: Thermo Scientific™ Taq DNA Polymerase;
1-3: Taq NA polymerases from other vendors.

Robust amplification of longer amplicons

DreamTaq DNA Polymerase outperforms conventional Taq enzymes, providing higher yields and amplifying longer amplicons. With DreamTaq DNA polymerase it is possible to amplify targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

gel image showing amplification of longer amplicons with DreamTaq DNA Polymerase

Figure 4. Amplification of longer amplicons with DreamTaq DNA Polymerase. DNA fragments of increasing length were amplified with DreamTaq DNA Polymerase and Taq DNA polymerase from other vendors according to manufacturers’ recommendations. Only DreamTaq DNA Polymerase was able to amplify the longest (5000 bp) fragment. DNA Ladder: Thermo Scientific™ GeneRuler™ Express DNA Ladder.

Videos

 How to Perform Colony PCR