PCR cloning kits, bacterial expression and ligase-independent cloning kits, enzymes and DNA vectors for efficient molecular cloning.

Molecular cloning kits

Ligase independent cloning (LIC)

LIC ensures high-cloning efficiencies of more than 95% and eliminates the need for ligation and restriction enzyme digestion steps.

DNA ligation and end repair

Kits for fast and efficient ligation as well as blunting any phosphorylation.

Recombinant protein sample preparation

Removal of N-terminal fusion tags from recombinant protein preparations.

Bacterial transformation

Quick and convenient preparation of competent E.coli cells for high transformation efficiencies.

TA and blunt-end cloning

PCR cloning based on positive selection for efficient cloning of PCR products generated with any thermostable DNA polymerase.

Direct one-step cloning of PCR products with 3'-dA overhangs generated by thermostable DNA polymerases, which lack proofreading activity.

Video: How to achieve fast and efficient ligation

How to video to demonstrate how to achieve fast and efficient ligation as part of a cloning workflow using the Thermo Scientific Rapid DNA Ligation Kit.

Related molecular cloning products

DNA ladders

Wide range of DNA Ladders for accurate DNA sizing and quantification in agarose or polyacrylamide gels.

dNTPs, NTPs & modified nucleotides

High purity nucleotides for DNA polymerization reactions.

cDNA synthesis kits

Reverse transcriptases, primers, nucleotides and and kits for efficient cDNA synthesis.

High-fidelity DNA polymerases & master mixes

Phusion High-Fidelity DNA Polymerases, kits and master mixes.

Vector & phage DNA, cloning enzymes, and reagents

Vector & phage DNA

pUC plasmid and Lambda DNA for use as molecular cloning vectors.

Molecular cloning enzymes

Enzymes for modification of DNA ends: T4 DNA ligase, alkaline phosphatase, PNK, Klenow, T4 DNA Polymerase.

Cloning reagents

IPTG and X-gal for blue/white colony selection.