Molecular cloning, a key component of the molecular biology workflow, is used to assemble recombinant DNA molecules and to direct their replication. In the molecular cloning workflow, the DNA to be cloned is identified and treated with enzymes to generate DNA fragments. These fragments are then combined with vector DNA to generate recombinant DNA molecules.
Learn more about the high-quality PCR cloning kits, bacterial expression and ligase-independent cloning kits, enzymes, and DNA vectors you need for efficient molecular cloning.
PCR cloning kits
An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Ideal for both blunt-end and sticky-end cloning.
Ligation- independent cloning (LIC)
Designed for fast and efficient LIC and tight regulation of gene expression in E. coli. LIC enables high-cloning efficiencies of more than 95% and eliminates the need for restriction enzyme digestion and ligation steps.
DNA ligation and end repair
Molecular Cloning Enzymes and Reagents
IPTG and X-gal for blue/white colony selection.
pUC plasmid and lambda DNA for use as molecular cloning vectors.
Other molecular cloning products
Complete your molecular biology workflow
Wide range of DNA Ladders for accurate DNA sizing and quantification in agarose or polyacrylamide gels.
Reverse transcriptases, primers, nucleotides and and kits for efficient cDNA synthesis.
Video: How to achieve fast and efficient ligation
How to video to demonstrate how to achieve fast and efficient ligation as part of a cloning workflow using the Thermo Scientific Rapid DNA Ligation Kit.
For Research Use Only. Not for use in diagnostic procedures.