DNA Electrophoresis—Thermo Scientific
Thermo Scientific™ DNA ladders
We offer a broad selection of DNA ladders ranging from 10 bp to 48.5 kb for accurate analysis of double-stranded DNA in agarose or polyacrylamide gels.
Loading dyes are essential for the preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels. Depending on your DNA fragment of interest you can chose between different dyes that prevent DNA masking during gel exposure to UV light.
DNA loading dyes normally contain:
- Glycerol to ensure that the sample easily sinks into the well
- EDTA, which binds divalent metal ions that may interfere with electrophoresis. By complexing metal ions, EDTA also stops metal-dependent enzymatic reactions such as DNA degradation by nucleases
- Tracking dyes (bromophenol blue, xylene cyanol FF, or orange G) to monitor the progress of electrophoresis by the migration of the dyes
|Loading dye||Description||Quantity||Catalog #|
|6X DNA Loading Dye||Two-color tracking of DNA migration during electrophoresis||5 x 1 mL||R0611|
|6X MassRuler DNA Loading Dye||One-color tracking of DNA migration during electrophoresis||5 x 1 mL||R0621|
|6X Orange DNA Loading Dye||Two-color tracking of DNA migration during electrophoresis||5 x 1 mL||R0631|
|6X DNA Loading Dye & SDS Solution||Two-color tracking of DNA migration during electrophoresis||5 x 1 mL||R1151|
Agarose is a non-toxic polysaccharide extracted from seaweed. It is easy to use and is relatively inexpensive. For electrophoresis, high-quality agarose is crucial in order to obtain sharp bands. Standard high melting point agarose is used in routine DNA electrophoresis for separation of a wide range of DNA fragments. Low melting/gelling temperature agarose is recommended for rapid DNA gel extraction with the agarose digesting enzyme Agarase. It also commonly used for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel.
Today you even have the convenience to use agarose in tablet format to omit messy weighing when preparing your gels. Try Thermo Scientific™ TopVision Agarose Tablets: high-quality, multipurpose agarose tablets in blister packaging.
Comparison of TopVision Agarose Tablets to traditional agarose powder
|Traditional agarose powder||TopVision Agarose Tablets||Advantage with TopVision Tablets|
|Gel-to-gel variation||High variability from weighing step||Tablet form delivers higher consistency hand cast gel performance||Consistent results|
|Hands-on time||Time consuming weighing step||<1 min. hands-on time— eliminate weighing step completely||Time-saving|
|Manufacturing process||Heavy dependency and use of organic solvents such as methanol, acetonitrile, DMF, heptane, etc.||Organic solvent-free process—complete elimination of any organic solvent use||Reduced environmental impact|
|Packaging||Plastic bottles||Tablet blister—bottle-free packaging, enhanced convenience and portability||Convenient|
|TopVision Agarose||100 g||R0491|
|TopVision Agarose||500 g||R0492|
|TopVision Low Melting Point Agarose||25 g||R0801|
|TopVision Agarose Tablets||200 tablets||R2801|
|TopVision Agarose Tablets||1000 tablets||R2802|
The electrophoretic mobility of DNA molecules depends on the voltage and the composition of the electrophoresis buffer. For added convenience, choose one of our concentrated solutions of Thermo Scientific™ Tris-borate and Tris-acetate electrophoresis buffers.
Which electrophoresis buffer is right for you?
|Size of the DNA||Voltage||Use this buffer|
|< 1 kb||5–10 V/cm||TBE|
|1–5 kb||4–10 V/cm||TAE or TBE|
|> 5 kb||1–3 V/cm||TAE|
|Up to 10 kb, fast electrophoresis with Express DNA ladders||Up to 23 V/cm||TAE|
|50X TAE Buffer (Tris-acetate-EDTA)||1 L||B49|
|10X TBE Buffer (Tris-borate-EDTA)||1 L||B52|
For Research Use Only. Not for use in diagnostic procedures.