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Thermo Scientific GeneRuler DNA Ladders contain mixtures of individual chromatography-purified DNA fragments, designed to deliver precision and ease of use for everyday electrophoresis.
The chromatographic method used to purify DNA fragments and used to create GeneRuler DNA ladders (Figure 1) helps enable:
Figure 1. The GeneRuler DNA ladders can offer sharp peaks during capillary electrophoresis, for exceptional purity of DNA fragments without additional DNA fragments or dNTPs.(A) Thermo Scientific GeneRuler 100 bp DNA Ladder (red) and Promega 100 bp DNA Ladder (blue). (B) GeneRuler 100 bp DNA Ladder (red) and Sigma-Aldrich DirectLoad™ PCR 100 bp Low Ladder (blue).
Figure 2. GeneRuler DNA ladders provide sharp and bright reference bands, with an easy-to-remember pattern. Amount of DNA ladder per lane was loaded according to each manufacturer’s recommendation.
(A) Lane 1: GeneRuler 1 kb DNA Ladder, ready-to-use; lane 2: GeneRuler 1 kb Plus DNA Ladder, ready-to-use; lane 3: Sigma-Aldrich DirectLoad™ Wide Range DNA Marker; lane 4: NEB Quick-Load™ 2-Log DNA Ladder (0.1–10.0 kb); lane 5: NEB Quick-Load™ 1 kb DNA Ladder.
(B) Lane 1: GeneRuler 100 bp DNA Ladder, ready-to-use; lane 2: GeneRuler 100 bp Plus DNA Ladder, ready-to-use; lane 3: Sigma-Aldrich DirectLoad™ PCR 100 bp Low Ladder; lane 4: NEB Quick-Load™ Purple 100 bp DNA Ladder.
The TriTrack DNA Loading Buffer, 6X (Cat. No. R1161) contains three dyes (bromophenol blue, xylene cyanol FF, and orange G) for visual tracking of DNA migration during electrophoresis. These dyes do not interfere with visualization.
Ready-to-use DNA ladders premixed with the TriTrack Loading Buffer are stable at room temperature up to six months.
Ambient shipping is the use of a non-insulated shipping box when products are ordered with other items that do not need freezer storage. They will be shipped in an insulated box with dry ice when orders include items that need freezer storage, to avoid shipment of multiple containers and the generation of additional waste. The GeneRuler DNA Ladder products exhibit no difference in function or stability whether shipped in ambient, gel- or dry ice conditions. Learn more about ambient shipping for Thermo Scientific DNA Ladders. For long-term stability, store DNA ladders below –20°C after you receive them.
The key to accurate band analysis is to use the correct DNA ladder for your particular application. By selecting the GeneRuler DNA Ladder of interest in Table 1, you will be directed to the corresponding product page for more details and ordering information. GeneRuler DNA Ladders are ideal for DNA sizing and approximate quantification of a wide range of double-stranded DNA ranging from 10 bp to 48.5 kb. Ready-to-use (RTU) ladders are premixed with DNA loading buffer and tracking dyes.
Table 1. Pick the best GeneRuler DNA Ladder for your analysis.*
Product name | GeneRuler Ultra Low Range DNA Ladder | GeneRuler Low Range DNA Ladder | GeneRuler 50 bp DNA Ladder | GeneRuler 100 bp DNA Ladder | GeneRuler 100 bp Plus DNA Ladder | GeneRuler 1 kb DNA Ladder | GeneRuler 1 kb Plus DNA Ladder | GeneRuler Express DNA Ladder | GeneRuler DNA Ladder Mix | GeneRuler High Range DNA Ladder |
---|---|---|---|---|---|---|---|---|---|---|
Ready-to-use format | SM1213 | SM1193 | SM0373 | SM0243 SM0244 | SM0323 SM0324 | SM0314 SM0313 | SM1333 SM1334 | SM1553 | SM0334 SM0333 | SM1353 |
Colorless DNA ladders (dye not premixed) | SM1211 SM1212 | SM1191 SM1192 | SM0371 SM0372 | SM0241 SM0242 | SM0321 SM0322 | SM0311 SM0312 | SM1331 SM1332 | SM1551 SM1552 | SM0331 SM0332 | SM1351 SM1352 |
Base pairs (bp) | 10-300 | 25-700 | 50-1000 | 100-1000 | 100-3000 | 250-10000 | 75-20000 | 100-5000 | 100-10000 | |
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* Bands represent each of the DNA fragments. The dark bands represent reference bands.
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