Images of the most commonly used GeneRuler ladders run on agarose gels.
Clean bands. Clear results.
Thermo Scientific GeneRuler DNA Ladders contain mixtures of individual chromatography-purified DNA fragments, designed to deliver precision and ease of use for everyday electrophoresis.
Thermo Scientific O’GeneRuler DNA Ladders were discontinued on June 15, 2018. Upgrade now to GeneRuler DNA Ladders featuring:
- The same sharp, clear DNA sizing standards comprised of mixtures of individual chromatography-purified DNA fragments
- Added benefit of TriTrack loading buffer with three convenient tracking dyes (Xylene Cyanol FF, Bromophenol Blue, and Orange G) to easily monitor separation of a wide range of DNA fragment sizes
- Room temperature stability for up to 6 months of ready-to-use DNA ladders
GeneRuler DNA Ladders
GeneRuler 1 kb, 100 bp, 50 bp, Mix and Express DNA Ladders
These universal ladders are for DNA sizing and approximate quantification. They cover a broad range of DNA lengths and can be used to assess the DNA amount per band. These ladders are also available in a ready-to-use format with TriTrack loading buffer premixed with the DNA solution. Options include:
- Gene Ruler 100 bp and 1kb Plus DNA Ladders—Ability to visualize a wider range of DNA fragments than 100 bp and 1 kb DNA ladders.
- GeneRuler DNA Ladder Mix—Contains high DNA band density for precise sizing of target DNA.
- GeneRuler Express DNA Ladder—Designed for fast separation (5–15 min at 23 V/cm) under a wide range of electrophoresis conditions, in different buffers, voltages, or gel percentages.
GeneRuler 1 kb, 100 bp, and 50 bp DNA ladders: selection guide
| Range (bp) | Ladder | Quantity | Cat. No. |
|---|---|---|---|
| 50–1,000 | GeneRuler 50 bp DNA Ladder | 50 µg | SM0371 |
| 5 x 50 µg | SM0372 | ||
| GeneRuler 50 bp DNA Ladder, ready-to-use | 50 µg | SM0373 | |
| 100–1,000 | GeneRuler 100 bp DNA Ladder | 50 µg | SM0241 |
| 5 x 50 µg | SM0242 | ||
| GeneRuler 100 bp DNA Ladder, ready-to-use | 50 µg | SM0243 | |
| 5 x 50 µg | SM0244 | ||
| 100–3,000 | GeneRuler 100 bp Plus DNA Ladder | 50 µg | SM0321 |
| 5 x 50 µg | SM0322 | ||
| GeneRuler 100 bp Plus DNA Ladder, ready-to-use | 50 µg | SM0323 | |
| 5 x 50 µg | SM0324 | ||
| 250–10,000 | GeneRuler 1 kb DNA Ladder | 5 x 50 µg | SM0311 |
| 25 x 50 µg | SM0312 | ||
| GeneRuler 1 kb DNA Ladder, ready-to-use | 50 µg | SM0314 | |
| 5 x 50 µg | SM0313 | ||
| 75–20,000 | GeneRuler 1 kb Plus DNA Ladder | 5 x 50 µg | SM1331 |
| 25 x 50 µg | SM1332 | ||
| GeneRuler 1 kb Plus DNA Ladder, ready-to-use | 5 x 50 µg | SM1333 | |
| 50 µg | SM1334 | ||
| 100–10,000 | GeneRuler DNA Ladder Mix | 5 x 50 µg | SM0331 |
| 25 x 50 µg | SM0332 | ||
| GeneRuler DNA Ladder Mix, ready-to-use | 50 µg | SM0334 | |
| 5 x 50 µg | SM0333 | ||
| 100–5,000 | GeneRuler Express DNA Ladder |
50 µg |
|
| 5 x 50 µg | SM1552 | ||
| GeneRuler Express DNA Ladder, ready-to-use | 50 µg | SM1553 |
GeneRuler ultra low, low, and high range ladders
- GeneRuler Ultra Low Range and Low Range DNA Ladders—Provide approximate quantification and sizing of small molecular weight DNA fragments; when analyzing siRNA, use ultra low range ladders. These ladders are available in a ready-to-use format with TriTrack loading buffer and are premixed with the DNA solution.
- GeneRuler High Range DNA Ladders— Designed for rapid sizing of high molecular weight DNA fragments (1.5 hr in 0.4% agarose gel). Available in a ready-to-use format with DNA solution premixed bromophenol blue and xylene cyanol FF as tracking dyes. The high-range ladders are not available with the TriTrack buffer.
GeneRuler ultra low range, low range, and high range ladders run on agarose and polyacrylamide gels of different concentrations.
GeneRuler low and high range DNA ladders: selection guide
| Range (bp) | Ladder | Quantity | Cat. No. |
|---|---|---|---|
| 10–300 | GeneRuler Ultra Low Range DNA Ladder | 50 µg | SM1211 |
| 5 x 50 µg | SM1212 | ||
| GeneRuler Ultra Low Range DNA Ladder, ready-to-use | 50 µg | SM1213 | |
| 25–700 | GeneRuler Low Range DNA Ladder | 50 µg | SM1191 |
| 5 x 50 µg | SM1192 | ||
| GeneRuler Low Range DNA Ladder, ready-to-use | 50 µg | SM1193 | |
| 10,171–48,502 | GeneRuler High Range DNA Ladder | 50 µg | SM1351 |
| 5 x 50 µg | SM1352 | ||
| GeneRuler High Range DNA Ladder, ready-to-use | 50 µg | SM1353 |
GeneRuler 1 kb, 100 bp, 50 bp, Mix and Express DNA Ladders
These universal ladders are for DNA sizing and approximate quantification. They cover a broad range of DNA lengths and can be used to assess the DNA amount per band. These ladders are also available in a ready-to-use format with TriTrack loading buffer premixed with the DNA solution. Options include:
- Gene Ruler 100 bp and 1kb Plus DNA Ladders—Ability to visualize a wider range of DNA fragments than 100 bp and 1 kb DNA ladders.
- GeneRuler DNA Ladder Mix—Contains high DNA band density for precise sizing of target DNA.
- GeneRuler Express DNA Ladder—Designed for fast separation (5–15 min at 23 V/cm) under a wide range of electrophoresis conditions, in different buffers, voltages, or gel percentages.
Images of the most commonly used GeneRuler ladders run on agarose gels.
GeneRuler 1 kb, 100 bp, and 50 bp DNA ladders: selection guide
| Range (bp) | Ladder | Quantity | Cat. No. |
|---|---|---|---|
| 50–1,000 | GeneRuler 50 bp DNA Ladder | 50 µg | SM0371 |
| 5 x 50 µg | SM0372 | ||
| GeneRuler 50 bp DNA Ladder, ready-to-use | 50 µg | SM0373 | |
| 100–1,000 | GeneRuler 100 bp DNA Ladder | 50 µg | SM0241 |
| 5 x 50 µg | SM0242 | ||
| GeneRuler 100 bp DNA Ladder, ready-to-use | 50 µg | SM0243 | |
| 5 x 50 µg | SM0244 | ||
| 100–3,000 | GeneRuler 100 bp Plus DNA Ladder | 50 µg | SM0321 |
| 5 x 50 µg | SM0322 | ||
| GeneRuler 100 bp Plus DNA Ladder, ready-to-use | 50 µg | SM0323 | |
| 5 x 50 µg | SM0324 | ||
| 250–10,000 | GeneRuler 1 kb DNA Ladder | 5 x 50 µg | SM0311 |
| 25 x 50 µg | SM0312 | ||
| GeneRuler 1 kb DNA Ladder, ready-to-use | 50 µg | SM0314 | |
| 5 x 50 µg | SM0313 | ||
| 75–20,000 | GeneRuler 1 kb Plus DNA Ladder | 5 x 50 µg | SM1331 |
| 25 x 50 µg | SM1332 | ||
| GeneRuler 1 kb Plus DNA Ladder, ready-to-use | 5 x 50 µg | SM1333 | |
| 50 µg | SM1334 | ||
| 100–10,000 | GeneRuler DNA Ladder Mix | 5 x 50 µg | SM0331 |
| 25 x 50 µg | SM0332 | ||
| GeneRuler DNA Ladder Mix, ready-to-use | 50 µg | SM0334 | |
| 5 x 50 µg | SM0333 | ||
| 100–5,000 | GeneRuler Express DNA Ladder |
50 µg |
|
| 5 x 50 µg | SM1552 | ||
| GeneRuler Express DNA Ladder, ready-to-use | 50 µg | SM1553 |
GeneRuler ultra low, low, and high range ladders
- GeneRuler Ultra Low Range and Low Range DNA Ladders—Provide approximate quantification and sizing of small molecular weight DNA fragments; when analyzing siRNA, use ultra low range ladders. These ladders are available in a ready-to-use format with TriTrack loading buffer and are premixed with the DNA solution.
- GeneRuler High Range DNA Ladders— Designed for rapid sizing of high molecular weight DNA fragments (1.5 hr in 0.4% agarose gel). Available in a ready-to-use format with DNA solution premixed bromophenol blue and xylene cyanol FF as tracking dyes. The high-range ladders are not available with the TriTrack buffer.
GeneRuler ultra low range, low range, and high range ladders run on agarose and polyacrylamide gels of different concentrations.
GeneRuler low and high range DNA ladders: selection guide
| Range (bp) | Ladder | Quantity | Cat. No. |
|---|---|---|---|
| 10–300 | GeneRuler Ultra Low Range DNA Ladder | 50 µg | SM1211 |
| 5 x 50 µg | SM1212 | ||
| GeneRuler Ultra Low Range DNA Ladder, ready-to-use | 50 µg | SM1213 | |
| 25–700 | GeneRuler Low Range DNA Ladder | 50 µg | SM1191 |
| 5 x 50 µg | SM1192 | ||
| GeneRuler Low Range DNA Ladder, ready-to-use | 50 µg | SM1193 | |
| 10,171–48,502 | GeneRuler High Range DNA Ladder | 50 µg | SM1351 |
| 5 x 50 µg | SM1352 | ||
| GeneRuler High Range DNA Ladder, ready-to-use | 50 µg | SM1353 |
What is the importance of chromatographic purification of DNA fragments?
Chromatographic purification of DNA ladders allows for “greener,” more environmentally friendly manufacturing processes, because no organic solvents are used in preparation of the ladders.
Figure 1 illustrates how the purification method heps enable:
- Exceptional purity and quality of DNA bands—no double peaks or broad peaks occur during capillary electrophoresis; additionally, no double bands are observed on the gel
- Accurate in-gel quantification—the concentration of each band is determined spectrophotometrically, and the exact amount of DNA in each band can be calculated precisely
Figure 1. The GeneRuler DNA ladders can offer sharp peaks during capillary electrophoresis, for exceptional purity of DNA fragments without additional DNA fragments or dNTPs. (A) Thermo Scientific GeneRuler 100 bp DNA Ladder (red) and Promega 100 bp DNA Ladder (blue). (B) GeneRuler 100 bp DNA Ladder (red) and DirectLoad PCR 100 bp Low Ladder (blue).
Ambient shipping is the use of a non-insulated shipping box when products are ordered with other items that do not need freezer storage. They will be shipped in an insulated box with dry ice when orders include items that need freezer storage, in order to avoid shipment of multiple containers and the generation of additional waste. The GeneRuler DNA Ladder products exhibit no difference in function or stability whether shipped in ambient, gel- or dry ice conditions. Learn more about ambient shipping for Thermo Scientific DNA Ladders. For long-term stability, store DNA ladders below –20°C after you receive them.
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