GeneRuler DNA Ladders

The chromatographic method used to purify DNA fragments and used to create GeneRuler DNA ladders (Figure 1) helps enable:

• Exceptional purity and quality of DNA bands—no double peaks or broad peaks occur during capillary electrophoresis; additionally, no double bands are observed on the gel.
• Accurate in-gel quantification—the concentration of each band is determined spectrophotometrically, and the exact amount of DNA in each band can be calculated precisely.
• No organic solvents are used in preparation of the ladders, helps reduce environmental waste from manufacturing process.

Ambient shipping is the use of a non-insulated shipping box when products are ordered with other items that do not need freezer storage. They will be shipped in an insulated box with dry ice when orders include items that need freezer storage, to avoid shipment of multiple containers and the generation of additional waste. The GeneRuler DNA Ladder products exhibit no difference in function or stability whether shipped in ambient, gel- or dry ice conditions. Learn more about ambient shipping for Thermo Scientific DNA Ladders. For long-term stability, store DNA ladders below –20°C after you receive them.

The key to accurate band analysis is to use the correct DNA ladder for your particular application. By selecting the GeneRuler DNA Ladder of interest in Table 1, you will be directed to the corresponding product page for more details and ordering information. GeneRuler DNA Ladders are ideal for DNA sizing and approximate quantification of a wide range of double-stranded DNA ranging from 10 bp to 48.5 kb.  Ready-to-use (RTU) ladders are premixed with DNA loading buffer and tracking dyes.

• Gene Ruler 100 bp and 1kb Plus DNA Ladders—Ability to visualize a wider range of DNA fragments than 100 bp and 1 kb DNA ladders.
• GeneRuler DNA Ladder Mix—Contains high DNA band density for precise sizing of target DNA.
• GeneRuler Express DNA Ladder—Designed for fast separation (5–15 min at 23 V/cm) under a wide range of electrophoresis conditions, in different buffers, voltages, or gel percentage.
• GeneRuler Ultra Low Range and Low Range DNA Ladders—Provide approximate quantification and sizing of small molecular weight DNA fragments; when analyzing siRNA, use ultra-low range ladders.
• GeneRuler High Range DNA Ladders—Designed for rapid sizing of high molecular weight DNA fragments (1.5 hr in 0.4% agarose gel).

Table 1. Pick the best GeneRuler DNA Ladder for your analysis.*

Product name	GeneRuler Ultra Low Range DNA Ladder	GeneRuler Low Range DNA Ladder	GeneRuler 50 bp DNA Ladder	GeneRuler 100 bp DNA Ladder	GeneRuler 100 bp Plus DNA Ladder	GeneRuler 1 kb DNA Ladder	GeneRuler 1 kb Plus DNA Ladder	GeneRuler Express DNA Ladder	GeneRuler DNA Ladder Mix	GeneRuler High Range DNA Ladder
Ready-to-use format	SM1213	SM1193	SM0373	SM0243 SM0244	SM0323 SM0324	SM0314 SM0313	SM1333 SM1334	SM1553	SM0334 SM0333	SM1353
Colorless DNA ladders (dye not premixed)	SM1211 SM1212	SM1191 SM1192	SM0371 SM0372	SM0241 SM0242	SM0321 SM0322	SM0311 SM0312	SM1331 SM1332	SM1551 SM1552	SM0331 SM0332	SM1351 SM1352
Base pairs (bp)	10-300	25-700	50-1000	100-1000	100-3000	250-10000	75-20000	100-5000	100-10000	10171-48502

* Bands represent each of the DNA fragments. The dark bands represent reference bands.