The Thermo Scientific™ conventional DNA markers are traditional molecular weight standards used for sizing and approximate quantification of linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels. The markers are composed of lambda, phage, or plasmid DNA digested to completion with the appropriate restriction enzyme(s), purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends depending on the restriction enzyme used for the marker’s preparation.


  • Sizing and approximate quantification of DNA fragments
  • Sharp bands
  • Ready-to-use versions are premixed with 6X DNA Loading Dye
  • Supplied with loading dye for sample DNA

multi-panel gel images of conventional DNA markers

Lambda, Phage & Plasmid DNA Markers selection guide

Range, bp DNA marker, marker # Quantity Catalog #
3,530–21,226 Lambda DNA/EcoRI Marker, 1 5 x 50 µg SM0281
125–23,130 Lambda DNA/HindIII Marker, 2 5 x 50 µg
25 x 50 µg SM0102
Lambda DNA/HindIII Marker, 2, ready-to-use 5 x 50 µg SM0103
125–21,226 Lambda DNA/EcoRI+HindIII Marker, 3 5 x 50 µg
25 x 50 µg SM0192
Lambda DNA/EcoRI+HindIII Marker, 3, ready-to-use 5 x 50 µg SM0193
8–587 pBR322 DNA/BsuRI (HaeIII) Marker, 5 50 µg SM0271
72–1,353 ΦX174 DNA /BsuRI (HaeIII) Marker, 9 50 µg
5 x 50 µg SM0252
ΦX174 DNA /BsuRI (HaeIII) Marker, 9, ready-to-use 50 µg SM0253
26–501 pUC19 DNA/MspI (HpaII) Marker, 23 50 µg
5 x 50 µg SM0222
pUC19 DNA/MspI (HpaII) Marker, 23, ready-to-use 50 µg SM0223