Thermo Scientific dNTPs, NTPs, and modified nucleotides have been extensively tested and verified for use in a wide variety of molecular biology applications, including PCR, real-time PCR, high-fidelity PCR, long PCR, cDNA synthesis, RT-PCR, RCA, MDA, DNA labeling, DNA sequencing, in vitro transcription, siRNA synthesis, and RNA amplification.
All dNTP and NTP formulations are designed for convenience and flexibility. Standard nucleotides are supplied as 100 mM solutions, and nucleotides mixes are available in 2mM, 10mM, 25mM or 100 mM concentration for each nucleotide in the mix.
- High purity (>99%) according to HPLC (Figure 1)
- Free from DNase, RNase, and inhibitors of qPCR, PCR and RT
- Stable for 3 years at -20oC
- Available in custom formulations and for commercial supply
Quality testing helps ensure exceptional performance of dNTPs or NTPs in your molecular biology experiments. Assessments are made using a number of analytical methods (Table 1; Figure 1).
Table 1. Qualitative parameters and specifications
|Physical||Concentration||100 + 3 mM|
|Appearance||Clear, colorless solution|
|Stability at -20oC||36 months|
|HPLC||dNTP||> 99% purity|
|NTP||> 99% purity|
|Pyrophosphate||< 0.003 pmol PPi/pmol dNTP|
|Functional||DNase, RNase and nicking activity||Not detectable|
|Bacterial DNA (qPCR, E.Coli 23S rRNA)||Not detectable|
|Human DNA contamination (qPCR, human gDNA)||Not detectable|
Figure 1. Relative HPLC profiles of ≥99% pure dNTPs. HPLC analysis shows greater than 99% triphosphate purity with undetectable mono-, di-, and tetraphosphate forms.
For Research Use Only. Not for use in diagnostic procedures.