High-fidelity PCR enzymes are used for applications requiring high accuracy during DNA amplification such as cloning, sequencing or mutagenesis. Thermo Scientific Phusion High-Fidelity DNA Polymerase was created by fusing a dsDNA-binding domain to a Pyrococcus-like proofreading polymerase. Due to this unique fusion technique, Phusion DNA Polymerases have extremely low error rates and are considered a gold standard for high-fidelity PCR.
Thermo Scientific high-fidelity DNA polymerase selection table
|Phusion High-Fidelity DNA Polymerase||Phusion Hot Start II High-Fidelity DNA Polymerase||Phusion Flash High-Fidelity PCR Master Mix||Phusion U Hot Start DNA Polymerase|
|Fidelity vs. Taq||52x||52x||25x||25x|
|Amplicon size||Up to 20 kb||Up to 20 kb||Up to 20 kb||Up to 20 kb|
*Contain green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels
Additional high-fidelity PCR enzymes:
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