High-fidelity PCR enzymes are used for applications requiring high accuracy during DNA amplification such as cloning, sequencing or mutagenesis. Thermo Scientific Phusion high-fidelity DNA polymerases were created by fusing a dsDNA-binding domain to a Pyrococcus polymerase–like proofreading enzyme. Due to this unique protein fusion engineering, Phusion DNA Polymerases have very low error rates, fast extension rates, and high inhibitor tolerance.
Phusion high-fidelity DNA polymerases are available in a variety of formats. Please reference the table below to find an appropriate Phusion format for your application.
|NEWPhusion Plus DNA Polymerase||Phusion High-Fidelity DNA Polymerase||Phusion Hot Start II DNA Polymerase||Phusion Flash High-Fidelity PCR Master Mix||Phusion U Hot Start DNA Polymerase||Phusion U Multiplex PCR Master Mix|
|Fidelity (vs. Taq enzyme)||>100x||52x||52x||25x||25x||25x|
|Tm calculation required||No||Yes||Yes||Yes||Yes||Yes|
|Enhanced specificity (hot-start version)||Yes||No||Yes||Yes||Yes||Yes|
|Amplification length||Up to 20 kb||Up to 20 kb||Up to 20 kb||Up to 20 kb||Up to 20 kb||Up to 2.5 kb|
|Speed||15–30 sec/kb||15–30 sec/kb||15–30 sec/kb||<15 sec/kb||15–30 sec/kb||15–30 sec/kb|
|GC-rich format||Yes (new GC enhanced)||Yes||Yes||No||Yes||No|
|Designed for multiplex PCR||No||No||No||No||No||Yes, up to 20 targets|
|Stand-alone enzyme format*|
|2x master mix format*|
* All stand-alone enzymes are supplied with their reaction buffers. The 2x master mix formats include all necessary PCR components except the template and primers.
** Contains green loading dyes and a density reagent for direct gel loading of PCR products
Do you need a DNA polymerase with higher fidelity and/or performance than Phusion enzymes? Discover Invitrogen Platinum SuperFi II DNA Polymerasewith >300x Taq fidelity and a simplified PCR protocol.
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