Hot-start PCR prevents the amplification of non-specific products, amplifies low abundance targets and offers convenient room temperature reaction setup. We offer different hot-start DNA polymerases to support your everyday research needs. This includes Thermo Scientific DreamTaq Hot Start DNA Polymerase, which is an enhanced hot start Taq DNA polymerase suitable for most PCR applications. DreamTaq Hot Start offers higher sensitivity, specificity, and yields compared to conventional hot start Taq DNA polymerase.
|For everyday research||For fast PCR|
|DreamTaq Hot Start |
|Phire Hot Start II |
|Sensitivity||3 pg||100 pg|
|Amplicon size–genomic/phage DNA||≤6 kb/20 kb||≤7.5 kb/20 kb|
|Cycling time||60 s/kb||10–15 s/kb|
|3′A overhang or blunt||3′A||Blunt|
*Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.
For Research Use Only. Not for use in diagnostic procedures.