Long-Range PCR Enzymes & Master Mixes—Thermo Scientific
Very long DNA fragments typically cannot be amplified using standard PCR enzymes. We offer a range of solutions enabling amplification of either extremely long (up to 47 kb) DNA fragments or very long fragments with high-fidelity and/or speed.
Thermo Scientific™ long-range PCR enzyme selection table
|Long PCR Enzyme Mix||Phusion Hot Start II High-Fidelity DNA Polymerase||Phire Hot Start II DNA Polymerase|
|Amplicon size||Up to 47 kb||Up to 20 kb||Up to 20 kb|
|Fidelity vs. Taq||3x||52x||2x|
|Extension time||45-60 s/kb||15-30 s/kb||15 s/kb|
*Contain green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels
Additional long-range PCR enzymes
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