Very long DNA fragments typically cannot be amplified using standard PCR enzymes. We offer a range of solutions enabling amplification of either extremely long (up to 47 kb) DNA fragments or very long fragments with high-fidelity and/or speed.

Thermo Scientific™ long-range PCR enzyme selection table

  Long PCR Enzyme Mix Phusion Hot Start II High-Fidelity DNA Polymerase Phire Hot Start II DNA Polymerase
Amplicon size Up to 47 kb Up to 20 kb Up to 20 kb
Fidelity vs. Taq 3x 52x 2x
Enhanced specificity
Extension time 45-60 s/kb 15-30 s/kb 15 s/kb
Stand-alone enzyme
Master mix

*Contain green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels

Additional long-range PCR enzymes