Very long DNA fragments typically cannot be amplified using standard PCR enzymes. We offer a range of solutions enabling amplification of very long fragments with high-fidelity and/or speed.

Thermo Scientific long-range PCR enzyme selection table

  Phusion Hot Start II High-Fidelity DNA Polymerase Phire Hot Start II DNA Polymerase
Amplicon size Up to 20 kb Up to 20 kb
Fidelity vs. Taq 52x 2x
Enhanced specificity
Extension time 15-30 s/kb 15 s/kb
Stand-alone enzyme
Master mix

*Contain green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels

Additional long-range PCR enzymes