Very long DNA fragments typically cannot be amplified using standard PCR enzymes. We offer a range of solutions enabling amplification of very long fragments with high-fidelity and/or speed.
Thermo Scientific long-range PCR enzyme selection table
|Phusion Hot Start II High-Fidelity DNA Polymerase||Phire Hot Start II DNA Polymerase|
|Amplicon size||Up to 20 kb||Up to 20 kb|
|Fidelity vs. Taq||52x||2x|
|Extension time||15-30 s/kb||15 s/kb|
*Contain green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels
Additional long-range PCR enzymes
PCR web tools
For Research Use Only. Not for use in diagnostic procedures.