Mesophilic and thermophilic DNA polymerases for different polymerization reactions, DNA end-blunting and amplification, labeling and others.

Properties of DNA polymerases
Polymerase Applications Template 3' - 5' exo- nuclease 5' - 3' exo- nuclease Strand displace- ment
DNA Polymerase I
  • DNA labeling by nick-translation
  • Second strand cDNA synthesis
DNA
Klenow Fragment
  • DNA blunting by fill-in 5'- overhangs
  • Random-primed DNA labeling
  • Labeling by fill-in 5'- overhangs of dsDNA
  • DNA sequencing
  • Site-specific mutagenesis
  • Second strand cDNA synthesis
DNA
Klenow Fragment, exo-
  • Random-primed DNA labeling
  • Labeling by fill-in 5'- overhangs of dsDNA
  • Strand displacement amplification
  • DNA sequencing
DNA
T4 DNA Polymerase
  • Blunting of DNA with 5'- or 3'- overhangs
  • Blunting of PCR products with 3'-dA overhangs
  • DNA labeling
  • Site-directed mutagenesis
  • Ligation-independent cloning
DNA
T7 DNA Polymerase
  • Purification of covalently closed circular DNA
  • Primer extension reactions on long templates
  • DNA 3'-end labeling
  • Strand extensions in site directed mutagenesis
  • Fill-in blunting of 5'-overhang DNA
  • Second strand cDNA synthesis
  • In situ detection of DNA fragmentation associated with apoptosis
DNA
Terminal deoxynucleotidyl transferase (TdT)
  • Production of synthetic homo-, heteropolymers
  • Homo polymeric tailing of linear duplex DNA with 3'-OH terminus
  • Oligodeoxyribonucleotide and DNA labeling
  • 5'-RACE
ssDNA
dsDNA
ssRNA
dsRNA
NA
phi29 DNA Polymerase
  • Rolling circle amplification
  • Multiple displacement amplification
  • Whole genome amplification(WGA)
  • DNA sequencing
  • Protein-primed DNA amplification
  • RNA-primed DNA amplification
DNA
Bsm DNA Polymerase
  • Loop-mediated isothermal amplification (LAMP)
  • Whole genome amplification (WGA)
  • Ramification amplification (RAM)
  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
DNA