Ligases catalyze the joining of nucleic acid molecules enzymatically in an ATP-dependent manner. T4 DNA and RNA ligases are members of a large family of ligases and are derived from the T4 bacteriophage representing the most commonly used ligases in cloning protocols.

Thermo Scientific ligase selection table

T4 DNA LigaseT4 RNA Ligase
  • Joining dsDNA with cohesive or blunt termini
  • Joining of ds oligonucleotide linkers or adaptors to dsDNA
  • Repairing nicks in duplex DNA, RNA or DNA-RNA hybrids
  • Ligase-mediated RNA detection
  • Site-directed mutagenesis
  • Amplify fragment length polymorphisms
  • RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate
  • Joining RNA to RNA
  • Circularization of synthetic oligonucleotides
  • Specific modifications of tRNAs
  • Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5'-RACE
  • Site-specific generation of composite primers for PCR
  • dsDNA
  • dsRNA
  • dsDNA-RNA hybrids
  • ds poly- and oligonucleotides
  • Oligonucleotides
  • dNTPs/NTPs
  • Phosphorylated proteins
  • ssDNA
  • ssRNA
  • poly- and oligonucleotides
Reaction catalyzedATP-dependent intra- and intermolecular formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of poly- and oligonucleotides, ssRNA and ssDNA.

ss – single-stranded
ds – double-stranded
*Although ATP is routinely used as a phosphate donor, CTP, UTP, GTP, dATP, dTTP are equally effective.

For Research Use Only. Not for use in diagnostic procedures.