Nucleases that catalyze the degradation of RNA, used to remove RNA from DNA solutions.

Properties of ribonucleases (RNases)

Ribonuclease Applications Reaction catalyzed Substrate Reaction products
RNase A, DNase and protease-free
  • Removal of RNA from plasmid or genomic DNA preparations
  • Removal of RNA from recombinant protein preparations
  • Ribonuclease protection assays
Cleavage of phosphodiester bonds between 3’-C or 3’-U residues and the 5’-OH residue of an adjacent nucleotide ssRNA
  • 3’-CMP
  • 3’-UMP
  • oligonucleotides with terminal 3’-CMP
  • 3’-UMP
RNase T1
  • Removal of RNA from DNA solutions
  • RNA sequencing
  • Ribonuclease protection assays
Cleavage of phosphodiester bonds between 3’-G residues and the 5’-OH residue of an adjacent nucleotide ssRNA
  • 3’-GMP
  • oligonucleotides with terminal 3’-GMP
RNase A/T1 Mix
  • Removal of RNA from DNA solutions 
  • Removal of RNA from recombinant protein preparations
  • Ribonuclease protection assays
Combines the activities of RNase A and RNase T1 ssRNA
  • 3’-CMP
  • 3’-UMP
  • 3’-GMP
  • oligonucleotides with terminal
    3’-CMP
  • 3’-UMP
    or 3’-GMP
RNase I
  • Removal of RNA from DNA solutions 
  • Removal of RNA from recombinant protein preparations
  • Ribonuclease protection assays
Cleavage of all phosphodiester bonds in an RNA template ssRNA
  • 3’-NMP
RNase H
  • Removal of mRNA prior to synthesis of second strand cDNA
  • Removal of RNA after first strand cDNA synthesis
  • Removal of the poly(A) sequences of mRNA after the hybridization with oligo(dt)
Cleavage of RNA phosphodiester bonds in RNA-DNA hybrids RNA in RNA-DNA hybrids
  • 5’-P oligonucleotides

ss – single-stranded
ds – double-stranded
NMP – ribonucleoside monophosphate