Nucleases that catalyze the degradation of RNA, used to remove RNA from DNA solutions.
Properties of ribonucleases (RNases)
|Ribonuclease||Applications||Reaction catalyzed||Substrate||Reaction products|
|RNase A, DNase and protease-free||Cleavage of phosphodiester bonds between 3’-C or 3’-U residues and the 5’-OH residue of an adjacent nucleotide||ssRNA|
|RNase T1||Cleavage of phosphodiester bonds between 3’-G residues and the 5’-OH residue of an adjacent nucleotide||ssRNA|
|RNase A/T1 Mix||Combines the activities of RNase A and RNase T1||ssRNA|
|RNase I||Cleavage of all phosphodiester bonds in an RNA template||ssRNA|
|RNase H||Cleavage of RNA phosphodiester bonds in RNA-DNA hybrids||RNA in RNA-DNA hybrids|
ss – single-stranded
ds – double-stranded
NMP – ribonucleoside monophosphate
For Research Use Only. Not for use in diagnostic procedures.