Conventional Restriction Enzymes—Thermo Scientific

AanI

PsiI

Optimal Buffer: Tango

TTA^TAA

B	50-100
G	50-100
O	0-20
R	0-20
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest AanI

AarI

Optimal Buffer: Unique (+oligo)

CACCTGC(4/8)^

B	NR
G	NR
O	0-20 (+oligo)
R	0-20 (+oligo)
1X Tango	NR (+oligo)
2X Tango	50-100 (+oligo)

Optimal Temperature	37°C
Double Digest	2X (+oligo)
Overnight	1
Inactivation	65° C

FastDigest:
0

AatII

Optimal Buffer: Tango

GACGT^C

B	50-100
G	20-50
O	0-20
R	0-20
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest AatII

Acc65I

Asp718I

Acc65I (Asp718I)

Optimal Buffer: O

G^GTACC

B	0-20
G	20-50
O	100
R	20-50
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest Acc65I

AdeI

DraIII

AdeI (DraIII)

Optimal Buffer: G

CACNNN^GTG

B	0-20
G	100
O	20-50
R	100
1X Tango	100*
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X* or 2X
Overnight	0.5
Inactivation	NO

FastDigest:
FastDigest AdeI

AjiI

BmgBI

AjiI (BmgBI)

Optimal Buffer: Unique

CAC^GTC

B	NR
G	NR
O	20-50*
R	NR
1X Tango	NR
2X Tango	20-50*

Optimal Temperature	37°C
Double Digest	2X*
Overnight	0.5
Inactivation	65° C

FastDigest:
0

AjuI

Optimal Buffer: R (+SAM)

^(7/12)GAA(N)7TTGG(11/6)^

B	0-20 (+SAM)
G	50-100 (+SAM)
O	20-50 (+SAM)
R	100 (+SAM)
1X Tango	50-100 (+SAM)
2X Tango	50-100 (+SAM)

Optimal Temperature	37°C
Double Digest	1X or 2X (+SAM)
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest AjuI

AloI

Optimal Buffer: R

^(7/12-13)GAAC(N)6TCC(12-13/7)^

B	0-20
G	0-20
O	0-20
R	100
1X Tango	20-50
2X Tango	100

Optimal Temperature	30°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
0

AluI

Optimal Buffer: Tango

AG^CT

B	50-100
G	0-20
O	0-20
R	0-20
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest AluI

Alw21I

BsiHKAI

Alw21I (BsiHKAI)

Optimal Buffer: O

GWGCW^C

B	0-20
G	20-50
O	100
R	50-100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Alw21I

Alw26I

BsmAI

Alw26I (BsmAI)

Optimal Buffer: Tango

GTCTC(1/5)^

B	50-100
G	100
O	0-20
R	0-20
1X Tango	100
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest Alw26I

Alw44I

ApaLI

Alw44I (ApaLI)

Optimal Buffer: Tango

G^TGCAC

B	50-100
G	100
O	0-20
R	50-100
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Alw44I

ApaI

Optimal Buffer: B

GGGCC^C

B	100
G	20-50
O	0-20
R	0-20
1X Tango	20-50
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest ApaI

BamHI

Optimal Buffer: Unique

G^GATCC

B	20-50*
G	100
O	20-50
R	50-100*
1X Tango	100*
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X* or 2X
Overnight	0.5
Inactivation	80° C (10 u)

FastDigest:
FastDigest BamHI

BauI

BssSI

BauI (BssSI)

Optimal Buffer: Tango

CACGAG(-5/-1)^

B	0-20
G	50-100
O	0-20
R	50-100
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
0

BclI

Optimal Buffer: G

T^GATCA

B	20-50
G	100
O	20-50
R	20-50
1X Tango	100*
2X Tango	100

Optimal Temperature	55°C
Double Digest	1X* or 2X
Overnight	0.1
Inactivation	80° C (10 u)

FastDigest:
FastDigest BclI

BcnI

NciI

BcnI (NciI)

Optimal Buffer: Tango

CC^SGG

B	20-50
G	50-100
O	50-100
R	50-100
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest BcnI

BcuI

SpeI

BcuI (SpeI)

Optimal Buffer: Tango

A^CTAGT

B	50-100
G	50-100
O	0-20
R	20-50
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.5
Inactivation	NO

FastDigest:
FastDigest BcuI

BfmI

SfcI

BfmI (SfcI)

Optimal Buffer: Tango

C^TRYAG

B	0-20
G	50-100
O	0-20
R	0-20
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	1
Inactivation	65° C

FastDigest:
FastDigest BfmI

BfuI

BciVI

BfuI (BciVI)

Optimal Buffer: Unique

GTATCC(6/5)^

B	NR
G	NR
O	0-20
R	0-20
1X Tango	NR
2X Tango	50-100*

Optimal Temperature	37°C
Double Digest	2X*
Overnight	1
Inactivation	80° C

FastDigest:
0

BglI

Optimal Buffer: O

GCCNNNN^NGGC

B	0-20
G	50-100
O	100
R	100
1X Tango	0-20
2X Tango	100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest BglI

BglII

Optimal Buffer: O

A^GATCT

B	0-20
G	20-50
O	100
R	50-100
1X Tango	0-20
2X Tango	100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.1
Inactivation	NO

FastDigest:
FastDigest BglII

Bme1390I

ScrFI

Bme1390I (ScrFI)

Optimal Buffer: O

CC^NGG

B	20-50
G	50-100
O	100
R	50-100
1X Tango	50-100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest Bme1390I

BoxI

PshAI

BoxI (PshAI)

Optimal Buffer: Tango

GACNN^NNGTC

B	0-20
G	0-20
O	0-20
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.5
Inactivation	80° C

FastDigest:
FastDigest BoxI

BpiI

BbsI

BpiI (BbsI)

Optimal Buffer: G

GAAGAC(2/6)^

B	20-50
G	100
O	50-100
R	50-100
1X Tango	50-100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest BpiI

BplI

Optimal Buffer: Tango(+SAM)

^(8/13)GAG(N)5CTC(13/8)^

B	0-20 (+SAM)
G	20-50 (+SAM)
O	0-20 (+SAM)
R	0-20 (+SAM)
1X Tango	100 (+SAM)
2X Tango	20-50 (+SAM)

Optimal Temperature	37°C
Double Digest	1X (+SAM)
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest BpII

Bpu10I

Optimal Buffer: Unique

CCTNAGC(-5/-2)^

B	0-20
G	20-50*
O	50-100*
R	100*
1X Tango	50-100*
2X Tango	100*

Optimal Temperature	37°C
Double Digest	1X* or 2X*
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest Bpu10l

Bpu1102I

BlpI

Bpu1102I (BlpI)

Optimal Buffer: Tango

GC^TNAGC

B	50-100
G	50-100
O	20-50
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest Bpu1102I

BseDI

BsaJI

BseDI (BsaJI)

Optimal Buffer: Tango

C^CNNGG

B	50-100
G	20-50
O	0-20
R	0-20
1X Tango	100
2X Tango	50-100

Optimal Temperature	55°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest BseDI

BseGI

BtsCI

BseGI (BtsCI)

Optimal Buffer: Tango

GGATG(2/0)^

B	20-50
G	50-100
O	20-50
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	55°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest BseGI

BseJI

BsaBI

BseJI (BsaBI)

Optimal Buffer: O

GATNN^NNATC

B	NR
G	100*
O	100
R	NR
1X Tango	100*
2X Tango	100*

Optimal Temperature	65°C
Double Digest	2X*
Overnight	0.1
Inactivation	NO

FastDigest:
FastDigest BseJI

BseLI

BslI

BseLI (BslI)

Optimal Buffer: Tango

CCNNNNN^NNGG

B	20-50
G	100
O	50-100
R	20-50
1X Tango	100
2X Tango	50-100

Optimal Temperature	55°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	NO

FastDigest:
FastDigest BseLI

BseMI

BsrDI

BseMI (BsrDI)

Optimal Buffer: R

GCAATG(2/0)^

B	0-20
G	20-50
O	0-20
R	100
1X Tango	0-20
2X Tango	50-100

Optimal Temperature	55°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	80° C

FastDigest:
FastDigest BseMI

BseMII

BspCNI

BseMII (BspCNI)

Optimal Buffer: Tango(+SAM)

CTCAG(10/8)^

B	50-100 (+SAM)
G	50-100 (+SAM)
O	50-100 (+SAM)
R	50-100 (+SAM)
1X Tango	100 (+SAM)
2X Tango	50-100 (+SAM)

Optimal Temperature	55°C
Double Digest	1X or 2X (+SAM)
Overnight	0.5
Inactivation	80° C

FastDigest:
FastDigest BseMII

BseNI

BsrI

BseNI (BsrI)

Optimal Buffer: B

ACTGG(1/-1)^

B	100
G	20-50
O	0-20
R	0-20
1X Tango	50-100
2X Tango	20-50

Optimal Temperature	65°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest BseNI

BseSI

Bme1580I

BseSI (Bme1580I)

Optimal Buffer: G

GKGCM^C

B	20-50
G	100
O	0-20
R	20-50
1X Tango	50-100
2X Tango	0-20

Optimal Temperature	55°C
Double Digest	1X
Overnight	0.1
Inactivation	80° C (10 u)

FastDigest:
FastDigest BseSI

BseXI

BbvI

BseXI (BbvI)

Optimal Buffer: Unique

GCAGC(8/12)^

B	NR
G	NR
O	NR
R	NR
1X Tango	NR
2X Tango	NR

Optimal Temperature	65°C
Double Digest	NR
Overnight	0.3
Inactivation	80° C

FastDigest:
FastDigest BseXI

Bsh1236I

BstUI

Bsh1236I (BstUI)

Optimal Buffer: R

CG^CG

B	0-20
G	0-20
O	50-100
R	100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65°C

FastDigest:
FastDigest Bsh1236I

Bsh1285I

BsiEI

Bsh1285I (BsiEI)

Optimal Buffer: G

CGRY^CG

B	20-50
G	100
O	20-50
R	0-20
1X Tango	0-20
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest Bsh1285I

BshNI

BanI

BshNI (BanI)

Optimal Buffer: O

G^GYRCC

B	0-20
G	20-50
O	100
R	50-100
1X Tango	0-20
2X Tango	100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest BshNI

BshTI

AgeI

BshTI (AgeI)

Optimal Buffer: O

A^CCGGT

B	0-20
G	20-50
O	100
R	50-100
1X Tango	20-50
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest BshTI

Bsp119I

BstBI

Bsp119I (BstBI)

Optimal Buffer: Tango

TT^CGAA

B	20-50
G	0-20
O	0-20
R	0-20
1X Tango	100
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest Bsp119I

Bsp120I

PspOMI

Bsp120I (PspOMI)

Optimal Buffer: B

G^GGCCC

B	100
G	20-50
O	0-20
R	20-50
1X Tango	50-100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest Bsp120I

Bsp1407I

BsrGI

Bsp1407I (BsrGI)

Optimal Buffer: Tango

T^GTACA

B	0-20
G	20-50
O	0-20
R	20-50
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Bsp1407I

Bsp143I

Sau3AI

Bsp143I (Sau3AI)

Optimal Buffer: Unique

^GATC

B	20-50
G	20-50
O	0-20
R	0-20
1X Tango	50-100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Bsp143I

Bsp68I

NruI

Bsp68I (NruI)

Optimal Buffer: O

TCG^CGA

B	0-20
G	20-50
O	100
R	50-100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest RruI

BspLI

NlaIV

BspLI (NlaIV)

Optimal Buffer: Tango

GGN^NCC

B	50-100
G	50-100
O	0-20
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest BspLI

BspOI

BmtI

BspOI (BmtI)

Optimal Buffer: O

GCTAG^C

B	0-20
G	0-20
O	100
R	100
1X Tango	0-20
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest BspOI

BspPI

AlwI

BspPI (AlwI)

Optimal Buffer: Tango

GGATC(4/5)^

B	20-50
G	20-50
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	55°C
Double Digest	1X
Overnight	0.5
Inactivation	80° C

FastDigest:
0

BspTI

AflII

BspTI (AflII)

Optimal Buffer: O

C^TTAAG

B	0-20
G	0-20
O	100
R	20-50
1X Tango	0-20
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest BspTI

Bst1107I

BstZ17I

Bst1107I (BstZ17I)

Optimal Buffer: O

GTA^TAC

B	20-50
G	50-100
O	100
R	100
1X Tango	20-50
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest Bst1107I

BstXI

Optimal Buffer: O

CCANNNNN^NTGG

B	20-50
G	100
O	100
R	50-100
1X Tango	50-100
2X Tango	100

Optimal Temperature	55°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest BstXI

Bsu15I

ClaI

Bsu15I (ClaI)

Optimal Buffer: Tango

AT^CGAT

B	20-50
G	20-50
O	20-50
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Bsu15I

BsuRI

HaeIII

BsuRI (HaeIII)

Optimal Buffer: R

GG^CC

B	20-50
G	20-50
O	50-100
R	100
1X Tango	50-100
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest BsuRI

BveI

BspMI

BveI (BspMI)

Optimal Buffer: O (+oligo)

ACCTGC(4/8)^

B	0-20 (+oligo)
G	20-50 (+oligo)
O	100 (+oligo)
R	20-50 (+oligo)
1X Tango	50-100 (+oligo)
2X Tango	100 (+oligo)

Optimal Temperature	37°C
Double Digest	1X or 2X (+oligo)
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest BveI

CaiI

AlwNI

CaiI (AlwNI)

Optimal Buffer: Tango

CAGNNN^CTG

B	20-50
G	20-50
O	20-50
R	50-100
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest CaiI

Cfr10I

BsrFI

Cfr10I (BsrFI)

Optimal Buffer: Unique

R^CCGGY

B	0-20
G	20-50
O	20-50
R	50-100*
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	NO

FastDigest:
FastDigest Cfr10I

Cfr13I

Sau96I

Cfr13I (Sau96I)

Optimal Buffer: Tango

G^GNCC

B	50-100
G	50-100
O	20-50
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest Cfr13I

Cfr42I

SacII

Cfr42I (SacII)

Optimal Buffer: B

CCGC^GG

B	100
G	50-100
O	0-20
R	0-20
1X Tango	50-100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.1
Inactivation	65° C

FastDigest:
0

Cfr9I

XmaI

Cfr9I (XmaI)

Optimal Buffer: Unique

C^CCGGG

B	0-20
G	0-20
O	0-20
R	0-20
1X Tango	20-50
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.2
Inactivation	65° C

FastDigest:
0

CpoI

RsrII

CpoI (RsrII)

Optimal Buffer: Tango

CG^GWCCG

B	20-50
G	50-100
O	50-100
R	20-50
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest CpoI

CseI

HgaI

CseI (HgaI)

Optimal Buffer: R

GACGC(5/10)^

B	NR
G	50-100*
O	50-100
R	100
1X Tango	100*
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X* or 2X
Overnight	0.5
Inactivation	80° C (10 u)

FastDigest:
FastDigest CseI

Csp6I

CviQI

Csp6I (CviQI)

Optimal Buffer: B

G^TAC

B	100
G	50-100
O	0-20
R	0-20
1X Tango	50-100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Csp6I

DpnI

Optimal Buffer: Tango

Gm6A^TC

B	100
G	100
O	50-100
R	50-100
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest DpnI

DraI

Optimal Buffer: Tango

TTT^AAA

B	50-100
G	50-100
O	20-50
R	20-50
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest DraI

Eam1104I

EarI

Eam1104I (EarI)

Optimal Buffer: Tango

CTCTTC(1/4)^

B	50-100
G	50-100
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest Eam1104I

Eam1105I

AhdI

Eam1105I (AhdI)

Optimal Buffer: Unique

GACNNN^NNGTC

B	20-50
G	50-100
O	0-20
R	0-20
1X Tango	50-100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Eam1105I

Ecl136II

EcoICRI

Ecl136II (EcoICRI)

Optimal Buffer: Unique

GAG^CTC

B	50-100
G	20-50
O	0-20
R	0-20
1X Tango	50-100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest Ecl136II

Eco105I

SnaBI

Eco105I (SnaBI)

Optimal Buffer: Tango

TAC^GTA

B	100*
G	50-100
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest Eco105I

Eco130I

StyI

Eco130I (StyI)

Optimal Buffer: O

C^CWWGG

B	0-20
G	20-50
O	100
R	50-100
1X Tango	50-100
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest Eco130I

Eco147I

StuI

Eco147I (StuI)

Optimal Buffer: B

AGG^CCT

B	100
G	50-100
O	20-50
R	20-50
1X Tango	50-100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest Eco147I

Eco24I

BanII

Eco24I (BanII)

Optimal Buffer: Tango

GRGCY^C

B	50-100
G	50-100
O	0-20
R	20-50
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.2
Inactivation	65° C

FastDigest:
0

Eco31I

BsaI

Eco31I (BsaI)

Optimal Buffer: G

GGTCTC(1/5)^

B	50-100
G	100
O	0-20
R	0-20
1X Tango	50-100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest Eco31I

Eco32I

EcoRV

Eco32I (EcoRV)

Optimal Buffer: R

GAT^ATC

B	0-20
G	50-100
O	50-100
R	100
1X Tango	20-50
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest Eco32I

Eco47I

AvaII

Eco47I (AvaII)

Optimal Buffer: R

G^GWCC

B	0-20
G	50-100
O	50-100
R	100
1X Tango	50-100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest Eco47I

Eco47III

AfeI

Eco47III (AfeI)

Optimal Buffer: O

AGC^GCT

B	0-20
G	20-50
O	100
R	100
1X Tango	50-100
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Eco47III

Eco52I

EagI

Eco52I (EagI)

Optimal Buffer: Unique

C^GGCCG

B	0-20
G	0-20
O	0-20
R	20-50
1X Tango	0-20
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest Eco52I

Eco57I

AcuI

Eco57I (AcuI)

Optimal Buffer: G (+SAM)

CTGAAG(16/14)^

B	100 (+SAM)
G	100 (+SAM)
O	20-50 (+SAM)
R	20-50 (+SAM)
1X Tango	50-100 (+SAM)
2X Tango	50-100 (+SAM)

Optimal Temperature	37°C
Double Digest	1X or 2X (+SAM)
Overnight	1
Inactivation	65° C

FastDigest:
FastDigest Eco57I

Eco72I

PmlI

Eco72I (PmlI)

Optimal Buffer: Tango

CAC^GTG

B	NR
G	NR
O	0-20
R	0-20
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest Eco72I

Eco81I

Bsu36I

Eco81I (Bsu36I)

Optimal Buffer: Tango

CC^TNAGG

B	50-100
G	100
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest Eco81I

Eco88I

AvaI

Eco88I (AvaI)

Optimal Buffer: Tango

C^YCGRG

B	100
G	50-100
O	0-20
R	0-20
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest Eco88I

Eco91I

BstEII

Eco91I (BstEII)

Optimal Buffer: O

G^GTNACC

B	20-50
G	20-50
O	100
R	50-100
1X Tango	NR
2X Tango	100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Eco91I

EcoO109I

DraII

EcoO109I (DraII)

Optimal Buffer: Tango

RG^GNCCY

B	50-100
G	20-50
O	20-50
R	20-50
1X Tango	100
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest EcoO109I

EcoRI

Optimal Buffer: Unique

G^AATTC

B	0-20
G	NR
O	100
R	100*
1X Tango	NR
2X Tango	100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest EcoRI

EcoRII

Optimal Buffer: O

^CCWGG

B	20-50
G	50-100
O	100
R	50-100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
0

EheI

SfoI

EheI (SfoI)

Optimal Buffer: Tango

GGC^GCC

B	20-50
G	50-100
O	0-20
R	0-20
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	1
Inactivation	65° C

FastDigest:
FastDigest EheI

Esp3I

BsmBI

Esp3I (BsmBI)

Optimal Buffer: Tango (+DTT)

CGTCTC(1/5)^

B	100 (+DTT)
G	20-50 (+DTT)
O	0-20 (+DTT)
R	0-20 (+DTT)
1X Tango	100 (+DTT)
2X Tango	0-20 (+DTT)

Optimal Temperature	37°C
Double Digest	1X (+DTT)
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest Esp3I

FaqI

BsmFI

FaqI (BsmFI)

Optimal Buffer: Tango (+SAM)

GGGAC(10/14)^

B	20-50 (+SAM)
G	20-50 (+SAM)
O	0-20 (+SAM)
R	0-20 (+SAM)
1X Tango	100 (+SAM)
2X Tango	50-100 (+SAM)

Optimal Temperature	37°C
Double Digest	1X or 2X (+SAM)
Overnight	1
Inactivation	80° C

FastDigest:
FastDigest FaqI

FspAI

Optimal Buffer: O

RTGC^GCAY

B	0-20
G	0-20
O	100
R	50-100
1X Tango	0-20
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest FspAI

FspBI

BfaI

FspBI (BfaI)

Optimal Buffer: Tango

C^TAG

B	50-100
G	20-50
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest FspBI

GsuI

BpmI

GsuI (BpmI)

Optimal Buffer: B

CTGGAG(16/14)^

B	100
G	50-100
O	20-50
R	20-50
1X Tango	100
2X Tango	50-100

Optimal Temperature	30°C
Double Digest	1X or 2X
Overnight	1
Inactivation	65° C

FastDigest:
FastDigest GsuI

HhaI

Optimal Buffer: Tango

GCG^C

B	50-100
G	50-100
O	20-50
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	NO

FastDigest:
FastDigest HhaI

Hin1I

BsaHI

Hin1I (BsaHI)

Optimal Buffer: G

GR^CGYC

B	20-50
G	100
O	20-50
R	20-50
1X Tango	20-50
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Hin1I

Hin1II

NlaIII

Hin1II (NlaIII)

Optimal Buffer: G

CATG^

B	50-100
G	100
O	20-50
R	50-100
1X Tango	50-100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest Hin1II

Hin6I

HinP1I

Hin6I (HinP1I)

Optimal Buffer: Tango

G^CGC

B	50-100
G	50-100
O	20-50
R	20-50
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Hin6I

HincII

HindII

HincII (HindII)

Optimal Buffer: Tango

GTY^RAC

B	50-100
G	50-100
O	20-50
R	50-100
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest HincII

HindIII

Optimal Buffer: R

A^AGCTT

B	0-20
G	20-50
O	0-20
R	100
1X Tango	50-100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest HindIII

HinfI

Optimal Buffer: R

G^ANTC

B	0-20
G	20-50
O	50-100
R	100
1X Tango	50-100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest HinfI

HpaII

Optimal Buffer: Tango

C^CGG

B	50-100
G	50-100
O	0-20
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest HpaII

HphI

Optimal Buffer: B

GGTGA(8/7)^

B	100
G	0-20
O	0-20
R	0-20
1X Tango	20-50
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.1
Inactivation	65° C

FastDigest:
0

Hpy8I

MjaIV

Hpy8I (MjaIV)

Optimal Buffer: Tango

GTN^NAC

B	50-100
G	50-100
O	0-20
R	20-50
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest Hpy8I

HpyF10VI

MwoI

HpyF10VI (MwoI)

Optimal Buffer: Tango

GCNNNNN^NNGC

B	0-20
G	0-20
O	0-20
R	0-20
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest HpyF10VI

HpyF3I

DdeI

HpyF3I (DdeI)

Optimal Buffer: Tango

C^TNAG

B	20-50
G	20-50
O	20-50
R	20-50
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest HpyF3I

I-SceI

Optimal Buffer: Tango

TAGGG ATAA^CAGGGTAATATCCC?TATT GTCCCATTA

B	50-100
G	50-100
O	50-100
R	50-100
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.5
Inactivation	65° C

FastDigest:
0

Kpn2I

BspEI

Kpn2I (BspEI)

Optimal Buffer: Tango

T^CCGGA

B	50-100
G	50-100
O	0-20
R	20-50
1X Tango	100
2X Tango	50-100

Optimal Temperature	55°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest Kpn2I

KpnI

Optimal Buffer: Unique

GGTAC^C

B	20-50
G	0-20
O	0-20
R	0-20
1X Tango	20-50
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest KpnI

KspAI

HpaI

KspAI (HpaI)

Optimal Buffer: B

GTT^AAC

B	100
G	50-100*
O	20-50
R	20-50
1X Tango	100*
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X* or 2X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest KspAI

LguI

SapI

LguI (SapI)

Optimal Buffer: Tango

GCTCTTC(1/4)^

B	20-50
G	50-100
O	20-50
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest LguI

Lsp1109I

BbvI

Lsp1109I (BbvI)

Optimal Buffer: Unique

GCAGC(8/12)^

B	0-20
G	20-50*
O	50-100*
R	100*
1X Tango	20-50*
2X Tango	20-50*

Optimal Temperature	37°C
Double Digest	1X* or 2X*
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest Lsp1109I

LweI

SfaNI

LweI (SfaNI)

Optimal Buffer: Tango

GCATC(5/9)^

B	0-20
G	0-20
O	0-20
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest BmsI

MauBI

Optimal Buffer: Tango

CG^CGCGCG

B	0-20
G	0-20
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest MauBI

MbiI

BsrBI

MbiI (BsrBI)

Optimal Buffer: Tango

CCGCTC(-3/-3)^

B	20-50
G	100
O	20-50
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest MbiI

MboI

Optimal Buffer: R

^GATC

B	50-100
G	50-100
O	50-100
R	100
1X Tango	50-100
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest MboI

MboII

Optimal Buffer: B

GAAGA(8/7)^

B	100
G	50-100
O	20-50
R	0-20
1X Tango	50-100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	1
Inactivation	65° C

FastDigest:
FastDigest MboII

MlsI

MscI

MlsI (MscI)

Optimal Buffer: R

TGG^CCA

B	0-20
G	20-50
O	0-20
R	100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest MlsI

MluI

Optimal Buffer: R

A^CGCGT

B	0-20
G	20-50
O	50-100
R	100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest MluI

MnlI

Optimal Buffer: G

CCTC(7/6)^

B	50-100
G	100
O	20-50
R	20-50
1X Tango	20-50
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest MnlI

Mph1103I

NsiI

Mph1103I (NsiI)

Optimal Buffer: R

ATGCA^T

B	0-20
G	50-100
O	20-50
R	100
1X Tango	50-100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest Mph1103I

MreI

Sse232I

MreI (Sse232I)

Optimal Buffer: G

CG^CCGGCG

B	20-50
G	100
O	0-20
R	0-20
1X Tango	50-100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.5
Inactivation	80° C

FastDigest:
FastDigest MreI

MspI

HpaII

MspI (HpaII)

Optimal Buffer: Tango

C^CGG

B	50-100
G	50-100
O	0-20
R	0-20
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	80° C

FastDigest:
FastDigest MspI

MssI

PmeI

MssI (PmeI)

Optimal Buffer: B

GTTT^AAAC

B	100
G	0-20
O	0-20
R	0-20
1X Tango	20-50
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest MssI

MunI

MfeI

MunI (MfeI)

Optimal Buffer: G

C^AATTG

B	100
G	100
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest MunI

Mva1269I

BsmI

Mva1269I (BsmI)

Optimal Buffer: R

GAATGC(1/-1)^

B	0-20
G	20-50
O	50-100
R	100
1X Tango	0-20
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Mva1269I

MvaI

BstNI

MvaI (BstNI)

Optimal Buffer: R

CC^WGG

B	20-50
G	20-50
O	50-100
R	100
1X Tango	20-50*
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X* or 2X
Overnight	0.1
Inactivation	NO

FastDigest:
FastDigest MvaI

Nb.Bpu10I

Optimal Buffer: R

CCTNA GCGGANT?CG

B	0-20
G	20-50
O	20-50
R	100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	80° C

FastDigest:
0

Nb.Mva1269I

Optimal Buffer: O

GAATG CCTTAC?G

B	0-20
G	20-50
O	100
R	100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	80° C

FastDigest:
0

NcoI

Optimal Buffer: Tango

C^CATGG

B	20-50
G	20-50
O	20-50
R	50-100
1X Tango	100
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest NcoI

NdeI

Optimal Buffer: O

CA^TATG

B	0-20
G	0-20
O	100
R	50-100
1X Tango	0-20
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest NdeI

NheI

Optimal Buffer: Tango

G^CTAGC

B	100
G	20-50
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest NheI

NmuCI

Tsp45I

NmuCI (Tsp45I)

Optimal Buffer: R

^GTSAC

B	0-20
G	20-50
O	50-100
R	100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest NmuCI

NotI

Optimal Buffer: O

GC^GGCCGC

B	0-20
G	0-20
O	100
R	20-50
1X Tango	0-20
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest NotI

NsbI

FspI

NsbI (FspI)

Optimal Buffer: Tango

TGC^GCA

B	20-50
G	50-100
O	0-20
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest NsbI

Nt.Bpu10I

Optimal Buffer: R

CC^TNAGCGG ANTCG

B	0-20
G	0-20
O	100
R	100
1X Tango	0-20
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.3
Inactivation	65° C

FastDigest:
0

OliI

AleI

OliI (AleI)

Optimal Buffer: R

CACNN^NNGTG

B	0-20
G	0-20
O	0-20
R	100
1X Tango	0-20
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest OliI

PacI

Optimal Buffer: Unique

TTAAT^TAA

B	20-50
G	20-50
O	0-20
R	0-20
1X Tango	0-20
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	NR
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest PacI

PaeI

SphI

PaeI (SphI)

Optimal Buffer: B

GCATG^C

B	100
G	50-100
O	0-20
R	0-20
1X Tango	50-100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest PaeI

PagI

BspHI

PagI (BspHI)

Optimal Buffer: O

T^CATGA

B	0-20
G	50-100
O	100
R	NR
1X Tango	NR
2X Tango	NR

Optimal Temperature	37°C
Double Digest	NR
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest PagI

PasI

Optimal Buffer: Unique

CC^CWGGG

B	NR
G	NR
O	NR
R	NR
1X Tango	NR
2X Tango	NR

Optimal Temperature	55°C
Double Digest	NR
Overnight	0.2
Inactivation	80° C

FastDigest:
0

PauI

BssHII

PauI (BssHII)

Optimal Buffer: R

G^CGCGC

B	0-20
G	0-20
O	100
R	100
1X Tango	0-20
2X Tango	100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest PteI

PdiI

NaeI

PdiI (NaeI)

Optimal Buffer: Tango

GCC^GGC

B	50-100
G	20-50
O	0-20
R	0-20
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.5
Inactivation	65°C

FastDigest:
FastDigest PdiI

PdmI

XmnI

PdmI (XmnI)

Optimal Buffer: Tango

GAANN^NNTTC

B	20-50
G	50-100
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest PdmI

PfeI

TfiI

PfeI (TfiI)

Optimal Buffer: O

G^AWTC

B	0-20
G	20-50
O	100
R	50-100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest PfeI

Pfl23II

BsiWI

Pfl23II (BsiWI)

Optimal Buffer: Tango

C^GTACG

B	20-50
G	50-100
O	20-50
R	20-50
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest Pfl23II

PfoI

Optimal Buffer: Tango

T^CCNGGA

B	0-20
G	20-50
O	50-100
R	0-20
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest PfoI

Ppu21I

BsaAI

Ppu21I (BsaAI)

Optimal Buffer: Unique

YAC^GTR

B	50-100*
G	100*
O	20-50
R	NR
1X Tango	NR
2X Tango	NR

Optimal Temperature	30°C
Double Digest	NR
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest Ppu21I

PscI

PciI

PscI (PciI)

Optimal Buffer: Tango

A^CATGT

B	20-50
G	20-50
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.2
Inactivation	65° C

FastDigest:
0

Psp1406I

AclI

Psp1406I (AclI)

Optimal Buffer: Tango

AA^CGTT

B	100
G	50-100
O	0-20
R	20-50
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest Psp1406I

Psp5II

PpuMI

Psp5II (PpuMI)

Optimal Buffer: G

RG^GWCCY

B	0-20
G	100
O	20-50
R	20-50
1X Tango	50-100
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest Psp5II

PstI

Optimal Buffer: O

CTGCA^G

B	50-100
G	50-100
O	100
R	100
1X Tango	50-100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	NO

FastDigest:
FastDigest PstI

PsuI

BstYI

PsuI (BstYI)

Optimal Buffer: B

R^GATCY

B	100
G	20-50
O	0-20
R	0-20
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.5
Inactivation	80° C

FastDigest:
FastDigest PsuI

PsyI

Tth111I

PsyI (Tth111I)

Optimal Buffer: B

GACN^NNGTC

B	100
G	50-100
O	0-20
R	0-20
1X Tango	50-100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest PsyI

PvuI

Optimal Buffer: R

CGAT^CG

B	0-20
G	20-50
O	50-100
R	100
1X Tango	50-100
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	80° C (10 u)

FastDigest:
FastDigest PvuI

PvuII

Optimal Buffer: G

CAG^CTG

B	50-100*
G	100
O	20-50
R	50-100
1X Tango	20-50*
2X Tango	20-50*

Optimal Temperature	37°C
Double Digest	1X* or 2X*
Overnight	0.2
Inactivation	NO

FastDigest:
FastDigest PvuII

RsaI

Optimal Buffer: Tango

GT^AC

B	50-100
G	20-50
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest RsaI

RseI

MslI

RseI (MslI)

Optimal Buffer: R

CAYNN^NNRTG

B	0-20
G	50-100
O	50-100
R	100
1X Tango	20-50
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest RseI

SacI

Optimal Buffer: Unique

GAGCT^C

B	50-100
G	20-50
O	0-20
R	0-20
1X Tango	50-100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest SacI

SalI

Optimal Buffer: O

G^TCGAC

B	0-20
G	0-20
O	100
R	20-50
1X Tango	0-20
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest SalI

SatI

Fnu4HI

SatI (Fnu4HI)

Optimal Buffer: G

GC^NGC

B	20-50
G	100
O	20-50
R	20-50
1X Tango	50-100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest SatI

ScaI

Optimal Buffer: Unique

AGT^ACT

B	0-20
G	0-20
O	0-20
R	0-20
1X Tango	0-20
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	NR
Overnight	0.5
Inactivation	80° C (10 u)

FastDigest:
FastDigest ScaI

SchI

MlyI

SchI (MlyI)

Optimal Buffer: Tango

GAGTC(5/5)^

B	20-50
G	50-100
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest SchI

SdaI

SbfI

SdaI (SbfI)

Optimal Buffer: Unique

CCTGCA^GG

B	NR
G	NR
O	0-20
R	0-20
1X Tango	NR
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.3
Inactivation	80° C

FastDigest:
FastDigest SdaI

SduI

Bsp1286I

SduI (Bsp1286I)

Optimal Buffer: Unique

GDGCH^C

B	NR
G	50-100*
O	50-100
R	0-20
1X Tango	NR
2X Tango	NR

Optimal Temperature	37°C
Double Digest	NR
Overnight	0.3
Inactivation	65° C

FastDigest:
FastDigest SduI

SfaAI

AsiSI

SfaAI (AsiSI)

Optimal Buffer: Tango

GCGAT^CGC

B	50-100
G	0-20
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest SfaAI

SfiI

Optimal Buffer: G

GGCCNNNN^NGGCC

B	50-100
G	100
O	20-50
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	50°C
Double Digest	1X
Overnight	0.2
Inactivation	NO

FastDigest:
FastDigest SfiI

SgeI

Optimal Buffer: Unique

m5CNNG(9/13)^

B	0-20
G	0-20
O	0-20
R	NR
1X Tango	NR
2X Tango	NR

Optimal Temperature	37°C
Double Digest	NR
Overnight	NR
Inactivation	65° C

FastDigest:
0

SgrDI

Optimal Buffer: R

CG^TCGACG

B	0-20
G	0-20
O	0-20
R	100
1X Tango	NR
2X Tango	100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.3
Inactivation	65° C

FastDigest:
0

SgsI

AscI

SgsI (AscI)

Optimal Buffer: Tango

GG^CGCGCC

B	0-20
G	0-20
O	0-20
R	50-100
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest SgsI

SmaI

Optimal Buffer: Tango

CCC^GGG

B	50-100
G	0-20
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	30°C
Double Digest	1X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest SmaI

SmiI

SwaI

SmiI (SwaI)

Optimal Buffer: O

ATTT^AAAT

B	0-20
G	0-20
O	100
R	20-50
1X Tango	0-20
2X Tango	20-50

Optimal Temperature	30°C
Double Digest	2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest SmiI

SmoI

SmlI

SmoI (SmlI)

Optimal Buffer: Tango

C^TYRAG

B	50-100
G	20-50
O	0-20
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	55°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	80° C

FastDigest:
0

SsiI

AciI

SsiI (AciI)

Optimal Buffer: O

CCGC(-3/-1)^

B	NR
G	20-50
O	100
R	50-100
1X Tango	NR
2X Tango	100

Optimal Temperature	37°C
Double Digest	2X
Overnight	0.5
Inactivation	65° C

FastDigest:
FastDigest AciI (SsiI)

SspDI

KasI

SspDI (KasI)

Optimal Buffer: Tango

G^GCGCC

B	20-50
G	0-20
O	NR
R	0-20
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
0

SspI

Optimal Buffer: G

AAT^ATT

B	20-50
G	100
O	0-20
R	50-100
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest SspI

TaaI

HpyCH4III

TaaI (HpyCH4III)

Optimal Buffer: Tango

ACN^GT

B	0-20
G	0-20
O	0-20
R	50-100
1X Tango	100
2X Tango	100

Optimal Temperature	65°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	NO

FastDigest:
FastDigest TaaI

TaiI

MaeII

TaiI (MaeII)

Optimal Buffer: R

ACGT^

B	50-100
G	50-100
O	20-50
R	100
1X Tango	100
2X Tango	50-100

Optimal Temperature	65°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	NO

FastDigest:
FastDigest TaiI

TaqI

Optimal Buffer: Unique

T^CGA

B	0-20
G	20-50
O	20-50
R	20-50
1X Tango	20-50
2X Tango	20-50

Optimal Temperature	65°C
Double Digest	1X or 2X
Overnight	0.3
Inactivation	NO

FastDigest:
FastDigest TaqI

TasI

Tsp509I

TasI (Tsp509I)

Optimal Buffer: B

^AATT

B	100
G	50-100
O	20-50
R	0-20
1X Tango	20-50
2X Tango	0-20

Optimal Temperature	65°C
Double Digest	1X
Overnight	0.3
Inactivation	NO

FastDigest:
FastDigest TasI

TatI

Optimal Buffer: Tango

W^GTACW

B	NR
G	50-100*
O	20-50
R	20-50
1X Tango	100*
2X Tango	0-20

Optimal Temperature	65°C
Double Digest	1X*
Overnight	0.2
Inactivation	NO

FastDigest:
FastDigest TatI

TauI

Optimal Buffer: B

GCSG^C

B	100
G	50-100
O	0-20
R	0-20
1X Tango	20-50
2X Tango	0-20

Optimal Temperature	55°C
Double Digest	1X
Overnight	1
Inactivation	NO

FastDigest:
FastDigest TauI

Tru1I

MseI

Tru1I (MseI)

Optimal Buffer: R

T^TAA

B	50-100
G	50-100
O	20-50
R	100
1X Tango	50-100
2X Tango	100

Optimal Temperature	65°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	NO

FastDigest:
FastDigest Tru1I

TscAI

TspRI

TscAI (TspRI)

Optimal Buffer: Tango

CASTG(2/-7)^

B	50-100
G	50-100
O	20-50
R	20-50
1X Tango	100
2X Tango	20-50

Optimal Temperature	65°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	NO

FastDigest:
FastDigest TscAI

Van91I

PflMI

Van91I (PflMI)

Optimal Buffer: R

CCANNNN^NTGG

B	0-20
G	50-100
O	50-100
R	100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest Van91I

VspI

AseI

VspI (AseI)

Optimal Buffer: O

AT^TAAT

B	0-20
G	50-100
O	100
R	20-50
1X Tango	100
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest VspI

XagI

EcoNI

XagI (EcoNI)

Optimal Buffer: R

CCTNN^NNNAGG

B	0-20
G	20-50
O	50-100
R	100
1X Tango	20-50
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65° C

FastDigest:
FastDigest XagI

XapI

ApoI

XapI (ApoI)

Optimal Buffer: Tango

R^AATTY

B	50-100
G	100
O	0-20
R	0-20
1X Tango	100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest XapI

XbaI

Optimal Buffer: Tango

T^CTAGA

B	50-100
G	50-100
O	20-50
R	0-20
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65°C

FastDigest:
FastDigest XbaI

XceI

NspI

XceI (NspI)

Optimal Buffer: Tango

RCATG^Y

B	50-100
G	0-20
O	0-20
R	0-20
1X Tango	100
2X Tango	0-20

Optimal Temperature	37°C
Double Digest	1X
Overnight	0.2
Inactivation	65° C

FastDigest:
FastDigest XceI

XhoI

Optimal Buffer: R

C^TCGAG

B	0-20
G	50-100
O	50-100
R	100
1X Tango	20-50
2X Tango	100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	80° C

FastDigest:
FastDigest XhoI

XmaJI

AvrII

XmaJI (AvrII)

Optimal Buffer: Tango

C^CTAGG

B	20-50
G	50-100
O	50-100
R	50-100
1X Tango	100
2X Tango	50-100

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.2
Inactivation	80° C

FastDigest:
FastDigest XmaJI

XmiI

AccI

XmiI (AccI)

Optimal Buffer: B

GT^MKAC

B	100
G	0-20
O	0-20
R	0-20
1X Tango	50-100
2X Tango	20-50

Optimal Temperature	37°C
Double Digest	1X or 2X
Overnight	0.1
Inactivation	65°C

FastDigest:
FastDigest XmiI

Conventional Restriction Enzymes—Thermo Scientific

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