Catalog #ProductIsoschizomersCut site and sequenceOverhangOptimal reaction tempe-
rature
Thermal inactivationMethylation Effects
FD0014FastDigest AluIAluBI
AG^CT
TC^GA

AGCT
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0024FastDigest Alw21IBbv12I, BsiHKAI
GWGCW^C
C^WCGWG

GWGCWC
3’ protruding ends37° C80°C, 20 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0034FastDigest Alw26IBsmAI, BstMAI
GTCTCN^NNNN
CAGAGNNNNN^

GTCTCNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0044FastDigest Alw44IApaLI, VneI
G^TGCAC
CACGT^G

GTGCAC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0054FastDigest BamHI
G^GATCC
CCTAG^G

GGATCC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0055FastDigest BamHI
G^GATCC
CCTAG^G

GGATCC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0064FastDigest BcnIAsuC2I, BpuMI, NciI
CC^SGG
GGS^CC

CCSGG
5’ protruding ends37° C80°C, 20 minDam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
FD0074FastDigest BglI
GCCNNNN^NGGC
CGGN^NNNNCCG

GCCNNNNNGGC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0083FastDigest BglII
A^GATCT
TCTAG^A

AGATCT
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired if overlapped.
FD0084FastDigest BglII
A^GATCT
TCTAG^A

AGATCT
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired if overlapped.
FD0094FastDigest Bpu1102IBlpI, Bsp1720I, CelII
GC^TNAGC
CGANT^CG

GCTNAGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0124FastDigest Bsp119IAsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV, SfuI
TT^CGAA
AAGC^TT

TTCGAA
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoBI: no effect.
FD0134FastDigest Bsp120IPspOMI
G^GGCCC
CCCGG^G

GGGCCC
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: blocked if overlapped.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0143FastDigest Bsu15IBanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI, ClaI
AT^CGAT
TAGC^TA

ATCGAT
5’ protruding ends37° C65°C, 5 minDam: blocked if overlapped.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
FD0144FastDigest Bsu15IBanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI, ClaI
AT^CGAT
TAGC^TA

ATCGAT
5’ protruding ends37° C65°C, 5 minDam: blocked if overlapped.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
FD0154FastDigest BsuRIBshFI, BsnI, BspANI, HaeIII, PhoI
GG^CC
CC^GG

GGCC
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0184FastDigest Cfr10IBse118I, BsrFI, BssAI
R^CCGGY
YGGCC^R

RCCGGY
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0194FastDigest Cfr13IAspS9I, BmgT120I, PspPI, Sau96I
G^GNCC
CCNG^G

GGNCC
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: blocked if overlapped.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0214FastDigest Csp6IAfaI, CviQI, RsaNI
G^TAC
CAT^G

GTAC
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0224FastDigest DraI
TTT^AAA
AAA^TTT

TTTAAA
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked if overlapped.
EcoBI: no effect.
FD0234FastDigest Eam1104IBst6I, EarI
CTCTTCN^NNN
GAGAAGNNNN^

CTCTTCNNNN
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0244FastDigest Eam1105IAhdI, AspEI, BmeRI, DriI, EclHKI
GACNNN^NNGTC
CTGNN^NNNCAG

GACNNNNNGTC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0254FastDigest Ecl136IIEco53kI, EcoICRI, Psp124BI, SstI
GAG^CTC
CTC^GAG

GAGCTC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
FD0264FastDigest EcoO109IDraII
RG^GNCCY
YCCNG^GR

RGGNCCY
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
FD0274FastDigest EcoRI
G^AATTC
CTTAA^G

GAATTC
5’ protruding ends37° C80°C, 5minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0275FastDigest EcoRI
G^AATTC
CTTAA^G

GAATTC
5’ protruding ends37° C80°C, 5minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0293FastDigest Eco31IBsaI, Bso31I, BspTNI
GGTCTCN^NNNN
CCAGAGNNNNN^

GGTCTCNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
FD0294FastDigest Eco31IBsaI, Bso31I, BspTNI
GGTCTCN^NNNN
CCAGAGNNNNN^

GGTCTCNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
FD0303FastDigest Eco32IEcoRV
GAT^ATC
CTA^TAG

GATATC
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0304FastDigest Eco32IEcoRV
GAT^ATC
CTA^TAG

GATATC
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0314FastDigest Eco47IAvaII, Bme18I, SinI, VpaK11BI
G^GWCC
CCWG^G

GGWCC
5’ protruding ends37° C80°C, 20 minDam: no effect.
Dcm: blocked if overlapped.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0324FastDigest Eco47IIIAfeI, Aor51HI
AGC^GCT
TCG^CGA

AGCGCT
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
FD0334FastDigest Eco52IBseX3I, BstZI, EagI, EclXI
C^GGCCG
GCCGG^C

CGGCCG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0344FastDigest Eco57IAcuI, Bsp6II, BspD6II, BspKT5I, Eco112I, Eco125I, FsfI, SstE37III
CTGAAGNNNNNNNNNNNNNNNN^
GACTTCNNNNNNNNNNNNNN^NN

CTGAAGNNNNNNNNNNNNNNNN
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0364FastDigest Eco72IAcvI, BbrPI, PmaCI, PmlI, PspCI
CAC^GTG
GTG^CAC

CACGTG
blunt37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
FD0374FastDigest Eco81IAxyI, Bse21I, Bsu36I
CC^TNAGG
GGANT^CC

CCTNAGG
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0384FastDigest Eco88IAma87I, AvaI, BmeT110I, BsiHKCI, BsoBIm
C^YCGRG
GRGCY^C

CYCGRG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
FD0394FastDigest Eco91IBstEII, BstPI, EcoO65I, PspEI
G^GTNACC
CCANTG^G

GGTNACC
5’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
FD0404FastDigest Eco105IBstSNI, SnaBI
TAC^GTA
ATG^CAT

TACGTA
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0414FastDigest Eco130IBssT1I, EcoT14I, ErhI, StyI
C^CWWGG
GGWWC^C

CCWWGG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0424FastDigest Eco147IAatI, PceI, SseBI, StuI
AGG^CCT
TCC^GGA

AGGCCT
blunt37° C80°C, 10 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
FD0434FastDigest ScaIAssI, BmcAI, ZrmI
AGT^ACT
TCA^TGA

AGTACT
blunt37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0443FastDigest EheIBbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI
GGC^GCC
CCG^CGG

GGCGCC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0444FastDigest EheIBbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI
GGC^GCC
CCG^CGG

GGCGCC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0454FastDigest Esp3IBsmBI, BstGZ53I, Esp16I, Esp23I
CGTCTCN^NNNN
GCAGAGNNNNN^

CGTCTCNNNNN
5’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
FD0464FastDigest GsuIBco35I, BpmI, Bsp22I, Bsp28I, BspJ74I, Bth1795I, Uba1437I, Uba1444I
CTGGAGNNNNNNNNNNNNNNNN^
GACCTCNNNNNNNNNNNNNN^NN

CTGGAGNNNNNNNNNNNNNNNN
3’ protruding ends30° C65°C, 5 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0474FastDigest Hin1IAcyI, BsaHI, BssNI, BstACI, Hsp92I
GR^CGYC
CYGC^RG

GRCGYC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: blocked.
EcoKI: no effect.
FD0484FastDigest Hin6IAspLEI, BstHHI, CfoI, HinP1I, HspAI
G^CGC
CGC^G

GCGC
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0494FastDigest HincIIHindII
GTY^RAC
CAR^YTG

GTYRAC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: blocked if overlapped.
EcoBI: blocked if overlapped.
FD0504FastDigest HindIII
A^AGCTT
TTCGA^A

AAGCTT
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired if overlapped.
FD0505FastDigest HindIII
A^AGCTT
TTCGA^A

AAGCTT
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0514FastDigest HpaIIBsiSI, HapII
C^CGG
GGC^C

CCGG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0524FastDigest KpnIAsp718I
GGTAC^C
C^CATGG

GGTACC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0534FastDigest Kpn2IAccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI
T^CCGGA
AGGCC^T

TCCGGA
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0544FastDigest MspIBsiSI, HapII
C^CGG
GGC^C

CCGG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0554FastDigest MvaIAjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI
CC^WGG
GGW^CC

CCWGG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0564FastDigest MluI
A^CGCGT
TGCGC^A

ACGCGT
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0573FastDigest NcoIBsp19I
C^CATGG
GGTAC^C

CCATGG
5’ protruding ends37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0574FastDigest NcoIBsp19I
C^CATGG
GGTAC^C

CCATGG
5’ protruding ends37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0575FastDigest NcoIBsp19I
C^CATGG
GGTAC^C

CCATGG
5’ protruding ends37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0583FastDigest NdeIFauNDI
CA^TATG
GTAT^AC

CATATG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0584FastDigest NdeIFauNDI
CA^TATG
GTAT^AC

CATATG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0585FastDigest NdeIFauNDI
CA^TATG
GTAT^AC

CATATG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0593FastDigest NotICciNI
GC^GGCCGC
CGCCGG^CG

GCGGCCGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0594FastDigest NotICciNI
GC^GGCCGC
CGCCGG^CG

GCGGCCGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0595FastDigest NotICciNI
GC^GGCCGC
CGCCGG^CG

GCGGCCGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0596FastDigest NotICciNI
GC^GGCCGC
CGCCGG^CG

GCGGCCGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0604FastDigest PaeIBbuI, SphI
GCATG^C
C^GTACG

GCATGC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0614FastDigest PstIBspMAI
CTGCA^G
G^ACGTC

CTGCAG
3’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0615FastDigest PstIBspMAI
CTGCA^G
G^ACGTC

CTGCAG
3’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0624FastDigest PvuIBpvUI, MvrI, Ple19I
CGAT^CG
GC^TAGC

CGATCG
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0634FastDigest PvuII
CAG^CTG
GTC^GAC

CAGCTG
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0644FastDigest SalI
G^TCGAC
CAGCT^G

GTCGAC
5’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0654FastDigest SduIBsp1286I, MhlI
GDGCH^C
C^HCGDG

GDGCHC
3’ protruding ends37° C80°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
FD0663FastDigest SmaITspMI, XmaCI, XmaI
CCC^GGG
GGG^CCC

CCCGGG
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0664FastDigest SmaITspMI, XmaCI, XmaI
CCC^GGG
GGG^CCC

CCCGGG
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0674FastDigest TaqI
T^CGA
AGC^T

TCGA
5’ protruding ends65° CNo
(column purification)
Dam: blocked if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0684FastDigest XbaI
T^CTAGA
AGATC^T

TCTAGA
5’ protruding ends37° C65°C, 20 minDam: blocked if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0685FastDigest XbaI
T^CTAGA
AGATC^T

TCTAGA
5’ protruding ends37° C65°C, 20 minDam: blocked if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0694FastDigest XhoIPaeR7I, Sfr274I, SlaI, StrI, TliI
C^TCGAG
GAGCT^C

CTCGAG
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
FD0695FastDigest XhoIPaeR7I, Sfr274I, SlaI, StrI, TliI
C^TCGAG
GAGCT^C

CTCGAG
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
FD0704FastDigest Bst1107IBssNAI, BstZ17I
GTA^TAC
CAT^ATG

GTATAC
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0714FastDigest Van91IAccB7I, BasI, PflMI
CCANNNN^NTGG
GGTN^NNNNACC

CCANNNNNTGG
3’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0724FastDigest BclIFbaI, Ksp22I
T^GATCA
ACTAG^T

TGATCA
5’ protruding ends37° CNo
(column purification)
Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0734FastDigest Mph1103IEcoT22I, NsiI, Zsp2I
ATGCA^T
T^ACGTA

ATGCAT
3’ protruding ends37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0744FastDigest CpoICspI, Rsr2I, RsrII
CG^GWCCG
GCCWG^GC

CGGWCCG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0753FastDigest MunIMfeI
C^AATTG
GTTAA^C

CAATTG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0754FastDigest MunIMfeI
C^AATTG
GTTAA^C

CAATTG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0764FastDigest Psp5IIPpuMI, PspPPI
RG^GWCCY
YCCWG^GR

RGGWCCY
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
FD0774FastDigest SspI
AAT^ATT
TTA^TAA

AATATT
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0784FastDigest Bsp143IBfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm
^GATC
CTAG^

GATC
5’ protruding ends37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0804FastDigest HinfI
G^ANTC
CTNA^G

GANTC
5’ protruding ends37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0814FastDigest MboIBfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm
^GATC
CTAG^

GATC
5’ protruding ends37° C65°C, 15 minDam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD0824FastDigest MboII
GAAGANNNNNNNN^
CTTCTNNNNNNN^N

GAAGANNNNNNNN
3’ protruding ends37° C65°C, 5 minDam: blocked if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0834FastDigest BspTIAflII, BfrI, Bst98I, BstAFI, MspCI, Vha464I
C^TTAAG
GAATT^C

CTTAAG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0854FastDigest Pfl23IIBsiWI, PspLI
C^GTACG
GCATG^C

CGTACG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0874FastDigest BseGIBstF5I, BtsCI, FokI
GGATGNN^
CCTAC^NN

GGATGNN
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0884FastDigest BseNIBse1I, BsrI, BsrSI
ACTGGN^
TGAC^CN

ACTGGN
3’ protruding ends65° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
FD0894FastDigest Bsh1285IBsiEI, BstMCI
CGRY^CG
GC^YRGC

CGRYCG
3’ protruding ends37° C80°C, 15 minDam: impaired if overlapped.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0904FastDigest Acc65IAsp718I
G^GTACC
CCATG^G

GGTACC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0914FastDigest VspIAseI, PshBI
AT^TAAT
TAAT^TA

ATTAAT
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0924FastDigest Bsh1236IAccII, BspFNI, BstFNI, BstUI, MvnI
CG^CG
GC^GC

CGCG
blunt37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD0933FastDigest Bsp1407IBsrGI, BstAUI
T^GTACA
ACATG^T

TGTACA
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0934FastDigest Bsp1407IBsrGI, BstAUI
T^GTACA
ACATG^T

TGTACA
5’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD0944FastDigest Psp1406IAclI
AA^CGTT
TTGC^AA

AACGTT
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
FD0964FastDigest Mva1269IBsaMI, BsmI, PctI
GAATGCN^
CTTAC^GN

GAATGCN
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD0973FastDigest NheIAsuNHI, BmtI
G^CTAGC
CGATC^G

GCTAGC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0974FastDigest NheIAsuNHI, BmtI
G^CTAGC
CGATC^G

GCTAGC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD0984FastDigest Tru1IMseI, Tru9I
T^TAA
AAT^T

TTAA
5’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked if overlapped.
EcoBI: no effect.
FD0994FastDigest AatIIZraI
GACGT^C
C^TGCAG

GACGTC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1004FastDigest BshNIAccB1I, BanI, BspT107I
G^GYRCC
CCRYG^G

GGYRCC
5’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoBI: no effect.
FD1014FastDigest BpiIBbsI, BpuAI, BstV2I
GAAGACNN^NNNN
CTTCTGNNNNNN^

GAAGACNNNNNN
5’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1024FastDigest BstXI
CCANNNNN^NTGG
GGTN^NNNNNACC

CCANNNNNNTGG
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1034FastDigest KspAIHpaI
GTT^AAC
CAA^TTG

GTTAAC
blunt37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: blocked if overlapped.
EcoBI: no effect.
FD1074FastDigest MnlI
CCTCNNNNNNN^
GGAGNNNNNN^N

CCTCNNNNNNN
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD1084FastDigest BseDIBsaJI, BssECI
C^CNNGG
GGNNC^C

CCNNGG
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1124FastDigest RsaIAfaI, CviQI, RsaNI
GT^AC
CA^TG

GTAC
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1133FastDigest SacIEco53kI, EcoICRI, Psp124BI, SstI
GAGCT^C
C^TCGAG

GAGCTC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1134FastDigest SacIEco53kI, EcoICRI, Psp124BI, SstI
GAGCT^C
C^TCGAG

GAGCTC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1144FastDigest TaiIHpyCH4IV, MaeII
ACGT^
^TGCA

ACGT
3’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: blocked.
FD1154FastDigest BspLIBmiI, NlaIV, PspN4I
GGN^NCC
CCN^NGG

GGNNCC
blunt37° C65°C, 20 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1164FastDigest BfmIBstSFI, SfcI
C^TRYAG
GAYRT^C

CTRYAG
5’ protruding ends37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1184FastDigest Bpu10I
CC^TNAGC
GGANT^CG

CCTNAGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1194FastDigest SdaISbfI, Sse8387I
CCTGCA^GG
GG^ACGTCC

CCTGCAGG
3’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1204FastDigest BseLIAfiI, Bsc4I, BslI
CCNNNNN^NNGG
GGNN^NNNNNCC

CCNNNNNNNGG
3’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1214FastDigest MlsIBalI, MluNI, MscI, Msp20I
TGG^CCA
ACC^GGT

TGGCCA
blunt37° C80°C, 20 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1224FastDigest NsbIAcc16I, AviII, FspI
TGC^GCA
ACG^CGT

TGCGCA
blunt37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1234FastDigest AdeIDraIII
CACNNN^GTG
GTG^NNNCAC

CACNNNGTG
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
FD1244FastDigest SmiISwaI
ATTT^AAAT
TAAA^TTTA

ATTTAAAT
blunt37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1253FastDigest BcuIAhlI, SpeI
A^CTAGT
TGATC^A

ACTAGT
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
FD1254FastDigest BcuIAhlI, SpeI
A^CTAGT
TGATC^A

ACTAGT
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
FD1264FastDigest BseMIBse3DI, BsrDI
GCAATGNN^
CGTTAC^NN

GCAATGNN
3’ protruding ends55° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1274FastDigest MbiIAccBSI, BsrBI
CCG^CTC
GGC^GAG

CCGCTC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
FD1284FastDigest PagICciI, BspHI, RcaI
T^CATGA
AGTAC^T

TCATGA
5’ protruding ends37° C80°C, 5 minDam: impaired if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired if overlapped.
FD1294FastDigest TatI
W^GTACW
WCATG^W

WGTACW
5’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1304FastDigest XagIBstENI, EcoNI
CCTNN^NNNAGG
GGANNN^NNTCC

CCTNNNNNAGG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1314FastDigest BplI
GAAGACNN^NNNN
CTTCTGNNNNNN^

GAAGACNNNNNN
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1334FastDigest PsyIAspI, PflFI, Tth111I
GACN^NNGTC
CTGNN^NCAG

GACNNNGTC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1344FastDigest MssIPmeI
GTTT^AAAC
CAAA^TTTG

GTTT^AAAC
blunt37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked if overlapped.
EcoBI: no effect.
FD1354FastDigest TasISse9I, Tsp509I, TspEI
^AATT
TTAA^>

AATT
5’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked if overlapped.
FD1364FastDigest TaaIBst4CI, HpyCH4III
ACN^GT
TG^NCA

ACNGT
3’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
FD1374FastDigest SchIMlyI, PleI, PpsI, SchI
GAGTCNNNNN^
CTCAGNNNNN

GAGTCNNNNN
blunt37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1383FastDigest XapIAcsI, ApoI
R^AATTY
YTTAA^R

RAATTY
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1384FastDigest XapIAcsI, ApoI
R^AATTY
YTTAA^R

RAATTY
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1394FastDigest CaiIAlwNI
CAGNNN^CTG
GTC^NNNGAC

CAGNNNCTG
3’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: blocked if overlapped.
CpG: no effect.
EcoKI: no effect.
FD1404FastDigest BseMIIBspCNI
GCAATGNN^
CGTTAC^NN

GCAATGNN
3’ protruding ends55° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1414FastDigest ApaIPspOMI
GGGCC^C
C^CCGGG

GGGCCC
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1424FastDigest Bme1390IBmrFI, BssKI, BstSCI, MspR9I, ScrFI, StyD4I
CC^NGG
GGN^CC

CCNGG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: blocked if overlapped.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1434FastDigest BoxIBstPAI, PshAI
GACNN^NNGTC
CTGNN^NNCAG

GACNNNNGTC
blunt37° C80°C, 20 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
FD1444FastDigest BseSIBaeGI, BstSLI
GKGCM^C
C^MCGKG

GKGCMC
3’ protruding ends37° C80°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: impaired if overlapped.
EcoBI: no effect.
FD1454FastDigest BseXIBbvI, BstV1I
GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^

GCAGCNNNNNNNNNNNN
5’ protruding ends65° C80°C, 15 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1464FastDigest BshTIAgeI, AsiGI, CspAI, PinAI
A^CCGGT
TGGCC^A

ACCGGT
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
FD1474FastDigest XceIBstNSI, NspI
RCATG^Y
Y^GTACR

RCATGY
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1484FastDigest XmiIAccI, FblI
GT^MKAC
CAKM^TG

GTMKAC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1514FastDigest NmuCITsp45I
^GTSAC
CASTG^

GTSAC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
FD1524FastDigest PdiIMroNI, NaeI, NgoMIV
GCC^GGC
CGG^CCG

GCCGGC
blunt37° C65°C, 15 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1534FastDigest PdmIAsp700I, MroXI, XmnI
GAANN^NNTTC
CTTNN^NNAAG

GAANNNNTTC
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
FD1554FastDigest PsuIBstX2I, BstYI, MflI, XhoII
R^GATCY
YCTAG^R

RGATCY
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1564FastDigest XmaJIAspA2I, AvrII, BlnI
C^CTAGG
GGATC^C

CCTAGG
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1574FastDigest Hpy8IHpy166II
GTN^NAC
CAN^NTG

GTNNAC
blunt37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
FD1634FastDigest OliIAleI
CACNN^NNGTG
GTGNN^NNCAC

CACNNNNGTG
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: blocked if overlapped.
EcoBI: blocked if overlapped.
FD1644FastDigest SatIFnu4HI, Fsp4HI, ItaI
GC^NGC
CGN^CG

GCNGC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1654FastDigest TauI
GCSG^C
C^GSCG

GCSGC
3’ protruding ends55° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1664FastDigest FspAI
RTGC^GCAY
YACG^CGTR

RTGCGCAY
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
FD1703FastDigest DpnIMalI
GA^TC
CT^AG

GATC
blunt37° C80°C, 5 minDam: does not cut dam- DNA.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1704FastDigest DpnIMalI
GA^TC
CT^AG

GATC
blunt37° C80°C, 5 minDam: does not cut dam - DNA.
Dcm: no effect.
Cpg: no effect.
EcoKI: no effect.
FD1714FastDigest BseJIBsaBI, Bse8I
GATNN^NNATC
CTANN^NNTAG

GATNNNNATC
blunt37° CNo
(column purification)
Dam: blocked if overlapped.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1724FastDigest AasIDrdI, DseDI
GACNNNN^NNGTC
CTGNN^NNNNCAG

GACNNNNNNGTC
3’ protruding ends37° C80°C, 10 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1734FastDigest HpyF10VIBstMWI, MwoI
GCNNNNN^NNGC
CGNN^NNNNNCG

GCNNNNNNNGC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1744FastDigest BveIAcc36I, BfuAI, BspMI
ACCTGCNNNN^NNNN
TGGACGNNNNNNNN^

ACCTGCNNNNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
FD1754FastDigest PfoI
T^CCNGGA
AGGNCC^T

TCCNGGA
5’ protruding ends37° C65°C, 5 minDam: impaired if overlapped.
Dcm: blocked if overlapped.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1764FastDigest FspBIBfaI, MaeI, XspI
C^TAG
GAT^C

CTAG
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1784FastDigest PfeITfiI
G^AWTC
CTWA^G

GAWTC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
FD1794FastDigest SsiIAciI, BspACI
C^CGC
GGC^G

CCGC
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1814FastDigest FaqIBslFI, BsmFI
GGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^

GGGACNNNNNNNNNNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1824FastDigest SfiI
GGCCNNNN^NGGCC
CCGGN^NNNNCCGG

GGCCNNNNNGGCC
3’ protruding ends50° CNo
(column purification)
Dam: no effect.
Dcm: impaired if overlapped.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD1834FastDigest Hin1IICviAII, FaeI, FatI, Hsp92II, NlaIII
CATG^
^GTAC

CATG
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1854FastDigest HhaIAspLEI, BstHHI, CfoI, HinP1I, HspAI
GCG^C
C^GCG

GCGC
3’ protruding ends37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1884FastDigest HpyF3IBstDEI, DdeI
C^TNAG
GANT^C

CTNAG
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1894FastDigest SgsIAscI, PalAI
GG^CGCGCC
CCGCGC^GG

GGCGCGCC
5’ protruding ends37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD1904FastDigest CseIHgaI
GACGCNNNNN^NNNNN
CTGCGNNNNNNNNNN^

GACGCNNNNNNNNNN
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
FD1934FastDigest LguIBspQI, PciSI, SapI
GCTCTTCN^NNN
CGAGAAGNNNN^

CTCTTCNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD1954FastDigest AjuI
NNNNN^NNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN^
^NNNNNNNNNNNNCTTNNNNNNNAACCNNNNNN^NNNNN

NNNNNNNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD1974FastDigest Ppu21IBsaAI, BstBAI
YAC^GTR
RTG^CAY

YACGTR
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked if overlapped.
FD2004FastDigest RseIMslI, SmiMI
CAYNN^NNRTG
GTRNN^NNYAC

CAYNNNNRTG
blunt37° C65°C, 20 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked if overlapped.
FD2024FastDigest MreI
CG^CCGGCG
GCGGCC^GC

CGCCGGCG
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD2044FastDigest BspOIAsuNHI, BmtI
GCTAG^C
C^GATCG

GCTAGC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
FD2064FastDigest AanIPsiI
TTA^TAA
AAT^ATT

TTATAA
blunt37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoBI: no effect.
FD2074FastDigest Lsp1109IBbvI, BstV1I
GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^

GCAGCNNNNNNNNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD2084FastDigest MauBI
CG^CGCGCG
GCGCGC^GC

CGCGCGCG
3’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD2094FastDigest SfaAIAsiSI, RgaI, SgfI
GCGAT^CGC
CGC^TAGCG

GCGATCGC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD2104FastDigest TscAITspRI
NNCASTGNN^
^NNGTSACNN

NNCASTGNN
3’ protruding ends65° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: no effect.
FD2114FastDigest CsiIMabI, SexAI
A^CCWGGT
TGGWCC^A

ACCWGGT
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
FD2124FastDigest BmsIBhaI, BscAI, BscUI, BsmNI, BspST5I, Bst19I, CjeP338II, LweI, Mva1312II, PhaI, SfaNI
GCATCNNNNN^NNNN
CGTAGNNNNNNNNN^

GCATCNNNNNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
FD2134FastDigest PteIBsePIm BssHII
G^CGCGC
CGCGC^G

GCGCGC
5’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD2144FastDigest FokIBstF5I, BtsCI, FokI
GGATGNNNNNNNNN^NNNN
CCTACNNNNNNNNNNNNN^

GGATGNNNNNNNNNNNNN
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
FD2154FastDigest RruIBtuMI, NruI
TCG^CGA
AGC^GCT

TCGCGA
blunt37° CNo
(column purification)
Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
FD2164FastDigest KflISse1825I, SanDI
GG^GWCCC
CCCWG^GG

GGGWCCC
5’ protruding ends37° CNo
(column purification)
Dam: no effect.
CpG: blocked if overlapped.
EcoKI: no effect.
EcoBI: no effect.
FD2174FastDigest SaqAIMseI, Tru1I, Tru9I
T^TAA
AAT^T

TTAA
5’ protruding ends37° C65°C, 5 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoBI: no effect.
FD2184FastDigest BfoIAccB2I, Bme142I, BsmHI, Bsp143II, Bst16I, Bst1473II, BstH2I, Btu34II, HaeII, HinHI, LpnI, NgoAI, NgoBI, NgoCI, NgoGI, NgoJI, NgoMI, NgoWI
RGCGC^Y
Y^CGCGR

RGCGCY
3’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
FD2204FastDigest PacI
TTAAT^TAA
AAT^TAATT

TTAATTAA
3’ protruding ends37° C65°C, 10 minDam: no effect.
Dcm: no effect.
CpG: no effect.
EcoBI: no effect.
FD2224FastDigest PspFI
CCCAG^C
G^GGTCG

CCCAGC
3’ protruding ends37° C80°C, 5 minDam: no effect.
Dcm: no effect.
CpG: impaired if overlapped.
EcoKI: no effect.
EcoBI: no effect.