Reverse transcription (RT) is a widely used application in molecular biology research for RNA and expression analysis. The Thermo Scientific reverse transcription portfolio offers robust and reliable reverse transcriptases in a variety of optimized formats for efficiency in RT-PCR and RT-qPCR applications.
Thermo Scientific reverse transcription products
Selecting the right RT for cDNA synthesis is critical to detecting low-abundance RNAs or dealing with RNA that contains high amounts of secondary structure. Considerations for selecting the RT should include sensitivity and thermostability (ability to perform at higher temperature).
Reverse transcriptases
Stand-alone enzymes engineered for optimal cDNA synthesis
Enhanced performance | Routine performance | |||
---|---|---|---|---|
Product | Maxima H Minus Reverse Transcriptase | Maxima Reverse Transcriptase | RevertAid H Minus Reverse Transcriptase | RevertAid Reverse Transcriptase |
Active temperature range | 50–65 oC | 50–65 oC | 42–55 oC | 42–50 oC |
RNase H activity | No | Yes | No | Yes |
Reaction time | 15-30 min | 15-30 min | 60 min | 60 min |
Sensitivity (total RNA) | 1 pg | 1 pg | 0.1 ng | 0.1 ng |
Maximum cDNA length | 20 kb | 20 kb | 13 kb | 13 kb |
First strand cDNA synthesis kits/master mixes
Complete kits for efficient and reproducible first strand cDNA synthesis
Enhanced performance | Routine performance | |||
---|---|---|---|---|
Product | Maxima H Minus First Strand cDNA Synthesis Master Mixes | Maxima First Strand cDNA Synthesis Kit | RevertAid H Minus First Strand cDNA Synthesis Kit | RevertAid First Strand cDNA Synthesis Kit |
Active temperature range | 50–65 oC | 50–65 oC | 42–55 oC | 42–50 oC |
RNase H activity | No | Yes | No | Yes |
Reaction time | 15-30 min | 15-30 min | 60 min | 60 min |
Formats with dsDNase* | Yes | Yes | N/A | N/A |
Sensitivity (total RNA) | 1 pg | 1 pg | 0.1 ng | 0.1 ng |
Maximum cDNA length | 20 kb | 20 kb | 13 kb | 13 kb |
* dsDNase step is a quick (2 min) and easy gDNA removal step prior to RT step. |
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Tailor-made performance tested products with strict quality standards to meet your molecular assay requirements
For Research Use Only. Not for use in diagnostic procedures.