Reverse transcription

Reverse transcription (RT) is a widely used application in molecular biology research for RNA and expression analysis. The Thermo Scientific reverse transcription portfolio offers robust and reliable reverse transcriptases in a variety of optimized formats for efficiency in RT-PCR and RT-qPCR applications.

Thermo Scientific reverse transcription products

Selecting the right RT for cDNA synthesis is critical to detecting low-abundance RNAs or dealing with RNA that contains high amounts of secondary structure. Considerations for selecting the RT should include sensitivity and thermostability (ability to perform at higher temperature).

Reverse transcriptases

Stand-alone enzymes engineered for optimal cDNA synthesis

 Enhanced performanceRoutine performance
ProductMaxima H Minus Reverse TranscriptaseMaxima Reverse TranscriptaseRevertAid H Minus Reverse TranscriptaseRevertAid Reverse Transcriptase
Active temperature range50–65 oC50–65 oC42–55 o42–50 oC
RNase H activityNoYesNoYes
Reaction time15-30 min15-30 min60 min60 min
Sensitivity (total RNA)1 pg1 pg0.1 ng0.1 ng
Maximum cDNA length20 kb20 kb13 kb13 kb

First strand cDNA synthesis kits/master mixes

Complete kits for efficient and reproducible first strand cDNA synthesis

 Enhanced performanceRoutine performance
ProductMaxima H Minus First Strand cDNA Synthesis Master MixesMaxima First Strand cDNA Synthesis KitRevertAid H Minus First Strand cDNA Synthesis KitRevertAid First Strand cDNA Synthesis Kit
Active temperature range50–65 oC50–65 oC42–55 o42–50 oC
RNase H activityNoYesNoYes
Reaction time15-30 min15-30 min60 min60 min
Formats with dsDNase*YesYesN/AN/A
Sensitivity (total RNA)1 pg1 pg0.1 ng0.1 ng
Maximum cDNA length20 kb20 kb13 kb13 kb
* dsDNase step is a quick (2 min) and easy gDNA removal step prior to RT step.

Ready-to-use primers

Support

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Email or call our technical application scientists for additional questions regarding reverse transcription enzymes and master mixes.

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OEM and custom solutions

Tailor-made performance tested products with strict quality standards to meet your molecular assay requirements