Reverse transcription (RT) is a widely used application in molecular biology research for RNA and expression analysis. The Thermo Scientific reverse transcription portfolio offers robust and reliable reverse transcriptases in a variety of optimized formats for efficiency in RT-PCR and RT-qPCR applications.
Thermo Scientific reverse transcription products
Selecting the right RT for cDNA synthesis is critical to detecting low-abundance RNAs or dealing with RNA that contains high amounts of secondary structure. Considerations for selecting the RT should include sensitivity and thermostability (ability to perform at higher temperature).
Stand-alone enzymes engineered for optimal cDNA synthesis
| Enhanced performance | Routine performance | |||
|---|---|---|---|---|
| Product | Maxima H Minus Reverse Transcriptase | Maxima Reverse Transcriptase | RevertAid H Minus Reverse Transcriptase | RevertAid Reverse Transcriptase |
| Active temperature range | 50–65 oC | 50–65 oC | 42–55 oC | 42–50 oC |
RNase H activity |
No | Yes | No | Yes |
| Reaction time | 15-30 min | 15-30 min | 60 min | 60 min |
Sensitivity (total RNA) |
1 pg | 1 pg | 0.1 ng | 0.1 ng |
Maximum cDNA length |
20 kb | 20 kb | 13 kb | 13 kb |
Complete kits for efficient and reproducible first strand cDNA synthesis
| Enhanced performance | Routine performance | |||
|---|---|---|---|---|
| Product | Maxima H Minus First Strand cDNA Synthesis Master Mixes | Maxima First Strand cDNA Synthesis Kit | RevertAid H Minus First Strand cDNA Synthesis Kit | RevertAid First Strand cDNA Synthesis Kit |
| Active temperature range | 50–65 oC | 50–65 oC | 42–55 oC | 42–50 oC |
RNase H activity |
No | Yes | No | Yes |
| Reaction time | 15-30 min | 15-30 min | 60 min | 60 min |
| Formats with dsDNase* | Yes | Yes | N/A | N/A |
Sensitivity (total RNA) |
1 pg | 1 pg | 0.1 ng | 0.1 ng |
Maximum cDNA length |
20 kb | 20 kb | 13 kb | 13 kb |
| *dsDNase step is a quick (2 min) and easy gDNA removal step prior to RT step. | ||||
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For Research Use Only. Not for use in diagnostic procedures.
