Reverse transcription is a widely used application in molecular biology research for RNA and expression analysis. The Thermo Scientific reverse transcription portfolio offers robust and reliable reverse transcriptases in a variety of optimized formats for efficiency in RT-PCR and RT-qPCR applications. 

Most popular reverse transcription products

Maxima Reverse Transcriptase
An engineered enzyme developed through in vitro evolution of M-MuLV RT offering robust performance no conventional enzyme can match. Available as a stand-alone enzyme, cDNA synthesis kits and master mixes.

RevertAid First Strand cDNA Synthesis Kit
A complete system for efficient synthesis of first strand cDNA from RNA templates.

Verso 1-Step RT-PCR Kits
A highly processive reverse transcriptase enzyme stable up to 57°C with kits including regular or hot start Taq.

Featured Thermo Scientific reverse transcription product categories

Reverse Transcriptases

A range of reverse transcriptase (RT) enzymes, optimized to generate cDNA including RevertAid RT (a genetically modified M-MuLV) and Maxima RT (developed through in vitro evolution of M-MuLV).

cDNA Synthesis Kits

Convient kits for efficient and reproducible first strand cDNA synthesis including either RevertAid or Maxima Reverse Transcriptases (RT).

cDNA Synthesis Master Mixes

Complete one-tube solution supporting consistent and reliable cDNA synthesis for 2-step RT-qPCR with Maxima H Minus Reverse Transcriptase. Available with integrated gDNA removal protocol.

RT-PCR Kits

A complete set of RT-PCR reagents with a highly processive reverse transcriptase enzyme that is stable up to 57°C. Available with regular or hot start Taq.

RT-qPCR Kits

Kits developed to quantify RNA in a one step assay. Kits for both SYBR Green and probe chemistry are available offering a simple way to perform rapid, sensitive and reproducible RT-qPCR in a single tube.

Resources

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