cDNA Synthesis Kits—Thermo Scientific
Our selection of first strand cDNA synthesis kits includes either RevertAid or Maxima Reverse Transcriptases (RT). RevertAid is genetically modified M-MuLV RT and offers routine performance. Maxima RT was developed by in vitro evolution of M-MuLV enzyme for superior performance in cDNA synthesis.
Thermo Scientific™ cDNA synthesis kit selection table
|Routine performance||Enhanced performance|
|RevertAid H Minus First Strand cDNA Synthesis Kit||RevertAid First Strand cDNA Synthesis Kit||Maxima H Minus First Strand cDNA Synthesis Kit||Maxima First Strand cDNA Synthesis Kit for RT-qPCR|
|Optimal reaction temperature||42-45°C||42°C||50-55°C||50-55°C|
|Active up to||55°C||50°C||65°C||65°C|
|Reading length up to||13 kb||13 kb||20 kb||20 kb|
|RNase H activity||No||Yes||No||Yes|
|Formats with dsDNase*||Yes||Yes|
|Sensitivity (total RNA)||0.1 ng||0.1 ng||1 pg||1 pg|
|Reaction time||60 min||60 min||15-30 min||15-30 min|
*Allow quick (2 min) and easy gDNA removal prior to reverse transcription step
Additional cDNA synthesis kits
For Research Use Only. Not for use in diagnostic procedures.