Reverse Transcriptases—Thermo Scientific
We offer a range of reverse transcriptase (RT) enzymes, optimized to generate cDNA for a variety of research needs. RevertAid RT is genetically modified M-MuLV RT and offers routine performance. Maxima RT was developed by in vitro evolution of M-MuLV enzyme for superior performance in cDNA synthesis.
Thermo Scientific reverse transcriptase enzyme selection table
|Routine performance||Enhanced performance|
|RevertAid H Minus Reverse Transcriptase||RevertAid Reverse Transcriptase||Maxima H Minus Reverse Transcriptase||Maxima Reverse Transcriptase|
|Optimal reaction temperature||42-45°C||42°C||50-55°C||50-55°C|
|Active up to||55°C||50°C||65°C||65°C|
|Reading length up to||13 kb||13 kb||20 kb||20 kb|
|RNase H activity||No||Yes||No||Yes|
|Sensitivity (total RNA)||0.1 ng||0.1 ng||1 pg||1 pg|
|Reaction time||60 min||60 min||15-30 min||15-30 min|
Additional reverse transcriptase enzyme(s)
For Research Use Only. Not for use in diagnostic procedures.