Kits developed to quantify RNA in a one step assay. Kits for both SYBR Green and probe chemistry are available offering a simple way to perform rapid, sensitive and reproducible RT-qPCR in a single tube.

Thermo Scientific RT-qPCR kit selection table

  Probe Chemistry SYBR Green Chemistry
  Verso 1-Step RT-qPCR Kit Verso 1-Step RT-qPCR SYBR Green Kit
Optimal reaction temperature 50°C 50°C
Active up to 57°C 57°C
Blue dye - pipetting aid
Sensitivity (total RNA) 1 pg 1 pg
Master Mix without ROX
(separate ROX vial)
Yes
Master Mix with Low ROX Yes Yes
Master Mix with High ROX Yes
RT enhancer*

*Prevents gDNA carry-over

Resources

Find videos, webinars, articles, and tools in our Molecular biology resource library

Reverse transcription content