Kits, media, and reagents for the CTS Rotea system

Support for closed cell and gene therapy manufacturing from clinical translation through commercial manufacturing is offered by Rotea single-use kits and Gibco CTS off-the-shelf bagged media and reagents.

Single-use kits

The CTS Rotea closed system single-use kits offer flexibility for process development. Kits can be customized to support commercial scale manufacture. Closed, single-use kits enable multiple batches to be processed in a shared, grade C clean room leading to cost-effective transfer and scale-out.


Compatible with Luer connectors and sterile welding. Gamma sterilized.


  • Tube material: DEHP-free PVC
  • Standard kit: flow rates 5–110 mL/min
  • Hi-Flow kit: flow rates 30–160 mL/min

Closed-system compatible media and reagents

Cell therapy workflows are varied and complex. To meet the demand for media and reagents suitable for closed systems, the design of our bioprocess container is flexible and adaptable. 


Gibco CTS off-the-shelf bagged media support closed cell and gene therapy manufacturing from clinical translation through commercial manufacturing. Manufactured in state-of-the-art cGMP, ISO certified facilities to help ensure the highest quality and consistency for reproducible results.


Gibco CTS AIM-V Medium, without phenol red and without antibiotics, Gibco CTS DPBS, without calcium chloride and without magnesium chloride, Gibco CTS DPBS with calcium chloride and magnesium chloride, and Gibco CTS NK-Xpander Medium, can be used to scale up or scale out your manufacturing process. Each formulation is available in flexible bioprocess container format suitable for aseptic integration into your workflow through sterile welding, MPC quick-connect, or Luer lock connections.

Closed system compatible

You have the flexibility to sterile weld or aseptically connect to closed systems using a MPC quick connection or a female threaded Luer connection with injection site. Tubing polymers compatible with market-leading sterile welding equipment (including a section of PVC tubing that can be directly welded to the CTS Rotea Single Use kit) enable use in upstream and downstream applications.

Seamless translation

CTS reagents are now offered in closed packaging to help you reduce lead times, and scale and close your process to enable seamless clinical translation.

Quality testing and regulatory documentation

Reagents and single-use technologies comply with industry standards and are backed by regulatory documentation and support (Regulatory Support File, Certificate of Analysis, Certificate of Origin).

Compatible characterization instruments

Invitrogen Attune NxT Flow Cytometer 

Invitrogen Countess III Automated Cell Counter

QuantStudio 7 Pro Real-Time PCR System

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Caution: Not intended for direct administration into humans or animals.