Data from the Xenon Electroporation System demonstrates superior non-viral gene editing and transfection performance. Additional data will be added to this page as it becomes available.
In these data graphs, chimeric antigen receptor (CAR) T cells were generated from three different donors by using Cas9/gDNA to knock out the endogenous T-cell receptor (TCR) and knock in an FMC CAR. As a benchmark, lentiviral transduction would be expected to achieve 20–40% success for this process.
Knock-out and knock-in performance with the Xenon system was strong. Across all three donors, successful transfection percentages (cells both knocked out and knocked in) ranged from about 22% to more than 45%, depending on the electroporation chamber used. Transfection efficiency on the Xenon instrument surpassed even the Neon Transfection System on which the process was developed. Cell viability—which must be balanced against transfection efficiency—exceeded 70% in all but one case, and was usually within 10% of the untransfected controls. Percentages of CD4 and CD8 T cells remained relatively consistent before and after electroporation.
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