Mesenchymal stem cell media and reagents for cell therapy

Advancing your therapeutic mesenchymal stem cell therapy research to clinical applications requires careful material selection because the quality of starting materials significantly impact the properties of your final stem cell therapy product.

Gibco Cell Therapy Systems (CTS) products have been developed to ease the transition from stem cell therapy research to clinical applications by providing high quality GMP manufactured, commercial scale ancillary materials with a high degree of qualification, traceability and regulatory documentation. In an effort to help you maximize the potential of your adult stem cell therapy research and simplify the transition to clinic-ready processes we offer an extensive selection of research use mesenchymal stem cell research products with complementary CTS formulations for MSC clinical research and commercial applications.

CTS products are high quality and are a trusted source for MSC clinical research. Our CTS products are used in commercially approved cell therapies as well as over 200 clinical trials and are backed by our professional regulatory support and over 30 years of GMP manufacturing experience.

Gibco CTS Rotea Counterflow Centrifugation System

Gibco CTS Rotea Counterflow Centrifugation System

A compact, flexible closed cell processing system, designed to streamline and expedite your cell therapy development, that enables:

  • Process flexibility—user-programmable software enables you to create and optimize a broad range of protocols for cell separation, washing, and concentration
  • High cell recovery and viability—gentle processing enables >95% cell recovery while maintaining cell viability
  • Low output volumes—proprietary technology can deliver as little as 5 mL of concentrate
  • Research through commercial manufacturing—the closed single-use kit enables sterile processing, and an OPC-UA interface enables connectivity to a 21 CFR Part 11-compliant system

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Gibco CTS Xenon Electroporation System


A closed, scalable electroporation system for GMP-compliant cell therapy manufacturing

  • High speed and large volume—transfect up to 2.5 x 109 T cells/25 mL in less than 25 minutes
  • Scalable, proven performance and viability—up to 90% gene knockout with CTS TrueCut Cas9 Protein and 80% cell viability
  • Process flexibility—user-programmable system enables you to create and optimize electroporation protocols for various cell types and payloads, from process development through commercial manufacturing
  • Efficient nonviral transfection—can be used to deliver DNA, RNA, and protein payloads
  • Closed-system processing—Xenon MultiShot Electroporation Cartridge helps enable sterile welding to PVC or C-Flex™ tubing

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Mesenchymal stem cell therapy: workflow solutions

Mesenchymal stem cells (MSCs), also arguably known as stromal cells, are present in many tissues and are most known for their capacity to differentiate into specialized cells developing from mesoderm, namely osteoblasts, adipocytes and chondrocytes. Advancements in mesenchymal stem cell (MSC) research continue to shed light on how these multipotent stem cells may be used in various clinical applications. Preclinical and clinical adult mesenchymal stem cell therapy applications include immunomodulatory therapies (i.e., prevention of graft-versus-host disease or treatment of Crohn’s disease), autologous or allogeneic immunochemotherapies, and cell replacement therapies for mesenchymal tissues such as bone and cartilage. For more than a decade, we have provided key resources to address challenges in your stem cell workflow for your MSC clinical research. Designed to work together, our portfolio of mesenchymal stem cell products and services is designed to support and accelerate your path from discovery to the clinic.

Learn more about our MSC research portfolio

See more of our media and reagents

Translate your research to the clinic with Cell Therapy Systems products

To further help you maximize the potential of your research and streamline your transition to the clinic, we offer Gibco Cell Therapy Systems (CTS) equivalents for many of our research-use products. In addition to GMP manufacturing, CTS products undergo extensive safety testing and are accompanied by appropriate documentation so you can transition your cell therapy to the clinic with confidence.

  • Scalable manufacturing under GMP conditions—products manufactured  under 21 CFR Part 820 Quality System Regulation, USP 1043* and Ph Eur 5.2.12 at an ISO 13485-certified manufacturing site, with the ability to scale up and accommodate growing needs
  • Extensive safety testing and regulatory documentation—sterility, endotoxin, and testing as well as full traceability for raw materials, with Drug Master File available upon request
  • Designed for a seamless transition from research to clinic—serum-free, xeno-free or animal origin–free formulations with cell therapy specific intended use statements and complementary research use reagents

*Adherence to supplier related responsibilities of USP<1043>

Reagents for stem cell therapy research

See below for the extended list of reagents needed for stem cell maintenance and expansion. Gibco CTS GlutaMAX-1 is added to the Gibco CTS KnockOut SR XenoFree complete medium, and Gibco CTS TrypLE Select is an AOF reagent used for gentle cell dissociation.

CTS TrypLE Select

Gibco CTS TrypLE Select in bag and bottle format

CTS TrypLE Select, is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells. CTS TrypLE Select is derived from microbial fermentation and provides an animal origin–free (AOF) alternative for porcine trypsin.

  • Animal origin–free (AOF)—free of animal-derived components and formulated on dedicated animal origin-free equipment; CTS TrypLE Select may minimize the risk of viral contamination in your manufacturing process
  • Room temperature-stable—product remains stable at room temperature or 2–8°C, making storage and handling easy and convenient
  • Easy to use—CTS TrypLE Select substitutes directly into existing protocols with no inactivation required, avoiding the need for trypsin inhibitors, such as FBS

CTS DPBS, without calcium chloride, without magnesium chloride

Gibco CTS DPBS without calcium chloride, without magnesium chloride in bag and bottle format

Gibco Cell Therapy Systems CTS DPBS is the classical Dulbecco’s Phosphate Buffered Saline not containing calcium, magnesium, or phenol red. Gibco CTS DPBS, without calcium chloride, without magnesium chloride is now available in a flexible, closed system compatible packaging format.

  • Provides an environment that maintains the structural and physiological integrity of cells in vitro
  • Chemically defined and animal origin-free materials suitable for use in clinical research studies
  • Closed system compatible–flexibility for sterile welding, MPC quick connect, or Luer-Lock connections to integrate into your workflow

CTS GlutaMAX I, L-alanyl-L-glutamine

Gibco CTS GlutaMAX in bag and bottle format

CTS GlutaMAX supplement is an animal origin–free (AOF) dipeptide alternative to L-glutamine, with increased stability that improves cell health. CTS GlutaMAX supplement can be used as a direct substitute for L-glutamine atequimolar concentrations in mammalian and stem cell culture with minimal or no adaptation.

  • Minimizes toxic ammonia build-up—unlike L-glutamine, GlutaMAX Supplement does not spontaneously break down to form ammonia. This prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture
  • Improves growth efficiency and performance of mammalian cell cultures—lower levels of ammonia results in improved cell viability; in addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX supplement that is not possible with L-glutamine
  • Remains stable across a wide range of temperatures—CTS GlutaMAX supplement retains full functionality at room temperature or 2–8°C, for convenient storage and handling
Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.