Performance and application data for the Rotea system.

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Features


Performance data

Cell recovery and viability

Bar chart showing viability percentage and cell recovery percentage after T cell washing and concentration

Viability and recovery after T cell washing and concentration. Gentle processing enables >90% cell recovery while maintaining cell viability through T cell washing and concentration. High reproducibility was demonstrated over 10 runs.

Bar chart showing viability percentages before and after 200X T cell concentration

200X T cell concentration. The CTS Rotea system was used to concentrate 500 million T cells in a starting volume of 1,000 mL down to 5 mL achieving 97% cell recovery while maintaining viability of 94%.

Scatter plot showing % of cells recovered for PBMC from a Leukopak bag

High PBMC recovery from a Leukopak bag. The CTS Rotea sytem can achieve approximately 90% PBMC recovery from a Leukopak bag in combination with a red blood cell lysis buffer across multiple donors (n=7).

Cell concentration and output volumes

Bar chart showing viability and recovery percentages to highlight high concentration in low volumes (200X)


High concentration in low volumes (200X).
5 x 108 T cells concentrated into small volume.


Application data

Processing times

Input volume (L) Processing time (min) Cell recovery
T cell wash and concentrate (1x106 cells/mL)
0.5 13 >90%
1.0 18
3.0 50
5.0 116
10.0 230
Input volume (L) Processing time (min) Cell recovery
PBMC isolation
~0.25 (¼ Leukopak bag) 25  
~0.5 (½ Leukopak bag) 45
~1.0 (full Leukopak bag) 85
5 116
10 230

PBMC isolation with the CTS Rotea system vs Ficoll® media separation

Flow cytometry data shows that PBMC cell isolation with the the CTS Rotea instrument in less than 30 minutes produces similar results to a 2-hour Ficoll® media separation.

PBMC composition from CTS Rotea system vs manual Ficoll® polymer. A single-donor Leukopak was split in two and PBMCs were separated using the CTS Rotea System or a manual Ficoll media protocol. The PBMCs were assessed using successive gating to identify monocytes, B, NK, and T cells. Processing with the (A) CTS Rotea System took less than 30 minutes and produced equivalent results as (B) the 2-hour Ficoll media separation.

T cell quality and composition

Flow cytometry data shows that CD4/CD8 T cell composition within the CD3+ total T cell population remains largely unchanged after washing and concentration with the CTS Rotea instrument.

T cell quality and composition after washing and concentration. After PBMCs were isolated from healthy donors using the CTS Rotea System, the T cells were expanded in culture and assessed for CD4 and CD8 markers using flow cytometry. They were then washed and concentrated using the CTS Rotea System and assessed again. Comparison showed that CD4 and CD8 populations were consistent between the two analyses, demonstrating that the relative proportions were unaffected by CTS Rotea system processing.