Expand your molecular research offerings with infectious disease panels

 

A flexible, scalable, and reliable solution for lab optimization utilizing real-time PCR instrumentation

With the unpredictable, rapidly changing nature of SAR-CoV-2, labs are expanding their test menus to accommodate a growing number of infectious disease areas and finding ways to improve the precision and accuracy in their research areas. Detection of common infectious diseases with traditional culture- or microscopy-based methods are antiquated and lack sensitivity and specificity. Real-time PCR (qPCR) is an innovative solution for detecting slow-growing, difficult-to-cultivate, or uncultivable microorganisms.

In order to effectively study outbreaks and determine disease etiology where pathogens are similar, analytically sensitive panels are needed to support laboratory research studies.

Applied Biosystems TrueMark Infectious Disease Research Panels are convenient, duplexed TaqMan assays that are performed on pre-spotted and dried-down plates that include microorganism‑specific pathogens, utilizing real‑time PCR techniques.

This enables rapid, accurate detection and categorization of microorganisms that cause respiratory, vaginal, urinary, gastrointestinal, and sexually transmitted diseases, as well as antibiotic resistance research.

TrueMark Infectious Disease Research Solutions are available in customizable options. Choose from an extensive list of pathogens to put on your plate, or ten different predefined panels in several disease states, with preselected assays targets.


Key features

Benefits of the TrueMark Panels

  • Customizable—choose from a selection of predefined panels or customer-defined panels
  • Ease-of-use—convenient, duplexed reactions include assay and positive control, pre-spotted and dried down on TaqMan plates, designed for easy set-up and increased accuracy; just add sample and approved TaqPath Master Mix
  • Expanded content—pathogen detection panels available for researching respiratory, urinary, gastrointestinal, vaginal, and sexually transmitted infections, as well as antibiotic resistance assays
  • Internal process controls—duplex reactions offer RNase P human internal control or Bacillus atrophaeus bacterial spike-in control* in every well for added confidence
  • Amplification control—TrueMark Amplification Control I covers all targets in RTI, UTI, STI, and vaginal panels

Benefits of molecular testing

  • Increased sensitivity—more specific, accurate, and precise compared to traditional culture and microscopy methods
  • Timely results—results from PCR testing are faster than traditional methods, which are manual

Benefits of buying from Thermo Fisher Scientific

  • Complete end-to-end solution—optimized for use on QuantStudio qPCR systems with verified sample-to-answer workflow
  • Expertise in assay design—run comprehensive molecular testing panels with assays optimized for maximal strain coverage, sensitivity, and internal control

For Research Use Only. Not for use in diagnostic procedures.

Scientist conducting qPCR panel testing

Increased productivity, sensitivity, and specificity with qPCR technology

Implementing genetic analysis systems and solutions in the laboratory has been directly linked to improved efficiencies, cost savings, and faster time to reportable results.** qPCR can detect slow-growing, difficult-to-cultivate microorganisms, making it ideal for when culture methods are inadequate, ambiguous, time-consuming, difficult, or costly.

Learn more about implementing molecular testing with qPCR in your lab ›

 

Maximize your workflow

Expand from SARS-CoV-2 testing to TrueMark Infectious Disease Research Panels utilizing the same workflow, with our custom, made-to-order, or predefined panels on TaqMan Array Plates for 96-well and 384-well panels which are compatible for use on QuantStudio qPCR systems. Go from sample to result in approximately four hours.

New to Thermo Fisher molecular testing?  Easily click on the steps to below to see the recommended components needed to set up your laboratory for real-time PCR testing.


  • Vaginal swab, genital swab, lesion swab, nasopharyngeal swab, nasopharyngeal aspirate, or urine sample***

 

Use existing workflow including KingFisher systems and MagMAX kits

  • KingFisher Flex Purification System
  • KingFisher Apex Purification System
  • Applied Biosystems MagMAX Ultra
    Nucleic Acid Isolation Kits
  • TrueMark Comprehensive Microbiota Control

  • Select panel for either 96- or 384-well block plate, and add sample and Applied Biosystems multiplex master into wells
  • Applied Biosystems QuantStudio 5 Real
    -Time PCR System
  • Applied Biosystems QuantStudio 7 Flex
    Real-Time PCR System
  • Applied Biosystems QuantStudio 7 Pro Real-
    Time PCR System
  • Applied Biosystems QuantStudio 12K Flex
    Real-Time PCR System

 QuantStudio 5 Dx and QuantStudio 7 Pro Dx systems are For In Vitro Diagnostic Use. Test development mode is for Research Use Only. Not for use in diagnostic procedures.

  • Applied Biosystems QuantStudio Design and Analysis Software


  • Vaginal swab, genital swab, lesion swab, nasopharyngeal swab, nasopharyngeal aspirate, or urine sample***

 

Use existing workflow including KingFisher systems and MagMAX kits

  • KingFisher Flex Purification System
  • KingFisher Apex Purification System
  • Applied Biosystems MagMAX Ultra
    Nucleic Acid Isolation Kits
  • TrueMark Comprehensive Microbiota Control

  • Select panel for either 96- or 384-well block plate, and add sample and Applied Biosystems multiplex master into wells
  • Applied Biosystems QuantStudio 5 Real
    -Time PCR System
  • Applied Biosystems QuantStudio 7 Flex
    Real-Time PCR System
  • Applied Biosystems QuantStudio 7 Pro Real-
    Time PCR System
  • Applied Biosystems QuantStudio 12K Flex
    Real-Time PCR System

 QuantStudio 5 Dx and QuantStudio 7 Pro Dx systems are For In Vitro Diagnostic Use. Test development mode is for Research Use Only. Not for use in diagnostic procedures.

  • Applied Biosystems QuantStudio Design and Analysis Software


Additional benefits of TrueMark Panels

TrueMark Infectious Disease Research Panels are a collection of duplex assays designed to detect a pathogen of interest and an internal control in the same reaction. Customers have the option of predefined panels covering 8 pathogens per panel on 96-well plates or custom panel options on either 96-or 384-well plates.

Duplexed assays 

  • Allow for the simultaneous detection of target and control in the same well
  • Help increase confidence in results
  • Are designed to reduce the need to run replicates, helping to lower cost and minimize workflow complexity

Pre-spotted and dried down plates

  • Target and RNAse P or Bacillus atrophaeus process control assays dried down in plate 
  • Designed to reduce hands on time and errors in the workflow

Amplification control

  • One control covers all disease area targets, panels, and applications
  • Makes it easier and faster for labs to implement


TrueMark Predefined Plus Panel options

Choose from ten different predefined panels in several disease areas, covering 8 pathogens each. Pathogens included in predefined options are to be used for research purposes only.

Table 1. Applied Biosystems TrueMark Respiratory I Plus Panel

Pathogen Type

Species

Sample Type

Viral

Adenovirus
Enterovirus
Influenza A (Pan) Influenza B
Respiratory Syncytial Virus A/B (RSV A/B)
Rhinovirus
Human Metapneumovirus (HM)

SARS-CoV-2 ORF1ab

 

 

Nasopharyngeal swab or

nasopharyngeal aspirate***

Control Type

RNase P internal control

Table 2. Applied Biosystems TrueMark Respiratory II Plus Panel

Pathogen Type

Species

Sample Type

Viral

Coronavirus 229E (CE)
Coronavirus HKU1 (CH)
Coronavirus NL63 (CN)
Coronavirus OC43 (CO)
Parainfluenza 1 (P1)
Parainfluenza 2 (P2)
Parainfluenza 3 (P3)
Parainfluenza 4 (P4)

 

 

Nasopharyngeal swab or

nasopharyngeal aspirate***

Control Type

RNase P internal control

Table 3. Applied Biosystems TrueMark Respiratory III Plus Panel

Pathogen Type

Species

Sample Type

Bacterial

C. pneumoniae
Moraxella catarrhalis
Klebsiella pneumonia
Staphylococcus aureus (SA)
Haemophilus Influenzae (HI)
Legionella pneumophila (LP)
Mycoplasma pneumoniae (MP)
Streptococcus pneumoniae (SP)

 

 

Nasopharyngeal swab or

nasopharyngeal aspirate***

Control Type

RNase P internal control

 

Table 4. Applied Biosystems TrueMark Urinary I Plus Panel

Pathogen Type

Species

Sample Type

Bacterial

Pseudomonas aeruginosa
Proteus mirabilis
Escherichia coli
Klebsiella pneumoniae
Staphylococcus aureus
Coagulase negative staphylococci
Staphylococcus saprophyticus

 

 

 

Urine sample***

Fungal

Candida albicans

Control Type

Bacillus Atrophaeus spike-in control*

 

Table 5. Applied Biosystems TrueMark Urinary II Plus Panel

Pathogen Type

Species

Sample Type

Bacterial

Corynebacterium riegelii
Enterobacter aerogenes
Aerococcus urinae
Proteus vulgaris
Acinetobacter baumannii
Actinobaculum schaalii
Enterococcus faecalis
Streptococcus agalactiae

 

 

Urine sample***

Control Type

Bacillus Atrophaeus spike-in control*

Table 6. Applied Biosystems TrueMark Urinary III Plus Panel

Pathogen Type

Species

Sample Type

Bacterial

Citrobacter freundii
Enterobacter cloacae
Enterococcus faecium
Klebsiella oxytoca
Serratia marcescens
Mycoplasma hominis
Morganella morganii
Providencia stuartii

 

 

Urine sample***

Control Type

Bacillus Atrophaeus spike-in control*

Table 7. Applied Biosystems TrueMark STI Plus Panel

Pathogen Type

Species

Sample Type

Bacterial

Chlamydia trachomatis (CT)
Neisseria gonorrhoeae (NG)
Mycoplasma genitalium (MG)
Mycoplasma hominis (MH)
Ureaplasma parvum (UP)
Ureaplasma urealyticum (UU)
Herpes simplex virus type 1 & 2 (HSV1 + HSV2)

 

 

Vaginal swab or

genital swab***

Parasite

Trichomonas vaginalis (TV)

Control Type

RNase P internal control

Table 8. Applied Biosystems TrueMark Vaginal Plus Panel

Pathogen Type

Species

Sample Type

Bacterial

Gardnerella vaginalis (GV)
Atopobium vaginae (AV)
Prevotella bivia
Mobiluncus curtisii (MC)
Bacteroides fragilis
Pan–Lactobacillus
Megasphaera Type 1 (Mega1)
Bacterial vaginosis–associated bacteria 2 (BVAB2)

 

 

Vaginal swab***

Control Type

RNase P internal control

Table 9. Applied Biosystems TrueMark Lesion Plus Panel

Pathogen Type

Species

Sample Type

Bacterial

Haemophilus ducreyi (HD)
Treponema pallidum (TP)
Lymphogranuloma Venereum (LGV)

 

 

 

Lesion swab***

Viral

Herpes simplex virus type 1 (HSV1)
Herpes simplex virus type 2 (HSV2)
Cytomegalovirus (CMV)
Varicella-zoster virus (VZV)

Fungal

Pan-Candida

 

Control Type

RNase P internal control

Table 10. Applied Biosystems TrueMark Genital Plus Panel

Pathogen Type

Species

Sample Type

Bacterial

Gardnerella vaginalis (GV)

 

 

 

 

Vaginal swab or

genital swab***

Fungal

Candida albicans
Candida krusei
Candida dubliniensis
Candida glabrata
Candida tropicalis
Candida parapsilosis

Parasite

Trichomonas vaginalis (TV)

 

Control Type

RNase P internal control

Custom assay design

Can’t find what you need from our portfolio of predefined assays?  Please browse through our portfolio of microbe assays and resistance gene assays.


Ordering information


TrueMark Infectious Disease Predefined Plus Panel offerings

Combo Kit components

Catalog #

Name

Size

Included in Combo Kit

TrueMark Infectious Disease 1-Step Multiplex Master Mix (No ROX)

3 X 1 mL

 

TrueMark Infectious Disease Custom Panel offerings

Custom Plate Options††

Description

Cat. No. ††

TrueMark Respiratory Panel

Contact sales representative ›

TrueMark Urinary Panel

Contact sales representative ›

TrueMark UTI and Vaginal Panel

Contact sales representative ›

TrueMark Gastrointestinal Panel

Contact sales representative ›

TrueMark Antibiotic Resistance Panel

Contact sales representative ›

TrueMark Custom Assay Panel

Contact sales representative ›

 

For use with TrueMark Infectious Disease Custom Panels, sold separately

Additional components needs for both TrueMark Infectious Disease Predefined and Custom Panels, sold separately


*Spike in the Applied Biosystems TaqMan Universal Extraction Control Organism (Cat. No. A39180) containing Bacillus atrophaeus for use as a process control.
** Davenport et al. New and developing diagnostic technologies for urinary tract infections. Nat Rev Urol. (2017) 14: 296–310.
***Internally tested sample types. Laboratories can validate additional sample types.
† Combo Kits include 5 TaqMan Array plates and 3 x 1 mL tubes of TrueMark Infectious Disease Multiplex Master Mix (No ROX)
†† For specific panel options that are available with each Cat. No., contact your sales representative.

For Research Use Only. Not for use in diagnostic procedures.

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