Expand your molecular research offerings with infectious disease panels
A flexible, scalable, and reliable solution for lab optimization utilizing real-time PCR instrumentation
With the unpredictable, rapidly changing nature of SAR-CoV-2, labs are expanding their test menus to accommodate a growing number of infectious disease areas and finding ways to improve the precision and accuracy in their research technology. Detection of common infectious diseases with traditional culture- or microscopy-based methods are antiquated and lack sensitivity and specificity. Real-time PCR (qPCR) is an innovative solution for detecting slow-growing, difficult-to-cultivate, or uncultivable microorganisms.
Applied Biosystems TrueMark Infectious Disease Research Panels are convenient, duplexed TaqMan assays that are performed on pre-spotted and dried-down plates that include microorganism‑specific pathogens, utilizing real‑time PCR techniques.
This enables rapid, accurate detection and categorization of microorganisms that cause respiratory, vaginal, urinary, gastrointestinal, and sexually transmitted diseases, as well as antibiotic resistance research.
Key features
Benefits of the TrueMark Panels
- Customizable—choose from a selection of predefined panels or customer-defined panels
- Ease-of-use—convenient, duplexed reactions include assay and positive control, pre-spotted and dried down on TaqMan plates, designed for easy set-up and increased accuracy; just add sample and approved TaqPath Master Mix
- Expanded content—pathogen detection panels available for researching respiratory, urinary, gastrointestinal, vaginal, and sexually transmitted infections, as well as antibiotic resistance assays
- Endogenous controls—duplex reactions offer RNase P human internal control or Bacillus atrophaeus bacterial spike-in control* in every well for added confidence
- Amplification control—TaqMan Comprehensive Microbiota Control covers all targets in RTI, UTI, STI, and vaginal panels
Benefits of molecular testing
- Increased sensitivity—more specific, accurate, and precise compared to traditional culture and microscopy methods
- Timely results—results from PCR testing are more accurate than traditional methods, which are manual
Benefits of buying from Thermo Fisher Scientific
- Complete end-to-end solution—optimized for use on QuantStudio qPCR systems with verified sample-to-answer workflow
- Expertise in assay design—run comprehensive molecular testing panels with assays optimized for maximal strain coverage, sensitivity, and internal control
For Research Use Only. Not for use in diagnostic procedures.
Maximize your workflow
Expand from SARS-CoV-2 testing to TrueMark Infectious Disease Research Panels utilizing the same workflow, with our custom, made-to-order, or predefined panels on TaqMan Array Plates for 96-well and 384-well panels which are compatible for use on QuantStudio qPCR systems. Go from sample to result in approximately four hours.
- Vaginal swab, nasopharyngeal swab, or urine sample
- Use existing workflow including KingFisher systems and MagMAX kits
- Select panel for either 96- or 384-well block plate, and add sample and Applied Biosystems multiplex master into wells
- Load plate into existing instrument and run PCR
- Applied Biosystems QuantStudio Design and Analysis Software
- Vaginal swab, nasopharyngeal swab, or urine sample
- Use existing workflow including KingFisher systems and MagMAX kits
- Select panel for either 96- or 384-well block plate, and add sample and Applied Biosystems multiplex master into wells
- Load plate into existing instrument and run PCR
- Applied Biosystems QuantStudio Design and Analysis Software
Additional benefits of TrueMark Panels
TrueMark Infectious Disease Research Panels are a collection of duplex assays that detects microbes and internal controls in the same reaction. Customers have the option of predefined panels on 96-well plates or custom assay options on either 96-or 384-well plates.
Duplexed assays
- Allow for the simultaneous detection of target and control in the same well
- Help increase confidence in results
- Are designed to reduce the need to run replicates, helping to lower cost and minimize workflow complexity
Pre-spotted and dried down plates
- Target and RNAse P process control assays dried down in plate
- Designed to reduce hands on time and errors in the workflow
Amplification control
- One control covers all disease area targets, panels, and applications
- Makes it easier and faster for labs to implement
Table 1. Applied Biosystems TrueMark Respiratory I Plus Panel
| Pathogen Type | Species | Sample Type |
| Adenovirus Enterovirus Influenza A (Pan) Influenza B Respiratory Syncytial Virus A/B (RSV A/B) Rhinovirus Human Metapneumovirus (HM) SARS-CoV-2 ORF1ab |
Nasopharyngeal swab | |
Control Type | RNase P internal control |
Table 2. Applied Biosystems TrueMark Respiratory II Plus Panel
Pathogen Type | Species | Sample Type |
Viral | Coronavirus 229E (CE) Coronavirus HKU1 (CH) Coronavirus NL63 (CN) Coronavirus OC43 (CO) Parainfluenza 1 (P1) Parainfluenza 2 (P2) Parainfluenza 3 (P3) Parainfluenza 4 (P4) |
Nasopharyngeal swab |
Control Type | RNase P internal control |
Table 3. Applied Biosystems TrueMark Respiratory III Plus Panel
Pathogen Type | Species | Sample Type |
Bacterial | C. pneumoniae Moraxella catarrhalis Klebsiella pneumonia Staphylococcus aureus (SA) Haemophilus Influenzae (HI) Legionella pneumophila (LP) Mycoplasma pneumoniae (MP) Streptococcus pneumoniae (SP) |
Nasopharyngeal swab |
Control Type | RNase P internal control |
Table 4. Applied Biosystems TrueMark Urinary I Plus Panel
Pathogen Type | Species | Sample Type |
Bacterial | Pseudomonas aeruginosa Streptococcus agalactiae Proteus mirabilis Enterococcus faecalis Escherichia coli Klebsiella pneumoniae Staphylococcus aureus |
Urine sample |
Fungal | Candida albicans | |
Control Type | Bacillus Atrophaeus spike-in control* |
Table 5. Applied Biosystems TrueMark Urinary II Plus Panel
Pathogen Type | Species | Sample Type |
Bacterial | Corynebacterium riegelii Enterobacter aerogenes Aerococcus urinae Proteus vulgaris Acinetobacter baumannii Coagulase negative Staphylococci Actinobaculum schaalii Staphylococcus saprophyticus |
Urine sample |
Control Type | Bacillus Atrophaeus spike-in control* |
Table 6. Applied Biosystems TrueMark Urinary III Plus Panel
Pathogen Type | Species | Sample Type |
Bacterial | Citrobacter freundii Enterobacter cloacae Enterococcus faecium Klebsiella oxytoca Serratia marcescens Mycoplasma hominis Morganella morganii Providencia stuartii |
Urine sample |
Control Type | Bacillus Atrophaeus spike-in control* |
Table 7. Applied Biosystems TrueMark STI Plus Panel
Pathogen Type | Species | Sample Type |
Bacterial | Chlamydia trachomatis (CT) Neisseria gonorrhoeae (NG) Mycoplasma genitalium (MG) Mycoplasma hominis (MH) Ureaplasma parvum (UP) Ureaplasma urealyticum (UU) Herpes simplex virus type 1 & 2 (HSV1 + HSV2) |
Vaginal swab |
Parasite | Trichomonas vaginalis (TV) | |
Control Type | RNase P internal control |
Table 8. Applied Biosystems TrueMark Vaginal Plus Panel
Pathogen Type | Species | Sample Type |
Bacterial | Gardnerella vaginalis (GV) Atopobium vaginae (AV) Prevotella bivia Mobiluncus curtisii (MC) Bacteroides fragilis Pan–Lactobacillus Megasphaera Type 1 (Mega1) Bacterial vaginosis–associated bacteria 2 (BVAB2) |
Vaginal swab |
Control Type | RNase P internal control |
Table 9. Applied Biosystems TrueMark Lesion Plus Panel
Pathogen Type | Species | Sample Type |
Bacterial | Haemophilus ducreyi (HD) |
Vaginal swab |
Viral | Herpes simplex virus type 1 (HSV1) Herpes simplex virus type 2 (HSV2) Cytomegalovirus (CMV) Varicella-zoster virus (VZV) | |
Fungal | Pan-Candida
| |
Control Type | RNase P internal control |
Table 10. Applied Biosystems TrueMark Genital Plus Panel
Pathogen Type | Species | Sample Type |
Bacterial | Gardnerella vaginalis (GV)
|
Vaginal swab |
Fungal | Candida albicans Candida krusei Candida dubliniensis Candida glabrata Candida tropicalis Candida parapsilosis | |
Parasite | Trichomonas vaginalis (TV)
| |
Control Type | RNase P internal control |
Custom assay design
Can’t find what you need from our portfolio of predefined assays? Use our TaqMan Custom Design Assay Tool to easily design your own research assay based on your target sequence of interest or submit the primer/probe sequences of your own design. Each custom assay is a mix of forward primer, reverse primer, and FAM and/or VIC dye-labeled TaqMan MGB probe.
Increased productivity, sensitivity, and specificity with qPCR technology
Implementing genetic analysis systems and solutions in the laboratory has been directly linked to improved efficiencies, cost savings, and faster time to reportable results.** qPCR can detect slow-growing, difficult-to-cultivate microorganisms, making it ideal for when culture methods are inadequate, ambiguous, time-consuming, difficult, or costly.
Ordering information
*Spike in the Applied Biosystems TaqMan Universal Extraction Control Organism (Cat. No. A39180) containing Bacillus atrophaeus for use as a process control.
** Davenport et al. (2017) New and developing diagnostic technologies for urinary tract infections. Nat Rev Urol. volume 14, pages 296–310.
***Control needs to be manually pipetted into each well
† Available in predefined or custom panels.
‡ Available only as custom panel option; contact your sales representative or customer service for more information.
Resources
For Research Use Only. Not for use in diagnostic procedures.
