Partner with Thermo Fisher Scientific for a full-scale workflow solution to rapidly detect and help contain the monkeypox virus
 

What is monkeypox virus (MPX or Mpox)?

  • Monkeypox is a DNA virus endemic to Central and West African countries.
  • Monkeypox is an orthopoxvirus, the genus of viruses that cause diseases like smallpox. It is associated with skin lesions and lymph node swelling among other symptoms.
  • Tropical rainforest animals including rodents and non-human primates are the primary carriers of monkeypox.
  • There are two distinct clades of MPXV—a West African clade and a Congo Basin clade. People infected by virus from the Congo Basin/Central African clade experience approximately 10% case fatality rate while those infected by the West African clade experience a <4% case fatality rate.1

With our reliable supply chain already helping to meet the unprecedented demand for trusted COVID-19 testing, we have continued to expand our high-quality portfolio of Public Health outbreak solutions and accelerated innovation with urgency.

1World Health Organization (WHO). Multi-country monkeypox outbreak in non-endemic countries. 21 May 2022.


Features and benefits
 

TaqPath Monkeypox/Orthopox Virus DNA Kit features include:

  • Multiplex kitted solution (assay plus control)
  • Simultaneous detection of Monkeypox virus, non-variola Orthopoxvirus, and human RNase P
  • EUA-authorized validated kit
  • Lesion swab sample type
  • Endogenous RNase P control
  • High specificity and sensitivity

Focus

For low-high throughput applications

Targets

Monkeypox virus (hMPXV)

Non-variola Orthopoxvirus (OPXV)

Human RNase P

Sample type

Skin lesion specimen

Format

Multiplex (3-plex) in single well

Compatible real-time PCR instruments

QuantStudio 5 (96-well, 0.1 mL well)
QuantStudio 7 Flex (384-well)
QuantStudio 5 Dx (96-well, 0.2 mL)

Controls

Amplification control


Workflow
 

Sterile swab sample collection
  • Lesion swab specimens and transport media
  • Viral transport media (VTM)
  • Universal transport media (UTM)
DNA extraction kit
Thermo Scientific KingFisher Flex Purification System
  • Automated processing with the Thermo Scientific KingFisher Flex Purification System with 96 deep-well head and KingFisher Flex 96 Heating Block
  • Applied Biosystems MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit
  • Applied Biosystems TaqPath Monkeypox/Orthopox Virus DNA Kit
  • Applied Biosystems TaqPath BactoPure Microbial Detection Master Mix (No ROX)
Applied Biosystems Quanstudio Real-Time PCR Systems compatible with the monkeypox/orthovirus kit
  • Applied Biosystems QuantStudio 5 Real-Time PCR system (96-well, 0.1 mL block)
  • Applied Biosystems QuantStudio 5 Dx Real-Time PCR system (96-well, 0.2 mL block)
  • Applied Biosystems QuantStudio 7 Flex Real Time PCR system (384-well block) with QuantStudio Real-Time Software
Applied Biosystems Pathogen Interpretive Software
  • Applied Biosystems Pathogen Interpretive Software
Sterile swab sample collection
  • Lesion swab specimens and transport media
  • Viral transport media (VTM)
  • Universal transport media (UTM)
DNA extraction kit
Thermo Scientific KingFisher Flex Purification System
  • Automated processing with the Thermo Scientific KingFisher Flex Purification System with 96 deep-well head and KingFisher Flex 96 Heating Block
  • Applied Biosystems MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit
  • Applied Biosystems TaqPath Monkeypox/Orthopox Virus DNA Kit
  • Applied Biosystems TaqPath BactoPure Microbial Detection Master Mix (No ROX)
Applied Biosystems Quanstudio Real-Time PCR Systems compatible with the monkeypox/orthovirus kit
  • Applied Biosystems QuantStudio 5 Real-Time PCR system (96-well, 0.1 mL block)
  • Applied Biosystems QuantStudio 5 Dx Real-Time PCR system (96-well, 0.2 mL block)
  • Applied Biosystems QuantStudio 7 Flex Real Time PCR system (384-well block) with QuantStudio Real-Time Software
Applied Biosystems Pathogen Interpretive Software
  • Applied Biosystems Pathogen Interpretive Software


Ordering information
 

Style Sheet for Global Design System