Target multiple respiratory pathogens on a single panel

 

Applied Biosystems TrueMark Respiratory Panels are sensitive, real-time PCR syndromic panels for the detection of a wide range of respiratory pathogens including viruses, bacteria, and fungi that often present as symptomatically similar. Our solutions accurately detect more pathogenic organism targets than any other currently available test and at a lower cost per sample, helping enable your lab to deliver more informative results while achieving greater efficiency. These solutions also solve many of the challenges associated with immunoassays, culture-based, and even other molecular methods available today.  It is a leap forward in effectively studying outbreaks, determining disease etiology where symptoms are similar and, of critical importance, identifying co-infections to clarify treatment options.

Broad pathogen detection—Our solutions detect a broad range of viral, bacterial, and fungal targets in one panel—the most available in a single panel on the market today

Flexible input types—Multiple sample input types can be used, including nasopharyngeal (NP) swabs, bronchoalveolar lavage (BAL), and nasal aspirate

Highly accurate—Enable maximum strain coverage while minimizing off-target cross-reactivity

Scalable and efficient—Our variety of real-time PCR assay formats enable you to process tens to hundreds of samples per day on a single instrument

Analytical validation—Service available to accelerate the implementation and testing of menu expansion


Your qPCR instrument can help to expand your respiratory detection beyond COVID-19
 

Polymicrobial detection

Real-time PCR can detect slow-growing or difficult-to-culture microorganisms and can be used when traditional methods lead to ambiguous results. Our TrueMark Respiratory Panel solution is available in OpenArray Plates, TaqMan Array Cards, and TaqMan Array Plates, and can be used with multiple sample input types, such as nasopharyngeal (NP) swabs, bronchoalveolar lavage (BAL), and nasal aspirate.

Utilizing TaqMan Assays enable rapid, accurate detection and categorization of multiple microorganisms involved in respiratory tract microbiome composition and dynamics. Each TaqMan Array Card can detect up to 41 known pathogens, while the OpenArray (OA) Plates can detect up to 33 pathogens with the ability to run up to 24 samples per plate.  Both formats are available in two different versions—with or without SARS-CoV 1 & 2 targets—depending on your detection needs.

TrueMark respiratory tract infection testing menu

43
Targets available

12
Bacteria

1
Fungus

30
Viruses

 


Viruses 
Adenovirus
Bocavirus
Coronavirus 229E
Coronavirus HKU1
Coronavirus NL63
Coronavirus OC43
Enterovirus (Pan)
Enterovirus D68
Herpesvirus 6 (HHV6)
HHV3 (varicella zoster virus)
HHV4 (Epstein-Barr virus)
HHV5 (cytomegalovirus)
Metapneumovirus (hMPV)
Influenza A (Pan)
Influenza A/H1-2009
Influenza A/H3
Influenza B
Measles virus
MERS-CoV
Mumps virus
Parainfluenza virus 1
Parainfluenza virus 2
Parainfluenza virus 3
Parainfluenza virus 4
Parechovirus
Respiratory Syncytial Virus A (RSV A)
Rhinovirus
Respiratory Syncytial Virus B (RSV B)
SARS-CoV (SARS)**
SARS-CoV-2**

Fungus 
P. jirovecii


Bacteria
Bordetella (Pan)
Bordetella pertussis
Bordatella holmesii*
Chlamydophila pneumoniae
Coxiella burnetta*
Haemophilus influenzae
Klebsiella pneumoniae complex
Legionella pneumophila
Moraxella catarrhalis
Mycoplasma pneumoniae
Staphylococcus aureus
Streptococcus pneumoniae


*Only available on TaqMan™ Array Respiratory Tract Microbiota Comprehensive Card
**Only available on TrueMark™ Respiratory Panel 2.0, TaqMan™ Array card

 

Pathogen Type

TaqMan Card

TaqMan Card 2.0

Bacterial

S. aureus

S. aureus

K. pneumoniae

K. pneumoniae

C. pneumoniae

C. pneumoniae

L. pneumophila

L. pneumophila

C. burnetii

 

S. pneumoniae

S. pneumoniae

M. pneumoniae

M. pneumoniae

B. holmesii

 

M. catarrhalis

M. catarrhalis

B. pertussis

B. pertussis

Bordetella

Bordetella

H. influenzae

H. influenzae

B.atrophaeus

B. atrophaeus

Fungal

P. jirovecii

P. jirovecii

Viral

HHV6

HHV6

EV_pan

EV_pan

SARS_CoV

SARS_CoV

hPIV1

hPIV1

HHV5

HHV5

MERS_CoV

MERS_CoV

CoV_OC43

CoV_OC43

HHV3

HHV3

Mumps

Mumps

EV_D68

EV_D68

hPIV3

hPIV3

CoV_229E

CoV_229E

hPIV2

hPIV2

CoV_NL63

CoV_NL63

CoV_HKU1

CoV_HKU1

HHV4

HHV4

AdV_1of2

AdV_1of2

AdV_2of2

AdV_2of2

HBoV

HBoV

hMPV

hMPV

hPIV4

hPIV4

HPeV

HPeV

Flu_A_H1

Flu_A_H1

Flu_A_H3

Flu_A_H3

Flu_A_pan

Flu_A_pan

Flu_B_pan

Flu_B_pan

Measles

Measles

RSVA

RSVA

RSVB

RSVB

RV_1of2

RV_1of2

RV_2of2

RV_2of2

 

SARS-CoV2S

 

SARS-CoV2N

Control

Xeno

Xeno

RPPH1

RPPH1

18s

18s

Pathogen Type

OpenArray Plate

OpenArray Plate 2.0

Bacterial

S. aureus

S. aureus

K. pneumoniae

K. pneumoniae

C. pneumoniae

C. pneumoniae

L. pneumophila

L. pneumophila

S. pneumoniae

S. pneumoniae

M. pneumoniae

M. pneumoniae

B. pertussis

B. pertussis

Bordetella

Bordetella

H. influenzae

H. influenzae

Viral

HHV6

HHV6

EV_pan

EV_pan

hPIV1

hPIV1

HHV5

HHV5

CoV_OC43

CoV_OC43

HHV3

 

EV_D68

EV_D68

hPIV3

hPIV3

CoV_229E

CoV_229E

hPIV2

hPIV2

CoV_NL63

CoV_NL63

CoV_HKU1

CoV_HKU1

HHV4

HHV4

AdV_1of2

AdV_1of2

AdV_2of2

AdV_2of2

HBoV

 

hMPV

hMPV

hPIV4

hPIV4

Flu_A_H1

Flu_A_H1

Flu_A_H3

Flu_A_H3

Flu_A_pan

Flu_A_pan

Flu_B_pan

Flu_B_pan

RSVA

RSVA

RSVB

RSVB

RV_1of2

RV_1of2

RV_2of2

RV_2of2

 

SARS-CoV2S

 

SARS-CoV2N

Control

IC Xeno

IC Xeno

IC B.atrophaeus

IC B.atrophaeus

IC RNase P

IC RNase P

To download a PDF version of the information above, please visit Pathogen Target Guide for Respiratory Panels.

Highly accurate

TaqMan Assays for respiratory pathogens have undergone rigorous bioinformatic analysis to help ensure maximum strain coverage while minimizing off-target cross-reactivity. Each assay has been tested with target and non-target genomic RNA or DNA isolated from target organisms (nucleic acid acquired from ATCC) in our inclusivity panel.

Pathogen

True positive*

False negative**

Positive percent agreement

Adenovirus

20

1

95.24%

Coronavirus 229E

2

0

100.00%

Coronavirus HKU1

1

0

100.00%

Coronavirus OC34

1

0

100.00%

Influenza A

4

0

100.00%

Influenza A/H1

6

0

100.00%

Influenza A/H3

16

0

100.00%

Influenza B

16

0

100.00%

hMPV

16

0

94.12%

hPIV1

10

1

90.91%

hPIV2

10

0

100.00%

hPIV3

12

0

100.00%

RSV

6

0

100.00%

RSVA

17

1

94.44%

RSVB

16

1

100.00%

Rhinovirus, enterovirus

27

0

100.00%

Total

180

5

97.30%

* Call from the vendor

** Call not detected when tested on TaqMan Array Card Assay (TAC)

The sensitivity, specificity, and accuracy of the assays on the TaqMan Array Respiratory Tract Microbiota Comprehensive Card were examined by testing with 180 purchased clinical research samples that were previously characterized for respiratory pathogens by other molecular methods. Samples covering 16 key respiratory viruses were included in this study. The concordance analysis above demonstrated a high positive percent agreement of over 97% between the TaqMan Assays for respiratory pathogens and other molecular platforms.

Scalable and flexible workflow

The QuantStudio family of instruments can meet any throughput requirement. For higher throughput laboratories, leverage our OpenArray nanofluidic technology. A complete end-to-end solution is available and includes the Thermo Scientific KingFisher Flex benchtop automated DNA/RNA extraction instrument, convenient extraction and purification kits, and the OpenArray AccuFill System. Combined, these systems can automate sample prep and liquid handling to enable the processing of hundreds to thousands of samples per day.    

To learn more about our testing solution, please watch the webinar: What You're Missing in Your Respiratory Pathogen Detection.

Whether you already own an instrument or are considering an expansion to your capabilities, your sample processing and throughput requirements can be addressed through different formats compatible with your instrument (i.e. TaqMan Array Cards, 96- or 384-well plates, or single tube assays) with made-to-stock or custom panels.


Ordering
 

Explore QuantStudio PCR Systems, a flexible and scalable real-time PCR platform.

Order the TaqMan™ Array Respiratory Tract Microbiota Comprehensive Card or TrueMark™ Respiratory Panel 2.0, TaqMan™ Array Card

Consulting-Services-Global_300x600

Services

Testing laboratories are under constant pressure to stay compliant with standards while expanding the number of molecular tests offered. When adopting a new testing method such as our real-time PCR RTI test solution, we can assist in validating the workflow to help ensure a successful launch.

Our analytical validation (AV) consulting services can speed up the launch process by up to 75% and cut costs by up to half. Our expert team will consult with you to develop and optimize your validation workflow, while providing data analysis support and template documentation to fully maximize your instrument and reagent investment.

Learn more ›

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