If you’re looking to culture human primary amniotic fluid cells or chorionic villus cells, AminoMAX™ media is the right product for you.  Our formulations are designed to increase cell attachment, growth rates and provide high metaphase yields.

Product Information

AmnioMAX™ C-100 medium was developed specifically for the in vitro diagnostic testing of human amniotic fluid specimens and chorionic villus sampling.  Designed to help minimize diagnostic turnaround time, AmnioMAX™ medium maximizes colony growth while maintaining the cytogenetic analyzability of resulting colonies.

AmnioMAX™-II Complete Medium is a second-generation formulation, further optimized for cell attachment and increased pH stability.  Supplied in a single bottle, it is complete with antibiotics and growth promoting agents, and requires no further supplementation.

AmnioMAX™ medium supports the growth of amniocytes (Figure 1) and delivers superior plating density.  These results demonstrate that human amniocytes cultured in AmnioMAX™ medium give rise to approximately 22% to 25% more colonies and 44% to 80% more cytogenetically analyzable colonies as compared to other amniocyte media.  In addition, as shown in Figure 2, the mean mitotic index rate of cultured primary amniocytes grown in AmnioMAX™ medium was significantly greater than that achieved with Chang medium.

Figure 1 - Growth kinetics of aminocytes cultured with AmnioMAX™ medium.  A representative growth curve of secondary aminocytes cultured in either AminoMAX™ medium or MEM-alpha supplemented with 20% FBS is shown.  Cell counts were taken at various intervals.  Results shown are the mean of duplicate samples.

Figure 2 - Mitotic rate comparison of AmnioMAX™ medium and Chang medium.  The relative mitotic index rate for primary aminocytes cultured in either AminoMAX™ medium or Chang medium in situ for 6 days was determined.  The number of mitotic events per 5,000 cells was scored for each culture medium.

leaf icon Sustainable solutions

We are committed to delivering products that serve the research needs of our customers, while striving to develop them in a way that minimizes our use of natural resources and our impact on the environment.

Learn more about sustainable solutions