The Class I SeCore® sequencing strategy begins with a multiplex PCR. Two fragments are amplified; one amplicon encompassing exon 2 and 3 and the other encompassing exon 4. The sequencing mixes are designed for bi-directional sequencing of each exon. The single amplification kits are available for A, B and C loci.
Group Specific Sequencing Primers (GSSPs) are available to resolve allele specific ambiguities.