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Evolved for choice and longevity—TaqMan SARS-CoV-2 2.0 assays

Choose from our two new advanced assays of Applied Biosystems TaqMan SARS-CoV-2 2.0 assays that deliver the same accuracy and reliability that our SARS-CoV-2 assays are known for, while offering the ability to accommodate multiple sample types and the choice to utilize either a standard or fast workflow. The standard workflow includes RNA extraction and traditional sample preparation, and the fast workflow is a fast, direct-to-PCR workflow with no RNA extraction required. You’ll also have confidence in your results knowing that our 2.0 assays include enhanced target redundancy to compensate for all SARS-CoV-2 mutations known now and those yet to be discovered in the future.  

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Features of the TaqMan SARS-CoV-2 2.0 assays include:

  • Same accuracy and reliability you have grown to trust—an advanced assay design using multiple targets on orf1a, orf1b, and N genes (Figure 1) compensates for current and emerging SARS-CoV-2 mutations, helping provide continued confidence in results now and into the future

  • Multiple workflow options—choose from two assays: one utilizes a standard workflow that includes RNA extraction (Figure 2), and the other utilizes a fast, direct-to-PCR workflow with no RNA extraction required (Figure 3)

  • Affordable and scalable—add to your existing SARS-CoV-2 menu using your current real-time PCR instrumentation
     


Product details for TaqMan 2.0 assays:

 

TaqMan SARS-CoV-2 Fast PCR Combo Kit 2.0

TaqMan SARS-CoV-2 RNase P Assay 2.0

 

Description

Fast, direct-to-PCR, raw saliva solution using an evolved assay design to enable widespread, high-frequency testing (Figure 3)

An evolved SARS-CoV-2 assay design, with RNase P for human sample confirmation (Figure 2)

 

Assay design

3 genomic regions (orf1a, orf1b, and N genes) with 8 total targets (Figure 1)

Internal control

RNase P

RNase P

 

Compatible sample types

Raw saliva collected with:

  • SpeciMAX raw saliva collection kit
  • Leak-proof, screw top tubes
  • Other conical tubes
  • RNA extracted from nasopharyngeal swabs and anterior nasal swabs

     

    Recommended sample preparation

    Lysis buffer (SalivaReady Solution, part of the TaqMan SARS-CoV-2 Fast PCR Combo Kit 2.0)

    KingFisher Flex Purification System with MagMAX Viral/Pathogen II Nucleic Acid Isolation Mix

    Recommended real-time PCR instruments

    Turnaround time (including sample prep)

    ~ 2 hours

    ~ 3 hours

    Recommended software

    QuantStudio Design & Analysis Software, RUO Edition 2.5 or higher


    Advanced 2.0 Assay Design

    COL114651-regions-target-image-new
    Figure 1. Advanced assay design for 2.0 assays.


    Recommended workflows

    COL114651-workflow1-image-new

    Figure 2. Recommended workflow for Taqman SARS-CoV-2 RNase P Assay 2.0.
     

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    Figure 3. Simplified, direct-to-PCR workflow for Taqman SARS-CoV-2 Fast PCR Combo Kit 2.0.
     

    Analysis Software