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Multiplex PCR NGS genotyping offers a simple, fast, and better workflow
The advanced chemistry and comprehensive coverage for AllType FASTplex is complemented with a streamlined workflow for minimal hands-on time and supported by intuitive software for easy sample auditing and enhanced data integration.
The AllType FASTplex assay offers a complete next generation sequencing solution that provides single tube amplification for all eleven HLA Class I and Class II loci, completely removing the need for amplicon pooling.
Features and benefits
Why choose the AllType FASTplex NGS solution?
- One multiplex PCR reaction per sample
- Early sample pooling
- Total hands-on time <90 minutes
- Automated data transfer and analysis
AllType FASTplex – quality with less complexity
The AllType FASTplex chemistry has been enhanced to improve performance, robustness, and reliability. With the addition of Class II exon 1 primer mix, the AllType FASTplex reagents reduce ambiguities, provide comprehensive coverage across 11 loci, and generate highly concordant results at 99.8% or higher, all in a single PCR reaction.
Next-Generation Sequencing with the AllType FASTplex NGS Assay
NGS is a powerful but complex technology genotyping. An intelligently simple approach, the One Lambda AllType FASTplex NGS Assay is the only easy, single-test solution supported by streamlined and integrated software.
Coverage
Figure 1. AllType FASTplex exon coverage.
Workflow
Workflow simplicity and reduced hands-on time
The assay can enhance your lab’s efficiency with reduced labor, pipetting, and risk of contamination. By combining fragmentation and barcoding steps into a single reaction, the workflow allows for samples to be pooled early in the protocol so that library preparation can be completed in a single tube.
The protocol has been designed for simplicity, easy implementation, and greater cross-training in the lab. From start to finish, the workflow can be performed in under 7 hours with less than 90 minutes of hands-on time.
Figure 2. AllType FASTplex workflow.
Analysis
When the workflow is complete, our TypeStream Visual NGS Analysis Software will begin the automatic analysis of your data while you’re away from your desk.
By eliminating manual data transcription, the software simplifies your data reporting and eliminates opportunities for errors.
Results can also be combined in our HLA Fusion Software for advanced analytics.
Protocol comparison
Choose the right NGS assay, protocol, and coverage for your lab
Our AllType and AllType FASTplex NGS assays apply a single multiplex reaction to amplify all major Class I and Class II loci, completely removing the need for amplicon pooling. Both provide scalable solutions for low- and high-volume laboratories.
| AllType FASTplex NGS | AllType NGS | ||
| Method | Multiplex long-range amplification | Multiplex long-range amplification | |
| Turnaround time | 1-2 days
|
2-3 days | |
| Complexity | Medium (does not require specialized training of lab personnel) |
High | |
| Resolution | Ultra-high | Ultra-high | |
| Throughput | High | High | |
| Regulatory status | RUO Refer to products for details |
RUO and IVD Refer to products for details |
|
| Instruments | Illumina MiSeq, MiniSeq, iSeq 100, Ion GeneStudio S5, and Ion GeneStudio S5 plus | Illumina MiSeq, MiniSeq, iSeq 100, Ion GeneStudio S5, and Ion GeneStudio S5 plus | |
* Considers one technologist preparing 24 samples.
Resources
Not all products are CE marked or have 510(k) clearance for sale in the US. Availability of product in each country depends on local regulatory marketing authorization status. Please consult your local sales representative for details. MiSeq, MiniSeq, and iSeq are trademarks of Illumina, Inc.