Electron Microscopy

Drug Discovery Using Electron Microscopy

Cryo electron microscopy tools for cutting-edge pharma drug design.

As targets become more challenging, solving structures of your drug target molecules or biologics also becomes increasingly difficult. Structure-based drug design is not always a given, but cryo-electron microscopy (cryo-EM), a powerful biophysical technique that can analyze molecular assemblies, is changing the paradigm. By adding cryo-EM to your portfolio of methods, all your projects can now be structure-enabled.

Whether you are a structural biologist or a medicinal chemist, cryo-EM allows you to visualize anything, from small to large molecules, at near-atomic resolution. With this level of detail, you can obtain time-critical insight for your particular drug discovery project.

Learn how cryo-EM empowers you to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.

 

Cryo-EM can help accelerate your drug design

Cryo-EM, comprised of a variety of techniques, including single-particle analysis (SPA) and micro-electron diffraction (microED), is designed to determine the structures of proteins and small molecules. Using cryo-EM can help you increase the speed of your rational drug discovery and overcome your toughest structural chemistry challenges.

Cryo-EM can enable faster identification of best-in-class drugs by visualizing drug-target complexes, giving you key insight into the interactions of anything from small molecules to large macromolecular assemblies. By using single particle analysis, you can determine near-atomic level molecular structures, helping every compound reach its full potential, from hit to lead. Cryo-EM excels at exposing the structure of biologics as well, providing critical information for everything from therapeutic antibody epitope mapping to increasingly large biopharmaceuticals.

 

Single-particle analysis: no need for crystals

Single-particle analysis vitrification (rapid plunge freezing) allows you to sidestep crystallization while facilitating protein purification. Because single particle analysis does not require sample crystallization or even recombinant overproduction, it is an incredibly versatile method that is well-suited for a wide range of targets, particularly membrane proteins and large assemblies in their native state. Our single-particle analysis workflow combines cutting-edge hardware with dedicated automated sample screening software, allowing you to optimize your biochemistry and vitrification parameters before transferring your best samples to the cryo-transmission electron microscope (cryo-TEM) for the ultimate 3D structure analysis.

From protein sequence to structure

One of the greatest advantages of cryo-EM relative to conventional structural biology techniques is its ability to analyze large, complex and flexible structures. Often, these cannot be crystallized for X-ray crystallography (XRD) or are too large and complex for nuclear magnetic resonance (NMR) spectroscopy. These include many biologically important proteins, especially those with variable or flexible structures, such as membrane proteins. Our dedicated SPA workflow has been developed around our instruments to ensure a smooth pathway from sample preparation to image acquisition to data processing. This purpose-driven design can help a wider range of structural biology labs achieve faster time-to-data when determining the 3D structure of proteins and protein complexes at near-atomic resolution.

Explore the simplicity of our single particle analysis workflow ›

MicroED: structural chemistry with electrons

MicroED data is acquired using electrons instead of X-rays. Because of the stronger interaction of electrons with much smaller crystals, you can obtain a solvable structure from mere nanocrystals of your compound. Studies have also shown the potential for impressive time-to-structure results of just a few hours. This opens unheard of avenues of structural characterization for natural products, precious ligands and metabolites. Not only that, but microED also makes polymorph identification possible.

Learn how microED helps accelerate time-to-structure results ›

 

 

Cryo-EM within your reach

Getting started with cryo-EM

You have a partner! With our help, setting up a cryo-electron microscopy facility can be easier than you imagined. We will be with you every step of the way, from discussing flexible financing options, to building planning, to initial sample testing and installation. Our goal is to get you up and running as quickly and efficiently as possible. You can also take advantage of fee-for-service providers, cryo-EM training, and more.

Review our tailor-made cryo-EM SPA solution ›

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Now is the time to adopt cryo-EM

It’s time to invest in cryo-EM today. Early cryo-EM adopters in the pharma industry already see its benefits. Find out why pharmaceutical leaders around the globe have invested in cryo-EM for drug discovery and learn how their motivations may align with your goals.

Read testimonials ›

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Setting up for ongoing success

From day one to year one and beyond, you can rely on our expertise to support your investment and research. Our highly knowledgeable cryo-EM application specialists can assist with everything from sample preparation to data processing. Our Cryo-EM University combines online instruction with practical training to transform your lab personnel into a qualified team of cryo-electron microscopists. You can rely on us, a cryo-EM leader currently supporting more than 100 systems worldwide, for long term ongoing support.

Learn about our cryo-EM support expertise ›

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Planning and building advice

We are experts in creating cryo-EM labs. Supporting more than 100 high-end cryo-EM facilities around the world, we offer unparalleled knowledge and expertise. We will perform a site survey to make sure your site meets all room readiness requirements (including vibrations, magnetic fields, temperature stability, and humidity control) prior to installation. We will provide advice on IT infrastructure and resources required to handle big data, environmental conditions needed for the facility, and we will help you to determine what auxiliary lab equipment you might need. Our expertise and support are designed to get you up and running quickly.

Learn more from our in-depth planning guide ›

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Financial resources

We provide a variety of ways to meet your financial needs, including assistance with leasing and financing options. Avoid unexpected expenses by letting us help you properly plan for building and/or remodeling, determine IT infrastructure expenditures and operational costs, including personnel needs, power consumption, and data storage.

Review available financing options ›

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Increase your cryo-EM knowledge

Your lab personnel can quickly turn into cryo-EM experts by watching our online Cryo-EM University videos and with practical training from our dedicated application specialists. This exciting free course, created in collaboration with Professor Grant Jensen of Caltech University, combines online instruction with practical training to transform your personnel into cryo-electron microscopists. Learn a range of cryo-EM essentials, including step-by-step single particle analysis (SPA) instructions, expert demonstrations, and theoretical lessons, all available at your pace, wherever you want.

Enroll in Cryo-EM University ›

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Cryo-EM as a service

If you want to take advantage of cryo-EM’s exciting applications in biology but find the cost of in-house adoption too prohibitive, there are options for you. We partner with a number of cryo-EM service providers around the world:

Cryo-EM? We have an app for that.

With an increasing number of new structural biologists making the transition into cryo-TEM, we focus on enhancing optimal tool performance, simplification of the user interface, and automated guidance of experimental settings. We are pushing the high-resolution performance and throughput of our instruments by combining hardware improvements with advanced software capabilities.

Our instruments’ tailor-made user interface makes operation an intuitive process, especially when combined with our easy-to-use app. This Scientific Workflows app guides you through the cryo-EM single-particle analysis workflow and offers advanced calculations like recommended data acquisition magnification and estimated acquisition time. It also contains step-by-step guides, similar to standard operating procedures, tailored to a given set of system configurations and sample/grid characteristics. If more assistance is required, we also have applications experts available for on-site support and training, ensuring you are ready to perform your research with a high level of confidence in your results.

Download the free app:

 

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MicroED

MicroED is an exciting new technique with applications in the structural determination of small molecules and protein. With this method, atomic details can be extracted from individual nanocrystals (<200 nm in size), even in a heterogeneous mixture.

Learn more ›

Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

MicroED

MicroED is an exciting new technique with applications in the structural determination of small molecules and protein. With this method, atomic details can be extracted from individual nanocrystals (<200 nm in size), even in a heterogeneous mixture.

Learn more ›

Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Samples


Proteins Analysis

Cryo electron microscopy provides near-atomic resolution 3D protein structure. It can determine structural information for complexes and crystallization-resistant samples, as well as vital cellular context.

Learn more ›


Biopharmaceutical Research

Structural drug discovery is enabled by cryo electron microscopy, as the method provides near-atomic-resolution detail for small molecules and protein biologics in their fully hydrated state.

Learn more ›


Virus Analysis

Cryo-EM enables the 3D structural visualization of virus particles, and the antigen-antibody interface, at near-atomic resolutions. A virus’s inherent structural symmetry makes it the ideal target for cryo EM analysis.

Learn more ›

Products

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Krios G4 Cryo-TEM for
Life Sciences

  • Improved ergonomics
  • Fits more easily into new and existing labs
  • Maximized productivity and automation
  • Best image quality for high-resolution 3D reconstruction

Glacios Cryo-TEM for
Life Sciences

  • Cryo-TEM
  • Small Footprint
  • Flexible Accelerating Voltage 80-200 kV
  • High Brightness X-FEG Electron Gun

Aquilos 2 Cryo-FIB for Life Sciences

  • Automation enables production of multiple lamellas
  • Target and extract your structure of interest with lift-out nano-manipulator
  • 3D visualization for high-resolution tomography

Talos Arctica TEM for
Life Sciences

  • Increased data acquisition speed
  • High data with robotic sample handling & automated loading
  • Unattended platform operation and automated data acquisition
  • Low cost of ownership with remote diagnostics and preventive service

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Electron microscopy services for
the life sciences

To ensure optimal system performance, we provide you access to a world-class network of field service experts, technical support, and certified spare parts.

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