Accessories for Electron Microscopes

Falcon 4 Direct Electron Detector

Direct electron detection with enhanced image quality and throughput.


The Thermo Scientific Falcon 4 Direct Electron Detector is the ideal solution for your single particle analysis and cryo-electron tomography needs. By combining a 10x speed improvement (as compared to the Falcon 3 Detector) with an enhanced detector quantum efficiency (DQE) over the entire spatial frequency range, the Falcon 4 Detector allows you to collect the best quality images with the highest throughput possible. In fact, the unsurpassed DQE at low frequencies makes it ideally suited for small or difficult to detect proteins.

Additionally, the Falcon 4 Detector comes with EPU Data Management (powered by Athena Software) which offers easy project administration and automated organization of images and metadata for remote and collaborative access.

Note: The Falcon 4 Direct Electron Detector is fully compatible with the Thermo Scientific Krios G4 Cryo-TEM and can also be retrofitted on existing Krios, Glacios, Talos Arctica and other 200 kV Talos TEMs.

Key Features

  • Leading detective quantum efficiency (DQE) across spatial frequency range
  • 10x speed improvement over its predecessor
  • Fully embedded into Thermo Scientific EPU and Tomography software
  • Built-in data management for remote and collaborative access

Specifications

Style Sheet for Products Table Specifications
Camera architecture Direct electron detection
Sensor size 4,096 × 4,096 pixels, ~ 5.7 x 5.7 cm
Pixel size 14 x 14 μm2
Imaging performance 4k x 4k DQE
Operating voltage 200 kV, 300 kV
Mounting position On-axis, bottom mounted, retractable
Frame rate 250 fps
Lifetime 5 years in normal use (1.5 Gpe yields <10% DQE degradation)
Detection modes
  • EC: electron counting
  • Linear: integration mode
Style Sheet for Media Tabs
GABA receptor imaged with the Falcon Direct Electron Detector.
3D reconstructions of the GABA receptor membrane protein in a nanodisk, bound to the drug Ro-15-4513. Displayed resolution is 2.75 Å. Data collected on a Krios Cryo-TEM with the Falcon 4 Detector. Images Courtesy of Simonas Masiulis, Radu Aricescu, MRC-LMB Cambridge and Evgenia Pechnikova, Abhay Kotecha, Thermo Fisher Scientific.
20S Proteasome imaged with the Falcon 4 Direct Electron Detector.
3D reconstruction of the 20S proteasome from Thermoplasma acidophilum at 2.0 Å resolution. Data collected on the Krios G4 Cryo-TEM with the Falcon 4 Detector. Image courtesy of Dr. Juergen Plitzko and Dr. Philipp Erdmann, Max Planck Institute for Biochemistry, Martinsried, Germany.
GABA receptor imaged with the Falcon Direct Electron Detector.
3D reconstructions of the GABA receptor membrane protein in a nanodisk, bound to the drug Ro-15-4513. Displayed resolution is 2.75 Å. Data collected on a Krios Cryo-TEM with the Falcon 4 Detector. Images Courtesy of Simonas Masiulis, Radu Aricescu, MRC-LMB Cambridge and Evgenia Pechnikova, Abhay Kotecha, Thermo Fisher Scientific.
20S Proteasome imaged with the Falcon 4 Direct Electron Detector.
3D reconstruction of the 20S proteasome from Thermoplasma acidophilum at 2.0 Å resolution. Data collected on the Krios G4 Cryo-TEM with the Falcon 4 Detector. Image courtesy of Dr. Juergen Plitzko and Dr. Philipp Erdmann, Max Planck Institute for Biochemistry, Martinsried, Germany.

Applications

Infectious_Disease_Research_App_w274x180

Infectious Disease Research

Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.

DrugDiscovery_Appl_274x180

Drug Discovery

Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.

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Plant Biology Research

Fundamental plant biology research is enabled by cryo electron microscopy, which provides information on proteins (with single particle analysis), to their cellular context (with tomography), all the way up to the overall structure of the plant (large volume analysis).

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Pathology Research

Transmission electron microscopy (TEM) is used when the nature of the disease cannot be established via alternative methods. With nano-biological imaging, TEM provides accurate and reliable insight for certain pathologies.

Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Integrative Structural Biology

To understand protein function, you need complex and structure information beyond individual proteins. Integrative structural biology combines mass spectrometry and cryo EM for the determination of large dynamic complex structure.

Learn more ›

Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Integrative Structural Biology

To understand protein function, you need complex and structure information beyond individual proteins. Integrative structural biology combines mass spectrometry and cryo EM for the determination of large dynamic complex structure.

Learn more ›

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