Available on Thermo Scientific Krios and Glacios Cryo-TEMs, the Thermo Scientific Selectris and Selectris X Imaging Filters are post-column imaging filters that improve the contrast of TEM images, resulting in high-resolution structures up to atomic resolution. The zero-loss filtering of the Selectris Filters removes noise caused by inelastically scattered electrons, producing an increased signal-to-noise ratio (SNR) and better contrast. Designed for high stability, the zero-loss peak position of the Selectris Filters is insensitive to temperature variations in the environment, eliminating the need for frequent tuning.

With the Selectris Filters, zero-loss energy filtering is straightforward due to the thorough integration of software and hardware along with extensive automation and exceptional stability. Every mechanical and electron-optical element has been designed for stability and data reproducibility, enabling the unattended and reliable acquisition of large datasets with narrow energy slit widths (<10 eV). The capability to use these narrow slit widths provides an additional boost in contrast for single particle cryo-electron microscopy (cryo-EM), enhancing signal strength, resolution, and throughput.

Paired with the latest generation Thermo Scientific Falcon 4 Direct Electron Detector, Selectris Filters allow you to obtain high-resolution structures quickly, revealing new biological insights. To further increase the productivity and ease-of-use of your cryo-EM workflows for single particle analysis and cryo-electron tomography, these filters are fully integrated into Thermo Scientific EPU Software, Thermo Scientific Tomography Software, and our instrument operation software.

On the rare occasion that filter tuning is necessary, it can be completed automatically within minutes; this ensures smooth daily operation and efficient data acquisition.

Cryo-electron microscopy atomic-resolution of apoferritin.
Atomic-resolution structure of apoferritin, resolved by cryo-electron microscopy.

Selectris X Filter – taking the next step toward atomic resolution

Expanding on the stability, ease-of-use, and performance of the Selectris platform, the Selectris X Imaging Filter offers an even more sophisticated electron optical system for further aberration correction. This results in extremely low distortion characteristics in both the image and energy domains, opening the way to true atomic-resolution structures.


Key Features

Designed for stability

  • Narrow (<10 eV) zero-loss energy filtering offers enhanced contrast for higher resolution reconstructions
  • Sophisticated aberration correction, low image distortions, and uniform energy resolution over entire field of view
  • Minimized sensitivity to temperature variations

Straightforward operation

  • Fully integrated with Thermo Scientific instrument operation software, as well as EPU Software and Tomography Software for data collection.
  • Automated filter tuning completed in minutes
  • No need to interrupt data collection for zero-loss centering

Falcon 4 Direct Electron Detector

  • High detective quantum efficiency (DQE)
  • High speed, routinely capable of producing 300+ movies/hour
  • Built-in electron event representation (EER) for full temporal resolution (240 fps to disk) - no need for fractionating at time of acquisition
  • Super resolution without file size penalty

Specifications

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Imaging Filter

Position Bottom mounted
Stability Slit drift < 1eV/K
Zero-loss centering Automated
Filter tuning Automated
TEM operating voltage 200 kV, 300 kV


Direct Electron Detector Camera

Sensor 4,096 × 4,096 pixels, ~ 5.7 x 5.7 cm
Pixel size 14 x 14 um2 pixel size
File formats
  • EER super-resolution up to 16k x 16k.  All counted frames (~240 fps) accessible/written to disk, fractionation not required
  • MRC
Detection modes
  • Electron counting mode
  • Survey mode (fast linear mode)
Automation
  • Integrated in Thermo Scientific EPU and Tomography software
  • Compatible with 3rd party software via TEMscripting
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Cryo-EM: What's Next

Register to view on-demand presentations covering the Selectris capabilities, a behind-the-scenes look at R&D filter development, and record-breaking structure resolution results for single particle analysis and cellular cryo-tomography using the Selectris Filter. Learn about the potential applications with high resolution for small proteins (<100kDa), proper model building and corrected chemistries.

Register now

Cryo-EM: What's Next

Register to view on-demand presentations covering the Selectris capabilities, a behind-the-scenes look at R&D filter development, and record-breaking structure resolution results for single particle analysis and cellular cryo-tomography using the Selectris Filter. Learn about the potential applications with high resolution for small proteins (<100kDa), proper model building and corrected chemistries.

Register now

Applications

Life_Sciences_Application_274x180_144DPI

Structural Biology Research
 

Cryo-electron microscopy enables the structural analysis of challenging biological targets such as large complexes, flexible species and membrane protein.

DrugDiscovery_Appl_274x180

Drug Discovery
 

Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.

Infectious_Disease_Research_App_w274x180

Infectious Disease Research
 

Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.

Single Particle Analysis

Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Integrative Structural Biology

To understand protein function, you need complex and structure information beyond individual proteins. Integrative structural biology combines mass spectrometry and cryo EM for the determination of large dynamic complex structure.

Learn more ›

Single Particle Analysis

Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Integrative Structural Biology

To understand protein function, you need complex and structure information beyond individual proteins. Integrative structural biology combines mass spectrometry and cryo EM for the determination of large dynamic complex structure.

Learn more ›

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Related Products

Krios G4 Cryo-TEM
Glacios Cryo-TEM
Falcon 4
EPU 2 Software
Tomography 4 Software
EM Services for Cryo-Electron Tomography
EM Services for SPA


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