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The Thermo Scientific Aquilos 2 Cryo-FIB is the latest generation of our cryo-DualBeam system. It is dedicated to the preparation of thin, electron-transparent lamellas for high-resolution cryo-electron tomography or MicroED of micro-crystals. 

With the Aquilos 2 Cryo-FIB, you can take a revolutionary step forward by automating steps in the workflow to produce multiple lamellas from specific programmed locations. The improved dedicated cryo-hardware will provide you with longer run times at ultra-low contamination rates. Discover how our latest cryo-FIB platform enables the determination of structures right inside the cell.

The tomography of biomolecules in their cellular context requires highly accurate sample preparation with cryo-FIB, which can be achieved with information from integrated fluorescence microscopy. Now available for the Aquilos 2 Cryo-FIB, the Thermo Scientific iFLM Correlative System combines light and electron microscopy in one system. This eliminates the need for extra sample transfer steps and enables you to create a more streamlined cryo-correlative solution for cryo-tomography. The iFLM (Integrated Fluorescence Light Microscope) Correlative System localizes fluorescent targets inside the chamber, allowing you to correlate two imaging modalities within one system, thereby ensuring the sample is in the right location.

Cryo-lamella prepared with the Aquilos 2 Cryo-FIB
Cryo-lamella prepared with the Aquilos 2 Cryo-FIB (left). Overlay of SEM image with fluorescence image obtained with the iFLM Correlative System (right).
Aquilos 2 Cryo-FIB with the iFLM Correlative System.
Aquilos 2 Cryo-FIB with the iFLM Correlative System.

Benefits

Automation

Automation

Run the system overnight, allowing it to automatically produce multiple lamellas for you with Thermo Scientific AutoTEM Software.

Cryo-Lift-out

Cryo-Lift-out

Target and extract your structure of interest with the Thermo Scientific EasyLift NanoManipulator, our high-precision cryo-lift-out solution.

3D visualization

3D visualization

View the native subcellular structure below the surface using charging contrast and identify features for high-resolution tomography in cryo-TEM with Thermo Scientific Auto Slice and View Software.

Extended run time

Extended run time

Your samples remain at vitreous temperatures, even for overnight runs. Cryo-hardware and a fully rotatable cryo-stage protect the frozen samples from contamination and ensure they stay vitrified for the duration of your experiments.

Thermo Scientific Aquilos 2 Cryo-FIB cryo sample prep tool.
DualBeam FIB-SEM Microscopes

Aquilos 2 Cryo FIB

Cryo-EM sample preparation tool with extended run time and enhanced automation.



Key Features

Automated batch cryo-lamella milling

Mark multiple points of interest, such as cells, for cryo-lamella milling. Cryo AutoTEM Software is a guided solution for automatically preparing multiple lamellas in unattended runs, increasing your productivity.

Cryo-lift-out lamella preparation

Prepare lamellas from specific targeted regions with nanometer-position accuracy. The high-precision EasyLift NanoManipulator option allows for extraction of site-specific regions, such as fluorescently labeled proteins, even from high-pressure frozen samples. These lamellas can subsequently be placed inside autogrids for cryo-TEM and further imaging of protein structures and protein networks.

3D visualization

With our Cryo-Auto Slice and View Software, you can acquire 3D images from vitrified samples by sequential milling and then imaging of the cross-section of a defined volume of the sample. This process will help you stop milling at the correct subcellular region to prepare your lamella before you observe the ultrastructural detail in your cryo-TEM using cryo-ET.

Correlate light and electron within one system

The iFLM Correlative System allows samples to be imaged directly within the high vacuum of the Aquilos 2 Cryo-FIB without additional transfer steps from an external cryo-light microscope. This saves time and reduces the risk of sample contamination. Fluorescently marked cells can be located directly in the Aquilos.

Accelerate and Advance service packages for Cryo-tomography

Accelerate and Advance service packages for Cryo-tomography will support your success from the moment your purchase your Thermo Scientific Aquilos 2 Cryo-FIB system.  From applications support to system remote monitoring to comprehensive maintenance, our unique blend of data-driven and hand-on services will help you quickly achieve your desired outcomes.

Preparation of cells for cryo-electron tomography

The Thermo Fisher cryo-electron tomography workflow covers flash freezing cells to final 3D visualization. Interior cellular regions are imaged at nanometer-scale resolution and from cryo-lamellae precisely prepared with Aquilos 2 cryo-FIB.


Specifications

Style Sheet for Products Table Specifications
Electron Optics
 
Accelerating voltage range:200 V – 30 kV
Beam current range:1.5 pA to 400 nA
Resolution (with cryo-stage):1.6 nm at 30 kV, 2.6 nm at 2 kV (at room temperature), 6.0 nm* at 2kV (at cryo-temperature)
Resolution (with cryo-stage):7.0 nm at 30 kV
Ion Optics
 
Source lifetime: 1300 hours
Voltage:500 V to 30 kV
Beam current:1.5 pA – 65 nA in 15 steps
Resolution (with cryo-stage):7.0 nm at 30 kV
Cryo-stage
 
Tilt range at cryo (eucentric WD):-15° to +55°
XY range:110 mm
Z range:65 mm
Rotation:360° (endless)
Cooldown time: <20 minutes
Vacuum system
 
Chamber vacuum at room temperature: <4e–4Pa
Chamber vacuum at cryo-conditions: <8e–5Pa

Resources


Applications

Infectious_Disease_Research_App_w274x180

Infectious Disease Research

Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.

Life_Sciences_Application_274x180_144DPI

Structural Biology Research

Cryo-electron microscopy enables the structural analysis of challenging biological targets such as large complexes, flexible species and membrane protein.

DrugDiscovery_Appl_274x180

Drug Discovery

Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.


Techniques

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›


Contact us

Electron microscopy services for
the life sciences

To ensure optimal system performance, we provide you access to a world-class network of field service experts, technical support, and certified spare parts.

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