Sample Preparation Equipment for Electron Microscopy

Cryo EM Sample Preparation with the Vitrobot System

Reproducible vitrification of biological samples.


Vitrification forms an amorphous solid that does little to no damage to the sample structure and is a critical technique for cellular and structural biology research where samples are cooled so rapidly that the surrounding water molecules do not have time to crystallize.

Good vitrification is a crucial step within structural biology applications such as single particle analysis (SPA)cryo electron tomography (cryo-ET), and MicroED. The Thermo Scientific Vitrobot Mark IV System ensures that critical vitrification parameters can be kept constant, thereby ensuring enhanced sample quality prior to imaging of the vitrified particles or cellular components.

The Vitrobot System is a specimen preparation unit that offers great value to the demanding scientific areas of cell biology and molecular imaging. It is equally suitable for food, industrial, pharmaceutical, and nanotechnological applications - where the true colloidal structure of your analyte is critical.

Cryo TEM image of apoferritin protein complexes vitrified with the Vitrobot System.
Cryo-TEM image of apoferritin protein complexes vitrified on the Vitrobot System.

Key Features

  • Fully automated, reproducible vitrification of aqueous suspensions
  • Precise control of critical process parameters
  • Enclosed environmental chamber
  • High sample throughput
  • Easy and flexible user interface
  • Semi-automated grid transfer

Specifications

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Weight 31 kg
Dimensions (l/w/h) 413 mm x 20 mm x 890 mm
Power Supply 110 - 230 V
50 - 60 Hz
Fuse: 4 AT (110 V USA)
Fuse: 2 AT (230 V Europe)
Power cable 90 - 250 V

 

Operating Parameters
 
Working Temperature 4 – 60 °C (at an ambient temperature range between 18 – 25 °C)
  Peltier controlled heating/cooling
  Maintain relative humidity at 100%
  Ultrasonic controlled humidification
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Applications

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Drug Discovery
 

Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.

Infectious_Disease_Research_App_w274x180

Infectious Disease Research
 

Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.

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Structural Biology Research
 

Cryo-electron microscopy enables the structural analysis of challenging biological targets such as large complexes, flexible species and membrane protein.

Single Particle Analysis

Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

MicroED

MicroED is an exciting new technique with applications in the structural determination of small molecules and protein. With this method, atomic details can be extracted from individual nanocrystals (<200 nm in size), even in a heterogeneous mixture.

Learn more ›

Single Particle Analysis

Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Cryo-Tomography

Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

MicroED

MicroED is an exciting new technique with applications in the structural determination of small molecules and protein. With this method, atomic details can be extracted from individual nanocrystals (<200 nm in size), even in a heterogeneous mixture.

Learn more ›

Samples


Proteins Analysis

Cryo-electron microscopy provides near-atomic resolution 3D protein structure. It can determine structural information for complexes and crystallization-resistant samples, as well as vital cellular context.

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Biopharmaceutical Research

Structural drug discovery is enabled by cryo-electron microscopy, as the method provides near-atomic-resolution detail for small molecules and protein biologics in their fully hydrated state.

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Virus Analysis

Cryo-EM enables the 3D structural visualization of virus particles, and the antigen-antibody interface, at near-atomic resolutions. A virus’s inherent structural symmetry makes it the ideal target for cryo-EM analysis.

Learn more ›

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