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Glacios 2 Cryo-TEM overview

The Thermo Scientific Glacios 2 Cryo-Transmission Electron Microscope (cryo-TEM) allows you to easily collect near atomic data from a broad range of biological targets. Compared to the previous generation, the Glacios 2 Cryo-TEM delivers higher throughput and makes cryo-EM more accessible. It features an integrated Thermo Scientific Falcon 4i Direct Electron Detector, Thermo Scientific EPU Software, and a new full enclosure, which combine to enhance image quality, automate data acquisition, and simplify your work. The Glacios 2 Cryo-TEM is ideally suited for single-particle analysiscryo-electron tomography (cryo-ET), and micro-electron diffraction (MicroED).

Meet the Glacios 2 Cryo Transmission Electron Microscope

Glacios 2 Cryo-TEM benefits

Better results

Better results

The combination of the Falcon 4i Direct Electron Detector, the newly designed enclosure, and Smart EPU Software work together to guide the microscope and user to get the best possible results from their samples.


Less hands-on time

Smart EPU Software’s automation and AI-driven plugins guide you through critical decision points, and in some cases make decisions automatically, reducing the need for manual intervention


Faster data collection

The Falcon 4i Direct Electron Detector can take up to 320 fps while fringe-free imaging (FFI) and automation combined with Smart Plugins help your experiment move quickly from one step to the next.


Simplified workflow

Smart EPU software assists with experimental set up and provides real-time feedback throughout the experiment, reducing the need for prior expertise.



Learn more about the Glacios 2 Cryo-TEM in our 30-minute webinar. We’ll cover:

  • How to make impactful structural biology discoveries
  • The higher-throughput and ease-of-use of Glacios 2 Cryo TEM
  • And more ...

Register to watch

Key features of the Glacios 2 Cryo-TEM

High-resolution TEM

The Glacios 2 Cryo-TEM provides near atomic resolution. With improved hardware and a new enclosure that minimizes environmental influences, the Glacios 2 Cryo-TEM now has an information limit of 2.1 Å. Similarly, transmission loss due to ice growth has been reduced to 2% over 24 hours, helping you collect more high-resolution images with less effort. When the Glacios 2 Cryo-TEM is configured with a Thermo Scientific Selectris X Imaging Filter, the speed and resolution can be further improved.

Protein structure of Apoferritin reconstructed from data collected on a Glacios Cryo-TEM
  • Apoferritin (450 kDa)
  • Acquisition time: ~7 hours
  • 1.6 Å resolution
Protein structure of CDK cyclin reconstructed from data collected on a Glacios Cryo-TEM
  • CDK-cyclin (85 kDa)
  • Acquisition time: ~22 hours
  • 2.3 Å resolution
Protein structure of GABA alpha receptor reconstructed from data collected on a Glacios Cryo-TEM
  • GABAA Receptor (210 kDa)
  • Acquisition time: ~12 hours
  • 2.4 Å resolution
Protein structure of GLP1 receptor reconstructed from data collected on a Glacios Cryo-TEM
  • GLP1 Receptor (150 kDa)
  • Acquisition time: ~20 hours
  • 2.6 Å resolution

High throughput Cryo-TEM image acquisition

The integrated Falcon 4i Direct Electron Detector provides better image quality than its predecessors in less time. To further enhance productivity and image quality, the Glacios 2 Cryo-TEM offers fringe-free imaging (FFI) that eliminates fringes at the edge of the beam, a more stable stage, and faster automated data acquisition. Together, these improvements allow you to collect more images per foil hole and accelerate single particle analysis per foil hole and accelerate single particle analysis and cryo-ET. Adding the Selectris X Imaging Filter allows you to use a very stable narrow slit width (<10 eV) for an additional boost in contrast for single particle cryo-EM and cryo-ET, improving resolution and obtaining results faster with less data to maximize resolution.

See how the Falcon 4i Direct Electron Detector enhances throughput with 10 times shorter exposure times than previous generations.

Aberration-free image shift (AFIS) is an optical mode for faster data acquisition without comprising image quality. AFIS performs large beam shifts without off-axis coma and astigmatism.

Acquire more images per sample area with FFI, enhancing your daily data throughput. Hear how FFI has been helping the daily research in Dr. Elizabeth Villa’s lab.

Maximized ease of use and efficiency

Smart EPU Software enables faster and easier set up and processing, benefiting both expert and non-expert microscopists. Several critical set-up steps have been automated, such as alignment of the column and repair of system status, when possible. In addition, the software and various optional add-ons can help users get the most out of their microscope time and the best possible results.

  • Optional Smart EPU Software with EPU Quality Monitor is an AI-enabled software solution capable of analyzing intermediate results, providing instant feedback and steering data collection on the fly.
  • Optional Smart EPU Software with Embedded CryoSPARC Live is a turnkey solution for real-time structural feedback on data quality and comes with full service and application support.
  • Optional EPU Multigrid Software allows data collection for up to 12 grids to be completed while away from the microscope, ensuring maximum productivity.
  • Open application programming interface (API) enables the development of your own functionality for the Glacios 2 Cryo-TEM.

Workflow connectivity

Sample screening is an essential component of successful cryo-EM workflows, allowing verification of sample quality in terms of both biochemistry and vitrification. The Glacios 2 Cryo-TEM fits seamlessly into single particle analysis and cryo-ET workflows with convenient and contamination-free sample transfer throughout the workflow.

The Glacios 2 Cryo-TEM easily connects to workflows on other Thermo Scientific instruments, including:

  • Thermo Scientific Krios Cryo-TEM
  • Thermo Scientific Tundra Cryo-TEM
  • Thermo Scientific Talos Arctica Cryo-TEM
  • Thermo Scientific Aquilos 2 Cryo-FIB
  • Thermo Scientific Arctis Cryo Plasma FIB

In addition to optimal mechanical connectivity, Smart EPU data management, powered by Thermo Scientific Athena Software, enables data connectivity for reproducible operation and storage of acquired data.

Smaller footprint

The hardware architecture of the Glacios 2 Cryo-TEM has been specifically designed with a smaller footprint and easier access path compared to other 200kV microscopes with automated sample loading systems.

Glacios 2 Cryo-TEM specifications

  • High-brightness field emission gun (X-FEG)
Accelerating voltage
  • Flexible accelerating voltage from 80–200 kV
  • Cryo-Autoloader for automated and contamination-free loading of cassettes containing up to 12 Autogrids
  • Automatic condenser, objective, and SA apertures
  • Symmetric constant power objective lens (minimize image aberrations and lens hysteresis during mode switching)
  • Symmetric constant power C-TWIN objective lens with wide-gap pole piece (11 mm)
  • Computerized 4-axis specimen stage with ±70° alpha tilt
  • Cryo-stage with single-axis holder
  • Rotation-free imaging upon magnification changes
Installation requirement highlights
  • Environmental temperature: 18°C–23°C
  • Door height: 2.32 m (optionally reduced to 2.00 m)
  • Door width: 0.99 m
  • Ceiling height: 2.80 m
AFIS (aberration-free image shift)
  • Enhancing throughput with shorter relaxation times when moving coma-free between grid holes
FFI (fringe-free imaging)
  • Fringe-free imaging (FFI) for multiple image acquisitions per grid hole
    Smart EPU Software
    • Thermo Scientific EPU 3 Software for automated sample screening and data acquisition (includes open application programming interface)
    • EPU Multigrid functionality (optional)
    • EPU Quality Monitor Software (optional)
    • Embedded CryoSPARC Live (optional)
    Additional components
    • Primary control unit with two 24” monitors to be placed within 10 m of the  column
      Detectors (optional)
      • Falcon 4i Direct Electron Detector
      • Thermo Scientific Ceta-D Camera
      • Thermo Scientific Ceta-M Camera
      • HAADF STEM detectors*
      • On-axis BF/DF detectors*
      • *STEM is not compatible with FFI
      Energy filter (optional)
      • Selectris and Selectris X Imaging Filters

        Glacios 2 Cryo-TEM software

        For the different techniques performed on the Glacios 2 Cryo-TEM, we have developed software solutions to help you get the most out of your instrumentation.


        EPU Software

        Thermo Scientific EPU Software is an embedded software for single particle acquisition. Its simplified user interface, advanced automation, and screening capabilities allow for straightforward planning and execution of experiments.

        Thermo Scientific Inspect 3D Software for 3D reconstruction

        Inspect 3D

        Thermo Scientific Inspect 3D Software provides a user-friendly solution for alignment of tomographic image series along with energy dispersive X-ray spectroscopy (EDS) maps, producing a 3D reconstruction of the data.


        Tomography 5 Software

        Thermo Scientific Tomography 5 Software delivers the latest advances in cryo-tomography, allowing you to acquire valuable 3D data even on your most challenging vitrified samples.


        EPU-D Software

        Thermo Scientific EPU-D Software is electron diffraction acquisition software. It enables quick switching between imaging and diffraction, guides optimal low-dose setup, and drives the continuous tilt for data collection.

        Glacios 2 Cryo-TEM accessories

        Selectris and Selectris X Imaging Filters

        Designed for stability and speed, Thermo Scientific Selectris and Selectris X Imaging Filters are post-column imaging filters that improve the contrast of TEM images, resulting in high-resolution structures up to atomic resolution. Paired with the latest generation Falcon 4i Direct Electron Detector, Selectris Filters enable you to obtain high-resolution structures quickly, revealing new biological insights. To further increase the productivity and ease of use of your cryo-EM research, these filters are fully integrated into Thermo Scientific EPU Software, Thermo Scientific Tomography Software, and instrument operation software.

        Selectris and Selectris X Imaging Filters

        Falcon 4i Direct Electron Detector

        The Thermo Scientific Falcon 4i Direct Electron Detector, with optical and throughput enhancement modes in combination with a more stable stage, accelerates automated data acquisition. Aberration-free image shift (AFIS) functionality uses image beam shifts, eliminating the need to perform mechanical stage movements. Fringe-free imaging (FFI) ensures the beam is only collecting data on usable parts of the sample, improving the quality of the results. When configured with the Selectris X Imaging Filter, the capability to use a very stable narrow slit width (<10 eV) provides an additional boost in contrast for single particle cryo-EM and cryo-ET, improving resolution and obtaining results faster with less data to maximize your resolution efforts. Paired with the Glacios Cryo TEM the Falcon 4i Direct Electron Detector makes it possible to efficiently solve structures routinely at 3.0 Å resolutions or less.

        Falcon 4i Direct Electron Detector for cryo-electron microscopy

        Ceta-D Camera

        The Thermo Scientific Ceta-D Camera is a scintillator-based camera optimized for low-dose diffraction data collection. It is ideal for working with dose-sensitive materials, such as proteins, pharmaceutical molecules, and organic materials. The camera’s high sensitivity allows for reliable detection of high-resolution, low-intensity diffraction peaks. Its high signal-to-noise ratio allows for accurate measurement of the integrated peak intensity. Both of these elements are prerequisites for obtaining high-resolution structural information.

        Ceta-D Camera for micro electron diffraction detector for cryo-EM

        Vitrobot Mark IV System

        The Thermo Scientific Vitrobot Mark IV System completely automates the vitrification process to provide fast, easy, reproducible sample preparation—the first step in obtaining high-quality images and repeatable experimental results.

        Vitrobot Mark IV System for sample vitrification

        On-axis BF/DF STEM Detector

        The Thermo Scientific Panther STEM Detector is a segmented dual sensor STEM imaging solution. It enables all state-of-the-art imaging techniques with its 16 segments. It is optimized for simultaneous multimodal STEM imaging in combination with spectral mapping applications. The high sensitivity and speed of the detector enable low-dose STEM imaging on beam-sensitive materials, dynamic STEM, and E/B field visualization via centerof mass (COM) analysis.

        Ceta-M Camera

        The Thermo Scientific Ceta-M Camera delivers sensitive and dynamic imaging with large field of view. This unique combination follows the natural workflow in TEM imaging: from fast navigation to find the area of interest to easy optimization of the image quality via optical adjustments to the final result, a 4k × 4k image unrivaled in quality and detail.

        Cryo-TEM instrument service and support

        Accelerate and Advance portfolios offer intensive on-site and remote support throughout the lifetime of your cryo-TEM workflow. During the warranty period, our Accelerate Service includes workflow validation and the support of our Customer Success Manager, who will help plan your installation, coordinate resources, and ensure you are set up for success. As you begin your research, our technologies make it easy for you to independently access resources while also facilitating a fast response from application experts when needed.

        Service at Thermo Fisher Scientific is a partnership, and we regularly deliver performance reviews to highlight areas where your system’s performance can be optimized. Additionally, you will receive access to both our Scientific Workflows App, which delivers step-by-step workflow guidance, as well as our Connected Care Portal, which provides on-demand reporting and information on system health and performance.

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        To ensure optimal system performance, we provide you access to a world-class network of field service experts, technical support, and certified spare parts.

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