Glacios 2 Cryo-TEM

The Thermo Scientific Glacios 2 Cryo-Transmission Electron Microscope (cryo-TEM) allows you to easily collect near atomic data from a broad range of biological targets. Compared to the previous generation, the Glacios 2 Cryo-TEM delivers higher throughput and makes cryo-EM more accessible. It features an integrated Thermo Scientific Falcon 4i Direct Electron Detector, Thermo Scientific EPU Software, and a new full enclosure, which combine to enhance image quality, automate data acquisition, and simplify your work. The Glacios 2 Cryo-TEM is ideally suited for single-particle analysis, cryo-electron tomography (cryo-ET), and micro-electron diffraction (MicroED).

See how the Falcon 4i Direct Electron Detector enhances throughput with 10 times shorter exposure times than previous generations.

Aberration-free image shift (AFIS) is an optical mode for faster data acquisition without comprising image quality. AFIS performs large beam shifts without off-axis coma and astigmatism.

Acquire more images per sample area with FFI, enhancing your daily data throughput. Hear how FFI has been helping the daily research in Dr. Elizabeth Villa’s lab.

Smart EPU Software enables faster and easier set up and processing, benefiting both expert and non-expert microscopists. Several critical set-up steps have been automated, such as alignment of the column and repair of system status, when possible. In addition, the software and various optional add-ons can help users get the most out of their microscope time and the best possible results.

• Optional Smart EPU Software with EPU Quality Monitor is an AI-enabled software solution capable of analyzing intermediate results, providing instant feedback and steering data collection on the fly.
• Optional Smart EPU Software with Embedded CryoSPARC Live is a turnkey solution for real-time structural feedback on data quality and comes with full service and application support.
• Optional EPU Multigrid Software allows data collection for up to 12 grids to be completed while away from the microscope, ensuring maximum productivity.
• Open application programming interface (API) enables the development of your own functionality for the Glacios 2 Cryo-TEM.

Selectris and Selectris X Imaging Filters

Designed for stability and speed, Thermo Scientific Selectris and Selectris X Imaging Filters are post-column imaging filters that improve the contrast of TEM images, resulting in high-resolution structures up to atomic resolution. Paired with the latest generation Falcon 4i Direct Electron Detector, Selectris Filters enable you to obtain high-resolution structures quickly, revealing new biological insights. To further increase the productivity and ease of use of your cryo-EM research, these filters are fully integrated into Thermo Scientific EPU Software, Thermo Scientific Tomography Software, and instrument operation software.

Falcon 4i Direct Electron Detector

The Thermo Scientific Falcon 4i Direct Electron Detector, with optical and throughput enhancement modes in combination with a more stable stage, accelerates automated data acquisition. Aberration-free image shift (AFIS) functionality uses image beam shifts, eliminating the need to perform mechanical stage movements. Fringe-free imaging (FFI) ensures the beam is only collecting data on usable parts of the sample, improving the quality of the results. When configured with the Selectris X Imaging Filter, the capability to use a very stable narrow slit width (<10 eV) provides an additional boost in contrast for single particle cryo-EM and cryo-ET, improving resolution and obtaining results faster with less data to maximize your resolution efforts. Paired with the Glacios Cryo TEM the Falcon 4i Direct Electron Detector makes it possible to efficiently solve structures routinely at 3.0 Å resolutions or less.

Ceta-D Camera

The Thermo Scientific Ceta-D Camera is a scintillator-based camera optimized for low-dose diffraction data collection. It is ideal for working with dose-sensitive materials, such as proteins, pharmaceutical molecules, and organic materials. The camera’s high sensitivity allows for reliable detection of high-resolution, low-intensity diffraction peaks. Its high signal-to-noise ratio allows for accurate measurement of the integrated peak intensity. Both of these elements are prerequisites for obtaining high-resolution structural information.

Vitrobot Mark IV System

The Thermo Scientific Vitrobot Mark IV System completely automates the vitrification process to provide fast, easy, reproducible sample preparation—the first step in obtaining high-quality images and repeatable experimental results.

On-axis BF/DF STEM Detector

The Thermo Scientific Panther STEM Detector is a segmented dual sensor STEM imaging solution. It enables all state-of-the-art imaging techniques with its 16 segments. It is optimized for simultaneous multimodal STEM imaging in combination with spectral mapping applications. The high sensitivity and speed of the detector enable low-dose STEM imaging on beam-sensitive materials, dynamic STEM, and E/B field visualization via centerof mass (COM) analysis.

Ceta-M Camera

The Thermo Scientific Ceta-M Camera delivers sensitive and dynamic imaging with large field of view. This unique combination follows the natural workflow in TEM imaging: from fast navigation to find the area of interest to easy optimization of the image quality via optical adjustments to the final result, a 4k × 4k image unrivaled in quality and detail.