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The new award-winning Thermo Scientific Krios G4 Cryo-Transmission Electron Microscope (Cryo-TEM) enables you to unravel life at the molecular level—easier, faster, and more reliably than ever before. The most compact TEM in its class, the Krios G4 Cryo-TEM consists of a highly stable 300 kV TEM platform and the industry-leading Autoloader (a cryogenic sample manipulation robot), making it ideally suited for automated applications such as single-particle analysis (SPA), cryo-electron tomography (cryo-ET) and micro-electron diffraction (microED).
Through a thorough redesign of the mechanical base frame and system enclosure, the microscope height has been reduced to below 3 m, which allows for instrument installation in labs with a ceiling height below 3.04 m (~10 ft), preventing costly room renovations. Built-in connectivity ensures a robust and risk-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing.
Solve structures at record-breaking resolutions
Pair the Krios G4 Cryo-TEM with the Thermo Scientific E-CFEG and the groundbreaking Thermo Scientific Selectris and Selectris X Imaging Filters to advance cryo-EM discovery with unprecedented resolution, speed, and ease of use.
The E-CFEG is a cold field emission gun with a narrow energy spread that results in higher contrast images and enables atomic resolution. This add-on makes it possible to efficiently solve structures at resolutions of 2.0 Å or less.
Selectris Imaging Filters provide unique stability, accessibility, and performance. Paired with the Thermo Scientific Falcon 4i Direct Electron Detector, these two add-ons produce better quality images up to 10 times faster than the previous generation.
An article published in Nature details how structural biologists used a Krios Cryo-TEM equipped with a Selectris Imaging Filter prototype to study protein structures at never-before-achieved resolutions, obtaining a 1.2 Å resolution structure of the iron-storing protein apoferritin and a 1.7 Å resolution of the GABA type A receptor-associated protein, with an even sharper resolution in key parts of the protein.
Already own a Krios Cryo-TEM? Explore upgrades and options ›
Solve more organic structures at record-breaking resolutions with the Thermo Scientific Krios G4 Cryo-TEM. Increase your lab’s productivity and performance with innovative upgrades.
Human Technopole established a new state-of-the-art cryo-electron microscopy (cryo-EM) facility , making cryo-EM access widely available to the scientific community. This advanced facility is equipped with several instruments, including the Thermo Scientific Krios G4 Cryo-TEM, Glacios Cryo-TEM, Talos L120C TEM, Aquilos 2 Cryo-FIB, and Spectra 300 S/TEM.
Easier to fit into new or existing labs
Redesigned internal base frame and system enclosure reduce height below 3 m (10 ft) while enhancing system performance and preventing costly room renovations.
Atomic resolution performance
The optional Selectris X Imaging Filter with Falcon 4i Direct Electron Detector and E-CFEG cold field emission source improve image contrast to reach higher resolutions in shorter time.
Maximized productivity through software automation
Advanced Performance Monitoring ensures the best optical starting point for automated runs and EPU software enables automated grid screening, quality monitoring, and data management.
Workflow connectivity
Designed for easy exchange of cassettes, facilitating a smooth transfer of specimens between Autoloader-equipped TEM instruments, and equipped with Athena Software to manage experimental parameters and data.
See the throughput impact of the Falcon 4i Direct Electron Detector with 10 times shorter exposure times than previous generations.
Aberration-free image shift (AFIS) is an optical mode for faster data acquisition without comprising image quality. AFIS performs large beam shifts without off-axis coma and astigmatism.
Acquire more images per sample area with fringe-free imaging (FFI), enhancing your daily data throughput. Hear how FFI has been helping the daily research in the lab of Dr. Elizabeth Villa.
Make the most of your day with enhanced automation for acquisition setup, system remote monitoring to optimize microscope utilization, on-the-fly imaging with quality monitoring, and data management and project administration.
Electron Source
- E-CFEG: Low-energy-spread cold field emission gun for higher contrast at truly atomic resolution
Detectors and Cameras
- Selectris X Imaging Filter: Zero-loss energy filtering for atomic resolution cryo-EM
- Falcon 4i direct electron detector
- Ceta-D microED detector
Software and Optical Modules
- Fringe-free imaging (FFI) optical module
- EPU Multigrid software
- EPU Quality Monitor software
Krios G4
- Pharma-dedicated solution for SPA and structure-based drug design
Accelerate your pathogen research with the 60°C heat decontamination solution
Empower your lab with the new 60°C heat decontamination for the Thermo Scientific Krios G4 cryo-transmission electron microscope (cryo-TEM). This accessory enables pathogen research and comprehensive instrument service in higher biosafety-level containment facilities (e.g. BSL3), so you can help make the world cleaner, safer, and healthier.
| Source | X-FEG (extreme high-brightness field emission gun) or low-energy-spread cold FEG (E-CFEG) |
| Accelerating voltage | 80–300 kV |
| Cryo-Autoloader | Automated and contamination-free loading of cassettes (up to 12 grids) |
| Temperature management software | Includes liquid nitrogen autofill and cool down scheduling |
| Lenses |
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| Stages |
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| Imaging | Rotation-free imaging with changing magnification |
| Advanced performance monitoring | Self-assessment of optical microscope status, combined with automated alignments, ensures ideal experimental conditions |
| Room Size Requirements (L × W × H) | 17’ × 22’ × 10’ |
| AFIS (aberration-free image shift) | Enhancing throughput with shorter relaxation times when moving coma-free between grid holes |
| FFI (fringe-free imaging) | Enhanced throughput with multiple image acquisitions per grid hole |
| Smart EPU Software |
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| Additional components |
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| Detectors (optional) |
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| Energy filter (optional) |
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| Other options |
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3D visualization of a Golgi apparatus from the green alga Chlamydomonas reinhardtii, created in Amira Software. The thin cryo-lamella of the sample was prepared with the Aquilos Cryo-FIB. Data courtesy of Dr. Benjamin Engel, Department of Molecular Structural Biology, Max Planck Institute for Biochemistry, Martinsried, Germany.
Histamine in the binding pocket of human membrane protein, GABA-A receptor (resolved at 1.7 Å). Data courtesy A. Sente and R. Aricescu, MRC-LMB Cambridge.
Atomic resolution structure of apoferritin, resolved by cryo-electron microscopy to 1.2 Å.
Cryo-EM structure of SARS-CoV-2 (COVID-19) spike glycoprotein in the prefusion conformation, with the three subunits of the trimer in red, green, and blue, and glycosylation in yellow. Image created with PDB data (6VSB).
Molecular architecture of the SARS-CoV-2 virus imaged by cryo-electron tomography. Courtesy of Sai Li, Tsinghua University.
Cryo-EM: What's Next. Register to view on-demand presentations covering the Selectris capabilities, a behind-the-scenes look at R&D filter development, and record-breaking structure resolution results for single particle analysis and cellular cryo-tomography using the Selectris Filter.
Solve more structures more easily at higher resolution with the next-generation Thermo Scientific Krios G4 Cryo-TEM.
Cryo-EM: Introducing a New Krios TEM Solution, presented by Dr. Marc Storms, Product Marketing Manager, Thermo Fisher Scientific.
Apoferritin is an iron-storing protein used to characterize cryo-EM performance. With the Selectris Imaging Filter, the position of individual hydrogen atoms, both in the protein and in surrounding water molecules, was resolved at 1.2 Å.
SARS-CoV-2 virion budding and assembly at the ERGIC membrane. A 3D volume rendering is shown with cellular and viral membranes in green and magenta respectively. Viral spike proteins are shown in yellow, and viral ribonucleoproteins in cyan. Courtesy of Chlanda lab, Schaller Research Groups, Department of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany. Visualization by Amira software.
Discover more with our new publications search tool and subscribe to our monthly publications newsletter.
Nakane, T., et al. Single-particle cryo-EM at atomic resolution. doi: 10.1038/s41586-020-2829-0
Thermo Scientific Krios Cryo-TEM publications associated with released maps. Source: EMDB database Jan 20, 2021.
3D visualization of a Golgi apparatus from the green alga Chlamydomonas reinhardtii, created in Amira Software. The thin cryo-lamella of the sample was prepared with the Aquilos Cryo-FIB. Data courtesy of Dr. Benjamin Engel, Department of Molecular Structural Biology, Max Planck Institute for Biochemistry, Martinsried, Germany.
Histamine in the binding pocket of human membrane protein, GABA-A receptor (resolved at 1.7 Å). Data courtesy A. Sente and R. Aricescu, MRC-LMB Cambridge.
Atomic resolution structure of apoferritin, resolved by cryo-electron microscopy to 1.2 Å.
Cryo-EM structure of SARS-CoV-2 (COVID-19) spike glycoprotein in the prefusion conformation, with the three subunits of the trimer in red, green, and blue, and glycosylation in yellow. Image created with PDB data (6VSB).
Molecular architecture of the SARS-CoV-2 virus imaged by cryo-electron tomography. Courtesy of Sai Li, Tsinghua University.
Cryo-EM: What's Next. Register to view on-demand presentations covering the Selectris capabilities, a behind-the-scenes look at R&D filter development, and record-breaking structure resolution results for single particle analysis and cellular cryo-tomography using the Selectris Filter.
Solve more structures more easily at higher resolution with the next-generation Thermo Scientific Krios G4 Cryo-TEM.
Cryo-EM: Introducing a New Krios TEM Solution, presented by Dr. Marc Storms, Product Marketing Manager, Thermo Fisher Scientific.
Apoferritin is an iron-storing protein used to characterize cryo-EM performance. With the Selectris Imaging Filter, the position of individual hydrogen atoms, both in the protein and in surrounding water molecules, was resolved at 1.2 Å.
SARS-CoV-2 virion budding and assembly at the ERGIC membrane. A 3D volume rendering is shown with cellular and viral membranes in green and magenta respectively. Viral spike proteins are shown in yellow, and viral ribonucleoproteins in cyan. Courtesy of Chlanda lab, Schaller Research Groups, Department of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany. Visualization by Amira software.
Discover more with our new publications search tool and subscribe to our monthly publications newsletter.
Nakane, T., et al. Single-particle cryo-EM at atomic resolution. doi: 10.1038/s41586-020-2829-0
Thermo Scientific Krios Cryo-TEM publications associated with released maps. Source: EMDB database Jan 20, 2021.
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Structural Biology Research
Cryo-electron microscopy enables the structural analysis of challenging biological targets such as large complexes, flexible species and membrane protein.
Drug Discovery
Learn how to take advantage of rational drug design for many major drug target classes, leading to best-in-class drugs.
Infectious Disease Research
Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.
Neurodegenerative Disease Research
Cryo-EM can determine the structural features of protein aggregates implicated in neurodegenerative diseases, allowing scientists to address how they form, interact with the cellular environment, and alter brain function.
Single Particle Analysis
Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.
Cryo-Tomography
Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.
MicroED
MicroED is an exciting new technique with applications in the structural determination of small molecules and protein. With this method, atomic details can be extracted from individual nanocrystals (<200 nm in size), even in a heterogeneous mixture.
Single Particle Analysis
Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.
Cryo-Tomography
Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.
MicroED
MicroED is an exciting new technique with applications in the structural determination of small molecules and protein. With this method, atomic details can be extracted from individual nanocrystals (<200 nm in size), even in a heterogeneous mixture.
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