Transmission Electron Microscopes

Cryo Electron Microscopy: Krios G4 Cryo-TEM

300 kV Cryo TEM with enhanced productivity and compact design.


The new Thermo Scientific Krios G4 Cryo Transmission Electron Microscope (Cryo-TEM) enables you to unravel life at the molecular level—easier, faster, and more reliably than ever before. The most compact TEM in its class, the Krios G4 Cryo-TEM consists of a highly stable 300 kV TEM platform and the industry-leading Autoloader (cryogenic sample manipulation robot), making it ideally suited for automated applications such as single particle analysis (SPA), cryo-electron tomography (cryoET) and micro electron diffraction (MicroED). Through a thorough redesign of the mechanical base frame and system enclosure, the microscope height has been reduced to below 3 meters, which allows for instrument installation in labs with a ceiling height below 3.04m (~10 ft), thereby avoiding costly room renovations. Designed-in connectivity ensures a robust and risk-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing.

Maximized productivity

Automated alignment routines ensure the microscope is tuned to its optimal starting point for data acquisition, which itself is accelerated up to 4x through the use of aberration-free image shifting along with the ability to collect more images per hole via a fringe free mode. Additionally, acquired data can be evaluated through on-the-fly monitoring of data quality and optimizing acquisition parameters as needed.

GABA receptor protein structure generated with the Krios Cryo-TEM and Amira Software. 3D reconstructions of the GABA receptor membrane protein in a nanodisk, bound to the drug Ro-15-4513. Displayed resolution is 2.75 Å. Data collected on a Krios Cryo-TEM with the Falcon 4 Detector. Images Courtesy of Simonas Masiulis, Radu Aricescu, MRC-LMB Cambridge and Evgenia Pechnikova, Abhay Kotecha, Thermo Fisher Scientific.

Key Features

Improved ergonomics

Easier sample exchange and data acquisition thanks to enhanced automation, systematic user guidance, and advanced performance monitoring.

Maximized productivity

A powerful combination of fringe free imaging (FFI), aberration-free image shift (AFIS), and the (optional) Falcon 4 Detector enhance SPA throughput significantly.

Workflow connectivity

Fits seamlessly into SPA cryoET, and MicroED workflows, with convenient and contamination-risk-free sample transfer throughout the workflow.

Fits more easily into new and existing labs

Avoid costly and challenging renovations with a completely redesigned mechanical base frame and system enclosure, resulting in a system height of <3 m.

Automation

Built-in self-diagnostic function (APM) evaluates microscope alignments for acquiring high-resolution data. Integrated Thermo Scientific EPU Software performs automatic daily tuning of essential alignments.

Best image quality for high-resolution 3D reconstruction

Improved information limit (0.12 nm) and linear distortion below 0.5% ensure the best possible boundary conditions for high-resolution imaging. This can be further improved with the Thermo Scientific Falcon 4 Detector and the Thermo Scientific Phase Plate Solution.


Specifications

Source X-FEG (extreme high-brightness field emission gun)
Accelerating voltage 80 – 300 kV
Cryo-Autoloader Automated and contamination-free loading of cassettes (up to 12 grids)
Temperature management software Includes liquid nitrogen autofill and cool down scheduling
Lenses
  • Automatic condenser, objective and SA apertures
  • Three-condenser lens system for automated, continuous parallel sample illumination
  • Symmetric constant power C-TWIN objective lens with wide-gap pole piece (11 mm)
Stages
  • Computerized 4-axis specimen stage with ±70-degree alpha tilt
  • Cryo-stage with single axis holder for optimized stability and drift performance
Imaging Rotation-free imaging upon changing magnification
Advanced performance monitoring Self-assessment of microscope status, combined with automated alignments, ensuring optimal experimental conditions
Room Size Requirements (L × W × H) 17’ × 22’ × 10’
AFIS (aberration-free image shift) Shorter relaxation time with shift between grid holes
FFI (fringe-free imaging) Multiple image acquisitions per grid hole
Thermo Scientific EPU 2 Software Automated SPA screening and data acquisition
Structural components
  • Two 30” monitors
  • Hand panels to be placed within 15 meters from the column, or extend up to 300 meters from the column (optional)
  • Environmental enclosure
Detectors (optional)
  • Falcon 3 Direct Electron Detector
  • Falcon 4 Direct Electron Detector
  • Ceta D Camera Ceta 16M Camera
  • HAADF STEM detectors
  • On-axis BF/DF detectors
Energy filter (optional) Gatan BioQuantum energy filter
Other options
  • Cs Image Corrector
  • Thermo Scientific Phase Plate Solution

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Golgi apparatus structure generated with the Krios Cryo-TEM and Amira Software.
3D visualization of a Golgi apparatus from the green alga Chlamydomonas reinhardtii, created in Amira Software. The thin cryo-lamella of the sample was prepared with the Aquilos Cryo-FIB. Data courtesy of Dr. Benjamin Engel, Department of Molecular Structural Biology, Max Planck Institute for Biochemistry, Martinsried, Germany.
Golgi apparatus structure generated with the Krios Cryo-TEM and Amira Software.
3D visualization of a Golgi apparatus from the green alga Chlamydomonas reinhardtii, created in Amira Software. The thin cryo-lamella of the sample was prepared with the Aquilos Cryo-FIB. Data courtesy of Dr. Benjamin Engel, Department of Molecular Structural Biology, Max Planck Institute for Biochemistry, Martinsried, Germany.

Applications

Life_Sciences_Application_274x180_144DPI

Structural Biology Research

Cryo-electron microscopy enables the structural analysis of challenging biological targets such as large complexes, flexible species and membrane protein.

DrugDiscovery_Appl_274x180

Drug Discovery

Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.

Infectious_Disease_Research_App_w274x180

Infectious Disease Research

Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.

Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

MicroED

MicroED is an exciting new technique with applications in the structural determination of small molecules and protein. With this method, atomic details can be extracted from individual nanocrystals (<200 nm in size), even in a heterogeneous mixture.

Learn more ›

Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

MicroED

MicroED is an exciting new technique with applications in the structural determination of small molecules and protein. With this method, atomic details can be extracted from individual nanocrystals (<200 nm in size), even in a heterogeneous mixture.

Learn more ›

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