Cryo Electron Microscope
The new Thermo Scientific Krios G4 Cryo Transmission Electron Microscope (Cryo-TEM) enables you to unravel life at the molecular level—easier, faster, and more reliably than ever before. The most compact TEM in its class, the Krios G4 Cryo-TEM consists of a highly stable 300 kV TEM platform and the industry-leading Autoloader (cryogenic sample manipulation robot), making it ideally suited for automated applications such as single particle analysis (SPA), cryo-electron tomography (cryoET) and micro electron diffraction (MicroED). Through a thorough redesign of the mechanical base frame and system enclosure, the microscope height has been reduced to below 3 meters, which allows for instrument installation in labs with a ceiling height below 3.04m (~10 ft), thereby avoiding costly room renovations. Designed-in connectivity ensures a robust and risk-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing.
Cryo-EM productivity
Automated alignment routines ensure the microscope is tuned to its optimal starting point for data acquisition, which itself is accelerated up to 4x through the use of aberration-free image shifting along with the ability to collect more images per hole via a fringe free mode. Additionally, acquired data can be evaluated through on-the-fly monitoring of data quality and optimizing acquisition parameters as needed.
The Thermo Scientific Krios Rx Cryo-TEM is the flagship of the iSPA Workflow, the first pharma-dedicated solution for single particle analysis (SPA) and structure-based drug design. This high-end microscope is optimized for the drug discovery process with a throughput of over 400 high-quality images per hour. The Krios Rx Cryo-TEM is configured for SPA only with a fixed voltage (300 kV), and comes standard equipped with a Falcon 4 detector. The simplified workflow with next-level EPU automations, including unattended multigrid data collection, makes it ideal for non-expert microscopists. The pharma-dedicated service package ensures the highest productivity and reliability with a 90% system uptime guarantee.
With a height less than 3 meters and an active vibration dampening system, the microscope fits in a standard room allowing easy installation and avoiding costly room renovations. The automated and programmable loading door also enables compatibility with robotic sample loading in the future.
Designed for stability and speed, the Thermo Scientific Selectris and Selectris X Imaging Filters are post-column imaging filters that improve the contrast of TEM images, resulting in high-resolution structures up to atomic resolution. Paired with the latest generation Thermo Scientific Falcon 4 Direct Electron Detector, Selectris Filters enable you to obtain high-resolution structures quickly, revealing new biological insights. To further increase the productivity and ease-of-use of your cryo-electron microscopy (cryo-EM) research, these filters are fully integrated into Thermo Scientific EPU Software, Thermo Scientific Tomography Software, and instrument operation software. The Selectris and Selectris X Imaging Filters are available on Thermo Scientific Krios and Glacios Cryo-TEMs.
Improved ergonomics
Easier sample exchange and data acquisition thanks to enhanced automation, systematic user guidance, and advanced performance monitoring.
Maximized productivity
A powerful combination of fringe free imaging (FFI), aberration-free image shift (AFIS), and the (optional) Falcon 4 Detector enhance SPA throughput significantly.
Workflow connectivity
Fits seamlessly into SPA cryoET, and MicroED workflows, with convenient and contamination-risk-free sample transfer throughout the workflow.
Fits more easily into new and existing labs
Avoid costly and challenging renovations with a completely redesigned mechanical base frame and system enclosure, resulting in a system height of <3 m.
Automation
Built-in self-diagnostic function (APM) evaluates microscope alignments for acquiring high-resolution data. Integrated Thermo Scientific EPU Software performs automatic daily tuning of essential alignments.
Best image quality for high-resolution 3D reconstruction
Improved information limit (0.12 nm) and linear distortion below 0.5% ensure the best possible boundary conditions for high-resolution imaging. This can be further improved with the Thermo Scientific Falcon 4 Detector and the Thermo Scientific Phase Plate Solution.
| Source | X-FEG (extreme high-brightness field emission gun) |
| Accelerating voltage | 80 – 300 kV |
| Cryo-Autoloader | Automated and contamination-free loading of cassettes (up to 12 grids) |
| Temperature management software | Includes liquid nitrogen autofill and cool down scheduling |
| Lenses |
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| Stages |
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| Imaging | Rotation-free imaging upon changing magnification |
| Advanced performance monitoring | Self-assessment of microscope status, combined with automated alignments, ensuring optimal experimental conditions |
| Room Size Requirements (L × W × H) | 17’ × 22’ × 10’ |
| AFIS (aberration-free image shift) | Shorter relaxation time with shift between grid holes |
| FFI (fringe-free imaging) | Multiple image acquisitions per grid hole |
| Thermo Scientific EPU 2 Software | Automated SPA screening and data acquisition |
| Structural components |
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| Detectors (optional) |
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| Energy filter (optional) | Gatan BioQuantum energy filter |
| Other options |
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Solve more structures more easily at higher resolution with the next-generation Thermo Scientific Krios G4 Cryo-TEM.
Cryo-EM: Introducing a New Krios TEM Solution
Presented by: Dr. Marc Storms Product Marketing Manager, Thermo Fisher Scientific
Solve more structures more easily at higher resolution with the next-generation Thermo Scientific Krios G4 Cryo-TEM.
Cryo-EM: Introducing a New Krios TEM Solution
Presented by: Dr. Marc Storms Product Marketing Manager, Thermo Fisher Scientific
Structural Biology Research
Cryo-electron microscopy enables the structural analysis of challenging biological targets such as large complexes, flexible species and membrane protein.
Drug Discovery
Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.
Infectious Disease Research
Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.
Single Particle Analysis
Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.
Cryo-Tomography
Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.
MicroED
MicroED is an exciting new technique with applications in the structural determination of small molecules and protein. With this method, atomic details can be extracted from individual nanocrystals (<200 nm in size), even in a heterogeneous mixture.
Single Particle Analysis
Single particle analysis (SPA) is a cryo-electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.
Cryo-Tomography
Cryo-electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.
MicroED
MicroED is an exciting new technique with applications in the structural determination of small molecules and protein. With this method, atomic details can be extracted from individual nanocrystals (<200 nm in size), even in a heterogeneous mixture.
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