kareem-faq-test-page-071922 | Thermo Fisher Scientific

Frequently asked questions (FAQs)

We do not recommend using NuPAGE Bis-Tris gels with NuPAGE MOPS or MES Running Buffer prepared without SDS for electrophoresis under native conditions. This buffer system may generate excessive heat, resulting in poor band resolution. Further, the protein of inter

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

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Yes, Super Bright-conjugated antibodies are stable in formaldehyde-based fixation buffers and permeabilization buffers, such as the IC Fixation and Permeabilization Buffer Set (Cat. No. 88-8824) and the Foxp3/Transcription Factor Staining Buffer Set (Cat. No. 00-5523).

Chlorobutanol is used as a preservative in the NuPAGE transfer buffer and is not necessary for efficient transfer of proteins. You may prepare the buffer without chlorobutanol but keep in mind that the buffer will not be stable for long periods. We recommend using it within 2 weeks.

Yes, we recommend adding the NuPAGE Antioxidant to the NuPAGE transfer buffer for enhanced blotting results with reduced proteins in order to maintain the reduced state of the proteins throughout the run.

August 11-for testing

Frequently asked questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

Can I use the NuPAGE transfer buffer with Tris-Glycine Gels?

Do I need to add the chlorobutanol when making the 20X NuPAGE Transfer Buffer?

Do you recommend adding the NuPAGE Antioxidant to the NuPAGE transfer buffer when I transfer proteins from NuPAGE Bis-Tris or NuPAGE Tris-Acetate gels?

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