HPLC Method Transfer Calculator

Use this online tool to transfer methods from HPLC to UHPLC conditions. The tool will calculate the new gradient table, adjust injection volumes, flow rates and run times if applicable. Please insert your current method conditions and find out how much time, sample and solvents you could save using UHPLC.

Use at your own risk. Prepopulated data are estimates only. Thermo Fisher Scientific takes no responsibility for the accuracy of data, calculations or results.

Current Method

Current Column

UHPLC Method

Planned Column
Peak Details (Critical Pair)
 

Peak Details (Critical Pair)
Current Method Conditions
Recommended Method Conditions

* Use this factor to increase the flow rate of the fast LC method. Note: if factor other than 1 is used, the resolution calculation is disabled.

0 bar

0 Hz

0

Current Gradient Table

Step Time (min) %A  %B  %C  %D 
1 100.0
2
3
4
5
6
7
8
9
10
End Time:0.000  
Recommended Reconditioning Time: n.a.

Planned Gradient Table

Time (min) %A  %B  %C  %D 
0.00 100.0
End Time:0.000  
Recommended Reconditioning Time: n.a.

 

Sample usage savings

Current UsagePlanned UsageSavings

Eluent usage and time savings

Current TotalPlanned TotalSavingsThroughput
Eluent Usage
Time

Total Time Savings

Total Eluent Savings

Total Sample Usage Savings