Thermo Fisher Scientific
In this webinar, Dr. Erwin Schoof will describe how a 384-well workflow, using standard off-the-shelf components, allows characterization of single cells to a protein depth of well over 1,000 proteins per cell. Dr. Schoof will introduce the latest generation HRAM Thermo Scientific™ Tribrid™ MS instrumentation with FAIMS technology for single cell-level sensitivity. When combined with new Thermo Scientific™ TMTPro reagents and optimized chromatography, researchers can analyze >100 single cells per day per instrument. An in-house developed computational pipeline, SCeptre, helps derive biological meaning from the data.
These advances in single cell MS sample preparation and analysis provide a robust way to collect cellular proteomes from mammalian cells, paving the way for large-scale single cell MS experiments at unprecedented depth.
Dr. Erwin Schoof, DTU Proteomics
Dr. Erwin Schoof heads the DTU Proteomics Facility while pursuing an academic career in cancer stem cell biology. He holds a joint appointment at DTU and the University of Copenhagen (in the lab of Prof. Bo Porse), focusing on normal and malignant hematopoiesis.
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