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        One Cell at a Time: Single Cell Proteomics Becomes a Reality

        Mass spectrometry (MS) workflows are poised to overcome limitations in our ability to identify and quantify proteins in single cells. In this webinar, Dr. Ryan Kelly will describe: nanoPOTS, a new sample processing platform to maximize sample recovery; miniaturization of LC separations; and latest-generation of MS instrumentation to provide the required sensitivity and throughput for effective analysis of such trace samples.

        Combined, these advances increase the sensitivity and throughput of single-cell MS by orders of magnitude making it possible to routinely quantify hundreds to >1000 proteins and proteoforms from an individual cell, transforming biomedical research and the understanding of biological systems on a new level.

        You will learn about:

        • How to optimize and minimize sample loss in single cell sample preparation for MS analysis
        • Best practices for single cell analysis with LC-MS
        • Using isobaric multiplexing strategies such as TMT for maximum throughput and sensitivity
        • Optimizing mass spec system for sensitivity using FAIMS technology

        About the presenter

        Ryan Kelly, Ph.D., Brigham Young University

        Ryan Kelly, Ph.D., Brigham Young University

        Dr. Ryan Kelly is an Associate Professor in the Department of Chemistry and Biochemistry at Brigham Young University and he holds a joint appointment at Pacific Northwest National Laboratory. His research combines microfluidics, advanced separations and mass spectrometry for ultrasensitive biochemical analyses including single cell omics and biochemical imaging.

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