Real time metabolite monitoring using the Ramina Process Analyzer and 500L HyPerforma Dynadrive Single Use Bioreactor

The Ramina Process Analyzer provides accurate in-line, real-time measurements of the critical process parameters (CPPs) of glucose, glutamine, and lactate as well as total and viable cell densities in the 500L HyPerforma DynaDrive (S.U.B.).

This application note describes the integration of the Ramina Process Analyzer with the 500L HyPerforma DynaDrive bioreactor to perform in-line measurements of these CPPs, and the development of accurate prediction models for several parameters and metabolites using this integrated system.

What you will learn in this article:

• The results of using Raman spectroscopy to monitor process parameters and the correlation of in-line spectral data to offline analytical data
• The cell culture and feeding strategy utilizing the Ramina Process Analyzer measurements and Chemometrics software
• The use of TouchRaman® immersion technology and HyPerforma DynaDrive S.U.B., for perfusion cell culture applications