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An LC-HRAM-MS method with Thermo Scientific TurboFlow online SPE was developed and validated for the quantitation of immunosuppressants. Online SPE efficiently extracts analytes while removing other components in a crude supernatant, significantly simplifying sample preparation. The use of full-scan data-dependent MS2 acquisition allows for high-resolution selected-ion monitoring quantitation of the analytes, confirming the analytes using MS2 fragmentation, as well as monitoring the signal intensities of major immunosuppressant metabolites.
For Research Use Only. Not for use in diagnostic procedures.
About the presenter
Ruben Luo, PhD, DABCC, Stanford University
Y. Ruben Luo, PhD, DABCC is an assistant professor of pathology at Stanford University and associate director of clinical chemistry laboratory at Stanford Health Care. He is dedicated to the innovation of clinical diagnostic technologies, and has pioneered the application of label-free immunoassays and top-down mass spectrometry in clinical chemistry. He completed his clinical chemistry fellowship at University of California San Francisco. Before the fellowship, he worked in the clinical diagnostic industry holding multiple managerial positions. He received his PhD in chemistry from Stanford University, and BS from Peking University.
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