AmpliTaq Gold® 360 DNA Polymerase Amplifies a Broad Range of Targets


Data produced with AmpliTaq Gold® 360 DNA Polymerase (A); the same amplicons amplified using the Sigma JumptStart™ DNA Polymerase (B). PCR reactions were performed using 1 ng of template DNA and 1.25 units of enzyme in each 50 µL reaction. Annealing was uniform across the selected targets. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 base pairs in length, with an average length of 553 bp. Each reaction was performed in duplicate. The high-GC target reactions included 2–10 µL of 360 GC Enhancer, depending on the target used.



Data produced with AmpliTaq Gold® 360 DNA Polymerase (A); the same amplicons amplified using the Qiagen HotStarTaq® DNA Polymerase (B). PCR reactions were performed using 1 ng of template DNA and 1.25 units of enzyme in each 50 µL reaction. Annealing was uniform across the selected targets. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Each reaction was performed in duplicate. The high-GC target reactions included 2–10 µL of 360 GC Enhancer, depending on the target used.