Specificity Comparison of Platinum® Taq DNA Polymerase to Another Supplier’s Polymerase.
The data show a summary of two replicates for each of 40 amplicons, indicating the average specificity for each amplicon and the standard deviation. Platinum® Taq PCR reactions were performed using 1 ng of template DNA per reaction and the manufacturer’s recommended cycling conditions. Annealing and extension times and temperatures were specific to each primer set. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Data shown here compare Platinum® Taq DNA Polymerase (red) with Sigma JumpStart™ Taq DNA Polymerase (green).