Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Life Technologies offers you gold-standard Applied Biosystems® real-time PCR instrumentation at a price that’s more affordable than ever, with systems starting at just $9,995. You can perform experiments using 48 wells, 96 wells, 384 wells, or higher, knowing your system is supported by world-class service and support.
Designed for compatibility with TaqMan® Assays as well as the flexibility of extensive real-time PCR chemistry choices, we make it easy for you to get started and get the results you need. We have a solution that meets every lab's needs.
Easy to use for all levels of experience | High flexibility & intuitive design |
Maximum throughput,
flexibility and scalability | |
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Easy to use for all levels
of experience | ![]()
Powerful features &
intuitive design | ![]()
Maximum throughput,
flexibility & scalability | |
Reactions per Run | Up to 48/96 | Up to 384 | Up to 12000+ |
Block Format(s) | 48/96 | 96, 96 Fast, 384, TaqMan Array (interchangeable) | 96, 96 Fast, 384, TaqMan Array, OpenArray
(interchangeable) |
Color Combinations (Multiplexing) | 3 and 4 | 21 | 21 |
Run Time | As fast as 40 min. | As fast as 30 min. | As fast as 30 min. |
Programmable Volumes | 1-40ul | 1-200uL* | 1-200uL* |
Validated Volumes | 10-40ul | 1-100uL* | 33nl-100uL* |
Automation Compatible | No | Twister Robot Available | Twister Robot Available |
Remote Monitoring | Yes | Yes | Yes |
On-Board Memory | Yes — 1 Run | Yes — 100+ Runs | Yes — 100+ Runs |
Email Notification | No | Yes | Yes |
Calendar Scheduling | No | Yes | Yes |
Assist with 21 CFR p 11 compliance | Not available | Security, Auditing, eSignature packages available | |
Sensitivity | Down to 1 copy | ||
Dynamic Range | Up to 9 logs | ||
Chemistry | All real-time PCR based chemistries. Flexible to chemistries with or without passive reference dye. | ||
Applications Available | Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, microRNA | Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, microRNA | Gene Expression, Genotyping, Copy Number Variation, HRM, Protein Thermal Shift, Protein Detection, microRNA, digital PCR |
Validated Applications | N/A | N/A | N/A |
IVD Platform | No | Available in certain countries | No |
Instruments
| |||
Temperature uniformity | ± 0.5°C within 30 seconds of clock start | ±0.5°C, within 30 sec of reaching temperature set point | ±0.5°C |
Optics | |||
Excitation source | Blue LED | OptiFlex™ System with Halogen lamp | OptiFlex™ System with White LED light |
Filters/Colors | 4 filters factory calibrated for FAM™ / SYBR® Green, VIC® / JOE™, NED™ / TAMRA™ and ROX™ / dyes | 6 excitation filters (450—670 nm)
6 emission filters (500—720 nm) | 6 excitation filters (450—670 nm)
6 emission filters (500—720 nm) |
Detector | Photodiodes | CCD | CCD |
Instrument specs
| |||
Size | W 23.6 cm
D 51.2 cm H 42.7 cm | W 53.3 cm
D 63.5 cm H 64.5 cm | W 50.5 cm
D 67.2 cm H 73.8 cm |
Gives the foundation for learning real-time PCR technology.
Describes real-time PCR reagents and which applications they are best used for.
Focuses on how to choose the best assays for real-time quantitative PCR experiments, particularly for gene expression and miRNA's.