Master Mix

Applied Biosystems VetMAX Fast Multiplex Master Mix is a highly sensitive master mix compatible with fast cycling conditions and optimized for inhibitory animal samples.

 Benefits

• Compatible with Fast cycling (<50-minute thermal cycle run time)
• Compatible with a variety of assays and complexities (RNA, DNA, singleplex, multiplex)
• Ability to tolerate inhibitors from a variety of sample types such as fecal, environmental, blood, milk, oral fluid, etc.
• Single-tube format for ease of use

Formulation

VetMAX Fast Multiplex Master Mix contains:

• A concentrated M-MLV RT capable of producing high cDNA yields
• Concentrated ultrapure hot-start DNA polymerase providing superior specificity and sensitivity
• A fast-cycling-optimized 2X RT-PCR buffer for inhibitor tolerant, multiplex-compatible reverse transcription and PCR
• Invitrogen ROX dye as an internal reference for normalization and precise data analysis

Performance

VetMAX Fast Multiplex Master Mix (with ROX) is a fast-cycling, multiplex-assay-compatible master mix that has enhanced multiplexing capability, sensitivity, and inhibitor tolerance. It helps decrease time-to-results while providing higher order multiplexing capabilities and enhanced inhibitor tolerance so you can analyze up to four targets from a single reaction, regardless of sample type. The single-tube format saves hands-on time so you need only add your assay and sample and go.

The Applied Biosystems AgPath-ID One-Step RT-PCR Kit is an economical, high-quality, ready-to-use master mix for amplification of RNA targets. The kit is designed for sensitive, robust amplification of RNA targets in the presence of PCR inhibitors typically found in animal samples.

The Applied Biosystems Path-ID Multiplex One-Step RT-PCR Kit is a highly sensitive and convenient master mix optimized for veterinary labs targeting RNA pathogens. The Path-ID Multiplex One-Step RT-PCR Kit is designed for the sensitive, robust amplification and multiplex quantitation of animal pathogen RNA in a simple format.

Benefits

• Mutliplex amplification of up to 4 different targets simultaneously helps save time and money
• Optimized to amplify low copy (20 copies) number targets to enable results even with challenging samples
• Capable of amplification of over 7 logs of input to provide robust performance when you need it

Formulations

• Multiplex Enzyme Mix containing an M-MLV reverse transcriptase capable of producing high cDNA yields, and an ultra-pure, hot-start DNA polymerase which provides superior specificity and sensitivity
• Multiplex RT-PCR Buffer with optimized reagents for efficient, robust results from both the reverse transcription reaction and the PCR. Includes ROX dye as an internal reference for normalization and precise data analysis

Multiplex with confidence

Applied Biosystems Path-ID qPCR Master Mix is a highly sensitive master mix used to detect animal pathogen DNA, optimized to perform in the presence of challenging qPCR inhibitors. Path-ID qPCR Master Mix is designed for the sensitive, robust amplification of animal pathogen DNA in a convenient format.

Benefits

• Capable of amplifying over 7 logs of input and down to 25 copies of target for dependable, robust performance
• Inhibitor tolerance to help deliver accurate results even with challenging samples
• Stable performance at a wide temperature range allows for convenient reaction setup and reagent storage

Formulations

• Ultra-pure, hot-start DNA polymerase enables room temperature reaction setup and minimizes nonspecific PCR products
• Optimized buffer and dNTPs for enhanced sensitivity and functionality in the presence of PCR inhibitors
• ROX dye as an internal reference for normalization and precise data analysis

Convenience and performance

Applied Biosystems VetMAX-Plus master mix solutions provide the highly sensitive and robust performance you need with the added confidence and convenience of an included Xeno internal positive control (IPC). The use of an IPC in pathogen detection workflows allows you to distinguish true target negatives from PCR inhibition.

Formulations

VetMAX-Plus One-Step RT-PCR Kit

• 25X RT-PCR Enzyme Mix that contains ArrayScript Reverse Transcriptase (a mutant M-MLV RT capable of producing high cDNA yields), and an ultra-pure, hot-start DNA polymerase which offers superior specificity and sensitivity
• 2X RT-PCR Buffer for efficient, robust reverse transcription and PCR. Includes ROX dye as an internal reference for normalization and precise data analysis
• Xeno RNA Control

VetMAX-Plus Multiplex One-Step RT-PCR Kit

• 10X Multiplex Enzyme Mix that contains an M-MLV reverse transcriptase capable of producing high cDNA yields, and an ultra-pure, hot-start DNA polymerase which provides superior specificity and sensitivity
• 2X Multiplex RT-PCR Buffer for efficient, robust reverse transcription and PCR. Includes ROX dye as an internal reference for normalization and precise data analysis
• Xeno RNA Control

VetMAX-Plus qPCR Master Mix

• 2X qPCR Master Mix that contains an ultra-pure, hot-start DNA polymerase which enables room temperature reaction setup and minimizes nonspecific PCR products
• Optimized buffer and dNTPs for enhanced sensitivity and functionality in the presence of PCR inhibitors, and ROX dye as an internal reference for normalization and precise data analysis
• Xeno DNA Control

For veterinary use only.