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Master Mix
Master mix solutions that are built for the specific needs of veterinary diagnostic labs
The value of your PCR testing results is only as good as the reagents you rely on. That’s why we optimized our enzymes to help you identify the animal pathogen targets most important to you.
We have rigorously developed reagents that offer robust, consistent performance—even in the presence of PCR inhibitors found in the most challenging animal samples.
Whether your lab’s needs are simple or complex, you are new to PCR testing or have been designing assays for years, our easy-to-use master mixes can help you feel confident in your results.
One-step RT-PCR master mix
Applied Biosystems AgPath-ID One-Step RT-PCR kit is an economical, high-quality, ready-to-use master mix for amplification of RNA targets. The kit is designed for sensitive, robust amplification of RNA targets in the presence of PCR inhibitors typically found in animal samples.
Benefits
- Consistent, reliable amplification, for results you can trust
- Simple, single-tube, one-step reaction minimizes handling and helps reduce the risk of cross contamination
- Detection Enhancer is provided as an optional reagent for amplification of difficult templates
Sensitive, reliable performance
Formulations
- 25X RT-PCR Enzyme Mix that contains Applied Biosystems ArrayScript Reverse Transcriptase (a mutant M-MLV RT that produces high cDNA yields), and Ultra-pure, Hot- Start DNA polymerase, which offers superior specificity and sensitivity
- Optimized 2X RT-PCR Buffer for efficient, robust reverse transcription and PCR. Includes ROX dye as an internal reference for normalization and precise data analysis
- Detection Enhancer as an optional reagent for amplification of templates with high GC content or persistent secondary structure
Multiplex one-step RT-PCR master mix
Applied Biosystems Path-ID Multiplex One-Step RT-PCR Kit is a highly sensitive and convenient master mix optimized for veterinary labs targeting RNA pathogens. The Path-ID Multiplex One-Step RT-PCR Kit is designed for the sensitive, robust amplification and multiplex quantitation of animal pathogen RNA in a simple format.
Benefits
- Mutliplex amplification of up to 4 different targets simultaneously helps save time and money
- Optimized to amplify low copy (20 copies) number targets to enable results even with challenging samples
- Capable of amplification of over 7 logs of input to provide robust performance when you need it
Formulations
- Multiplex Enzyme Mix containing an M-MLV reverse transcriptase capable of producing high cDNA yields, and an ultra-pure, hot-start DNA polymerase which provides superior specificity and sensitivity
- Multiplex RT-PCR Buffer with optimized reagents for efficient, robust results from both the reverse transcription reaction and the PCR. Includes ROX dye as an internal reference for normalization and precise data analysis
Multiplex with confidence
qPCR master mix
Applied Biosystems Path-ID qPCR Master Mix is a highly sensitive master mix used to detect animal pathogen DNA, optimized to perform in the presence of challenging qPCR inhibitors. Path-ID qPCR Master Mix is designed for the sensitive, robust amplification of animal pathogen DNA in a convenient format.
Benefits
- Capable of amplifying over 7 logs of input and down to 25 copies of target for dependable, robust performance
- Inhibitor tolerance to help deliver accurate results even with challenging samples
- Stable performance at a wide temperature range allows for convenient reaction setup and reagent storage
Formulations
- Ultra-pure, hot-start DNA polymerase enables room temperature reaction setup and minimizes nonspecific PCR products
- Optimized buffer and dNTPs for enhanced sensitivity and functionality in the presence of PCR inhibitors
- ROX dye as an internal reference for normalization and precise data analysis
Convenience and performance
Master mixes with internal positive control (IPC)
Applied Biosystems VetMAX-Plus master mix solutions provide the highly sensitive and robust performance you need with the added confidence and convenience of an included Xeno internal positive control (IPC). The use of an IPC in pathogen detection workflows allows you to distinguish true target negatives from PCR inhibition.
Benefits
- Xeno IPC monitors the reaction for inhibition and effectiveness of nucleic acid purification, enabling greater confidence in results
- Formulations are optimized for use in detecting challenging animal RNA or DNA pathogens
- A suite of master mix options (RT-PCR, multiplex, qPCR) are available to fit your unique application
Qualified results
Formulations
Applied Biosystems VetMAX-Plus One-Step RT-PCR Kit
- 25X RT-PCR Enzyme Mix that contains ArrayScript Reverse Transcriptase (a mutant M-MLV RT capable of producing high cDNA yields), and an ultra-pure, hot-start DNA polymerase which offers superior specificity and sensitivity
- 2X RT-PCR Buffer for efficient, robust reverse transcription and PCR. Includes ROX dye as an internal reference for normalization and precise data analysis
- Xeno RNA Control
Applied Biosystems VetMAX-Plus Multiplex One-Step RT-PCR Kit
- 10X Multiplex Enzyme Mix that contains an M-MLV reverse transcriptase capable of producing high cDNA yields, and an ultra-pure, hot-start DNA polymerase which provides superior specificity and sensitivity
- 2X Multiplex RT-PCR Buffer for efficient, robust reverse transcription and PCR. Includes ROX dye as an internal reference for normalization and precise data analysis
- Xeno RNA Control
Applied Biosystems VetMAX-Plus qPCR Master Mix
- 2X qPCR Master Mix that contains an ultra-pure, hot-start DNA polymerase which enables room temperature reaction setup and minimizes nonspecific PCR products
- Optimized buffer and dNTPs for enhanced sensitivity and functionality in the presence of PCR inhibitors, and ROX dye as an internal reference for normalization and precise data analysis
- Xeno DNA Control
Master mix product flyer
Our vision is to provide comprehensive solutions to help diagnose farm animal health issues more quickly and accurately than ever before—and so enhance animal health and productivity. To learn more about our master mix solutions, please download our product flyer or contact a product specialist for further assistance.
For veterinary use only.