BovineMastitis
VetMAX MastiType multiplex qPCR kits enable immediate and informed action

Because bovine mastitis can cause significant losses, farmers and herd managers need quick diagnostic results so they can take fast and appropriate action. Traditionally, culture has been used for the diagnosis of mastitis infections, but results can take a long time and pathogens can be difficult to grow, especially for Mycoplasma species.

Our multiplex qPCR and sample extraction solutions, the Applied Biosystems VetMAX MastiType multiplex qPCR kits, together with the Applied Biosystems MagMAX CORE Nucleic Acid Purification Kit, are designed to provide same-day results even for Mycoplasma spp., enabling farmers and veterinarians to take immediate and well-informed action.

Advantages:

  • Faster results—typical time from sample to result is 2.5–3 hours, compared to up to 10 days for a culture
  • No bacterial culture needed—detection independent of viable bacteria as PCR detects DNA
  • Detection of growth-inhibited pathogens—e.g., when treatment was already started
  • Detection of mastitis pathogens before mastitis is clinically apparent
  • Convenience—sample extraction does not require aspiration and centrifugation
  • Highly accurate—high sensitivity and specificity for all individual pathogens
  • Superior customer support—support from our experienced animal health service and support team
  • Leverage our experience—large portfolio of veterinary diagnostics
Featured products
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MagMAX CORE Nucleic Acid Purification Kit and Mastitis & Panbacteria Module

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VetMAX MastiType Micro4, Myco8 or Multi multiplex qPCR kits

VeriVet software

Applied Biosystems VeriVet software

Nucleic acid extraction

MagMAX CORE Nucleic Acid Purification Kit and Mastitis & Panbacteria Module—for fast and convenient nucleic acid extraction from milk samples, yielding high-quality nucleic acids.

The MagMAX CORE Mastitis & Panbacteria module is offered in combination with the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of bacteria important in the detection of mastitis in cattle and from challenging matrices like mastitic milk.

The Applied Biosystems MagMAX CORE sample extraction system is a universal magnetic bead-based separation system designed to meet current and future veterinary testing needs. It is compatible with both automated (using the KingFisher Flex Purification System) and manual purification methods. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing.

With the MagMAX CORE Mastitis & Panbacteria module, we have included a specially optimized lysis solution, along with altering the extraction procedure to improve the recovery of nucleic acid from all bacteria, including gram positive bacteria.

Features include:

  • Convenient—a complete solution using the same CORE chemistry
  • Easy—no centrifugation, no aspiration steps
  • Fast—less than one hour for 96 samples
  • Flexible—appropriate for fresh, frozen, or preserved milk samples, including samples with high somatic cell count

The MagMAX CORE kit and the MagMAX CORE Mastitis & Panbacteria Module help make your lab more efficient through greater convenience, consistent performance, and time and cost savings. The kit uses a magnetic bead-based separation system.


Amplification

The VetMAX MastiType Micro4 Kit offers real-time PCR detection of mastitis-causing pathogens in a single-well real-time PCR reaction. The VetMAX MastiType Micro4 assay enables rapid, accurate detection of the major contagious mastitis-causing pathogens in bovine milk using real-time PCR amplification of DNA unique to each pathogen.

  • Mycoplasma bovis
  • Staphylococcus aureus
  • Streptococcus agalactiae
  • Streptococcus uberis

Regulatory requirements vary by country; products may not be available in your geographic area.

VetMAX MastiType Myco8 Kit offers real‑time PCR detection of eight mastitis-causing pathogens in two separate PCR reactions. The VetMAX MastiType Myco8 assay enables rapid, accurate detection of mastitis-causing pathogens, especially the highly contagious and difficult-to-culture mycoplasma strains in bovine milk, using real-time PCR amplification of DNA unique to each pathogen.

  • Mycoplasma spp.
  • Mycoplasma alkalescens
  • Mycoplasma bovis
  • Mycoplasma bovigenitalium
  • Mycoplasma canadense
  • Mycoplasma californicum
  • Staphylococcus aureus
  • Streptococcus agalactiae

Regulatory requirements vary by country; products may not be available in your geographic area.

The VetMAX MastiType Multi Kit offers real-time PCR detection of all major mastitis-causing pathogens and a penicillin-resistance gene in four separate PCR reactions. The VetMAX MastiType Multi assay helps to enable rapid, accurate detection of 15 major mastitis-causing pathogens in bovine milk using real-time PCR amplification of DNA unique to each pathogen.

  • Staphylococcus aureus
  • Staphylococcus spp. (including all major coagulase-negative staphylococci)
  • Streptococcus agalactiae
  • Streptococcus dysgalactiae
  • Streptococcus uberis
  • Escherichia coli
  • Enterococcus spp. (incl. E. faecalis and E. faecium)
  • Klebsiella oxytoca and/or K. pneumoniae
  • Serratia marcescens
  • Corynebacterium bovis
  • Trueperella pyogenes and/or Peptoniphilus indolicus
  • Staphylococcal ß-lactamase gene (penicillin-resistance gene)
  • Mycoplasma bovis
  • Mycoplasma spp.
  • Yeast
  • Prototheca spp.

The VetMAX MastiType Micro4 Kit offers real-time PCR detection of mastitis-causing pathogens in a single-well real-time PCR reaction. The VetMAX MastiType Micro4 assay enables rapid, accurate detection of the major contagious mastitis-causing pathogens in bovine milk using real-time PCR amplification of DNA unique to each pathogen.

  • Mycoplasma bovis
  • Staphylococcus aureus
  • Streptococcus agalactiae
  • Streptococcus uberis

Regulatory requirements vary by country; products may not be available in your geographic area.

VetMAX MastiType Myco8 Kit offers real‑time PCR detection of eight mastitis-causing pathogens in two separate PCR reactions. The VetMAX MastiType Myco8 assay enables rapid, accurate detection of mastitis-causing pathogens, especially the highly contagious and difficult-to-culture mycoplasma strains in bovine milk, using real-time PCR amplification of DNA unique to each pathogen.

  • Mycoplasma spp.
  • Mycoplasma alkalescens
  • Mycoplasma bovis
  • Mycoplasma bovigenitalium
  • Mycoplasma canadense
  • Mycoplasma californicum
  • Staphylococcus aureus
  • Streptococcus agalactiae

Regulatory requirements vary by country; products may not be available in your geographic area.

The VetMAX MastiType Multi Kit offers real-time PCR detection of all major mastitis-causing pathogens and a penicillin-resistance gene in four separate PCR reactions. The VetMAX MastiType Multi assay helps to enable rapid, accurate detection of 15 major mastitis-causing pathogens in bovine milk using real-time PCR amplification of DNA unique to each pathogen.

  • Staphylococcus aureus
  • Staphylococcus spp. (including all major coagulase-negative staphylococci)
  • Streptococcus agalactiae
  • Streptococcus dysgalactiae
  • Streptococcus uberis
  • Escherichia coli
  • Enterococcus spp. (incl. E. faecalis and E. faecium)
  • Klebsiella oxytoca and/or K. pneumoniae
  • Serratia marcescens
  • Corynebacterium bovis
  • Trueperella pyogenes and/or Peptoniphilus indolicus
  • Staphylococcal ß-lactamase gene (penicillin-resistance gene)
  • Mycoplasma bovis
  • Mycoplasma spp.
  • Yeast
  • Prototheca spp.

Result interpretation

VeriVetSoftware-icon

Applied Biosystems VeriVet Software
Simplify, accelerate, and standardize data analysis with our result interpretation software. The cloud-based Animal Health VeriVet App allows you to create a plate layout, generate a template file for import to the real-time PCR instrument, and remotely monitor the instrument run.

  • Innovative cloud-based data analysis and storage
  • Use with QuantStudio 5 and 7500 Fast PCR instruments
  • Easy readout of positive and negative results
  • Fully automated analysis
  • Fast, clear view of results/calls
  • No need to interpret curves or Cts
  • No manual adjustments and calculations needed
  • Inhibited samples identified


Mastitis diagnosis workflow

Bovine mastitis diagnosis

Bovine mastitis is the most common and costly disease among dairy cows. It is an inflammation of the mammary gland that is predominantly caused by a bacterial infection. When mastitis is identified in a cow’s quarter(s), it is important to identify the pathogen causing the infection because different categories of pathogens require different management strategies. Once the infection is identified, a dairy farmer can work with his or her veterinarian to target an effective treatment plan. Prudent use of antibiotics reduces the likelihood of resistant pathogens developing and can reduce the duration of treatment a cow may need, which in turn decreases operation costs.

Bovine mastitis diagnosis

Mastitis cost

Mastitis is estimated to cost the global dairy industry US $19.7 to US $32 billion annually.

With clinical mastitis, milk is typically visibly watery, thick or ropy, flakey, flocky, containing pus and blood or clots and often the quarter is red, swollen, and painful. A case of clinical mastitis may lead to an economic loss that ranges somewhere between US $128 (€105) and US $444 ( €360) (Rollin et al. 2015; Cha et al. 2011; Huijps et al. 2008)

Subclinical mastitis is not visibly notable when looking at the milk, and the udder appears normal. However, the somatic cell count of the cow’s milk is slightly elevated, and microorganisms can usually be detected in a diagnostic sample. The overall product loss of subclinical mastitis is estimated at US $110 per cow annually. The greatest economic losses are attributed to high bulk tank somatic cell count resulting in loss of quality premiums and overall lower milk production.

The cost of M. bovis in cattle due to mastitis is estimated at US $108 million annually with infection rates of up to 70% of a herd, for pneumonia and related respiratory illnesses at US $32 million annually. Total M. bovis estimated disease cost is at least US $140 million annually in the U.S. Even higher losses—estimated at €144 to €192 million—have been reported in Europe.



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