An FLI-approved, real-time PCR kit for the early detection of BVDV in persistently infected (PI) calves
Thermo Fisher Scientific animal health products have been at the forefront of recent advances in production animal diagnostics, and supply some of the leading modern diagnostic tools to veterinary laboratories around the world.
These innovative diagnostic solutions include our FLI-approved (FLI-B 649) LSI VetMAX™ BVDV 4ALL Detection Kit: a rapid, easy-to-use PCR-based testing solution that detects type 1-3 bovine viral diarrhea virus (BVDV) and type 1-6 border disease virus (BD) in cattle with a high degree of specificity and sensitivity.
Bovine viral diarrhea virus (BVDV) is endemic in many cattle-producing countries, and contributes to considerable economic loss within the beef and dairy industries. Cattle of all ages are susceptible to BVDV, with most cases of overt clinical disease seen in cattle between 6 months and 2 years old.
The virus crosses the placenta in infected pregnant cows, causing reproductive losses due to abortion, stillborn calves, or calves that die early in life. When cows become infected between days 30 and 150 of gestation, surviving calves become persistently infected (PI) with BVDV. PI cattle are the main source of BVDV transmission, shedding large amounts of the virus in their secretions and excretions. As a result, most eradication and control programs focus on elimination of PI animals and preventing the transmission of BVDV to healthy cattle within a herd.
The LSI VetMAX™ BVDV 4ALL Detection Kit is FLI-approved, registered with the ANSES BVDV-free certification program (ear notches/France), and has been successfully implemented within several European programs where the kit has consistently detected BVDV with a high level of specificity, sensitivity, repeatability, and reproducibility.
Veterinary diagnosticians have relied on our molecular reagents and instrumentation to detect and control disease, and with our LSI VetMAX™ BVDV 4ALL product they have a proven diagnostic test for bovine viral diarrhea virus that helps with the rapid detection of persistently infected animals and the preservation of herd health.
Highly specific and sensitive detection of BVDV types 1–3, 100% detection of young PI calves, and positive extraction/amplification controls that help ensure reliable results for all sample matrices.
A simple, validated, and easily automated workflow helps minimize sample handling and cross-contamination to deliver a fast, cost-effective BVDV detection method for all matrices (blood, serum, ear notches, milk).
The kit is validated with purification for use with single samples or with pooled samples of up to 50 (blood/serum) and up to 100 (ear notches), and offers an automated solution for high-throughput testing environments.
|Details||LSI VetMAX™ BVDV 4ALL kit|
|Sample preparation||Fast Lysis, MagMAX™ Pathogen RNA/DNA Kit and MagVET™|
|Species||Bovine, sheep, goat, and wild ruminants|
|Sample matrices||Blood, serum, ear notch, and milk|
|Pooling||With sample extraction, up to 50 blood/serum samples and up to 100 ear notches|
|Virus type detected||BVDV types 1–3 and border disease virus type 1–6|
|Limit of detection||40 copies|
|Internal positive control||Endogenous or exogenous|
|Licensing||FLI-approved (FLI-B 649)|
Our vision is to provide comprehensive solutions to help diagnose farm animal health issues more quickly and accurately than ever before—and so enhance animal health and productivity. To learn more about our LSI VetMAX™ BVDV 4ALL Detection Kit, please download our product flyer.